Method for producing prodigiosin by coupling resin adsorption in-situ separation with fermentation
A technology for in-situ separation of prodigiosin, applied in the field of biochemistry, can solve the problems of unfavorable large-scale industrial production and long time consumption
Inactive Publication Date: 2014-04-30
JIAXING UNIV
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However, the pure product must be separated by resin chromatography. Although the effect is good, it takes a long time and is not conducive to large-scale industrial production.
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[0031] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the following further describes the present invention in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.
[0032] figure 1 Shows the process of a method for in-situ separation by resin adsorption and coupled fermentation production of prodigiosin according to the present invention, including the following steps:
[0033] S101: Use secondary fermentation to carry out the fermentation culture of Prodigosin: firstly carry out plate activation, secondly carry out seed culture, and finally expand the culture;
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The invention discloses a method for producing prodigiosin by coupling resin adsorption in-situ separation with fermentation. The method comprises the following steps: performing fermentation culture on prodigiosin through two-level fermentation, namely performing flat activation, seed culture and final amplification culture; performing a single factor experiment according to the sample loading quantity, pH of adsorption liquid, adsorption time and adsorption temperature, and measuring the adsorption rate; and performing an orthogonal experiment according to the experimental factor of the orthogonal experiment determined by the single factor experiment. According to the method disclosed by the invention, the screened high-yield prodigiosin strain is used as an original strain, a culture medium optimized by a research team is adopted for shake flask fermentation, and the optimal adsorption resin is screened; through the design of single factor and orthogonal experiment, the prodigiosin adsorption of the resin is observed, and the best adsorption conditions are determined to provide a basis for coupling the fermentation separation of prodigiosin.
Description
Technical field [0001] The invention belongs to the technical field of biochemistry, and particularly relates to a method for in-situ separation by resin adsorption and coupled fermentation to produce prodigiosin. Background technique [0002] Nowadays, there is a great demand for the research of Prodigalin in the market. As a lipopolysaccharide extracted from natural organisms, it has a good effect on anti-cancer and tumor. Moreover, the current domestic market is mostly red pigment extracted from plants. Compared with bacterial production, the plant extraction cycle is longer and the process is complicated, which will affect the quality and yield of red pigment. At the same time, the Serratia in the laboratory has a higher yield. However, the pure product must be separated by resin chromatography. Although the effect is good, it takes a long time and is not conducive to large-scale industrial production. [0003] Therefore, it is a better solution to seek a fast separation meth...
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IPC IPC(8): C12P17/16
Inventor 刘晓侠孙诗清王玉洁尤忠毓
Owner JIAXING UNIV