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Chromogenic counting medium for mycete and microzyme

A culture medium and yeast technology, applied in the field of culture medium, can solve the problems of long culture time, unfavorable count of bacteria, large colony, etc., and achieve the effect of short culture time, favorable count of bacteria and small colony

Inactive Publication Date: 2014-04-16
STONE LAKE PHARMA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Mold and yeast widely exist in the air, water and soil. Most of them are harmless to the human body, but some molds are harmful to the human body. Mold can also cause mildew in food, stimulate the human digestive tract, stomach, etc. Can damage the liver and cause food poisoning
Mold and yeast use potato white sugar agar (PDA), Sabouraud's agar medium (Sabouraud's agar), etc., these medium culture time is too long, the colonies are too large, which is not conducive to counting the number of bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] Mold yeast chromogenic medium, consisting of 20g of peptone, 5g of glucose, 10g of sodium chloride, 0.01g of colistin sulfate, 0.3g of chloramphenicol, 0.01g of tetrazolium violet and 20g of agar dissolved in 1000ml of deionized water, After high temperature and high pressure sterilization, the chromogenic culture medium was prepared.

[0010] Further, the culture medium for colony chromogenic counting is a plate culture medium or a test paper type or a detection sheet type.

[0011] Principle of the present invention is:

[0012] 1. Each 1000ml medium contains peptone 10~20g, glucose 1~20g, sodium chloride 5~10g, colistin sulfate 0.001~0.1g, chloramphenicol 0.01~0.5g, TTV (tetrazolium violet) 0.005 ~0.5, agar 15~20g.

[0013] 2. Specific implementation: Take 20g of peptone, 5g of glucose, 10g of sodium chloride, 0.01g of colistin sulfate, 0.3g of chloramphenicol, 0.01g of tetrazolium violet (TTV), and 20g of agar, and dissolve them in 1000ml of deionized water. Afte...

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Abstract

The invention discloses a chromogenic counting medium for mycete and microzyme, and the chromogenic counting medium is prepared as follows: dissolving 20g of peptone, 5g of glucose, 10g of sodium chloride, 0.01g of colistin sulphate, 0.3g of chloramphenicol, 0.01g of tetrazolium violet and 20g of agar in 1000ml deionized water for high temperature and high pressure sterilization to prepare a chromogenic plate medium. The chromogenic counting medium is short in culture time, small in bacterial colony, and beneficial for bacterial colony counting.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for color development and counting of mold and yeast. Background technique [0002] Mold and yeast widely exist in the air, water and soil. Most of them are harmless to the human body, but some molds are harmful to the human body. Mold can also cause mildew in food, stimulate the human digestive tract, stomach, etc. Can damage the liver and cause food poisoning. Potato white sugar agar (PDA), Sabouraud's agar medium (Sabouraud's agar), etc. are used for mold yeast. These mediums are cultured for too long and the colonies are too large, which is not conducive to counting the number of bacteria. Contents of the invention [0003] In order to solve the above-mentioned problems, the present invention proposes a color-developing and counting culture medium for mold and yeast. The present invention has short cultivation time, small bacterial colonies, and is beneficial for counti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 金京勋吕少波李苏杨
Owner STONE LAKE PHARMA TECH