Preparation method for elaeis guineensis leaf scanning electron microscope sample

Abstract

The invention relates to a preparation method for an elaeis guineensis leaf scanning electron microscope sample. The preparation method comprises the following steps: cutting an elaeis guineensis leaf into small sections of samples; washing; fixing with FAA (Formalin-Acetic acid-Alcohol) stationary liquid; then carrying out gradient dehydration with different ethanol solutions; freeze-drying the samples and trimming; sequentially adhering the samples to a conductive adhesive tape; plating a gold film to obtain a scanning electron microscope sample. According to the preparation method, the operation steps are simple and convenient; the prepared scanning electron microscope sample has a complete structure; when a scanning electron microscope is used for observation, the stereoscopic impression of an image is strong, the leaf surface structure is clear and complete; the cost is low; the observation and research on an elaeis guineensis ultra-microstructure are facilitated; the preparation method has important significance on obtaining an excellent germplasm resource types and cultivating or screening out the germplasm resource types or breeds suitable for growing in China.

Description

technical field [0001] The invention belongs to the technical field of microscopic observation samples, and relates to a preparation method of a scanning electron microscope sample, in particular to a preparation method of an oil palm leaf scanning electron microscope sample. Background technique [0002] Oil palm (Elaeis guineensis Jacq.) is a monocotyledonous perennial woody oil crop belonging to the family Palmaceae. It is the most efficient oil-producing plant in the world, with an average annual oil production of 4.27 tons per hectare, which is 5-6 times that of peanuts. 9 to 10 times that of soybeans, known as the "world oil king". Oil palm, as an emerging tropical crop with great potential, will play an important role in the supply of edible oil, and has broad prospects for development and utilization. Today's oil palm varieties in my country are all imported from abroad, and it is difficult to determine their adaptability in my country. It needs long-term introducti...

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Problems solved by technology

[0003] At present, the research on the anatomical structure of oil palm leaves is mainly made of thin slices by paraffin section for microscopic observation of its anatomical structure. Observation and distinction of the ultrastructure such as stomata distribution on the leaf surface, the size of the stomata, the texture of the leaf surface, and the style of the anticlinal wall of epidermal cells cannot be obtained through paraffin sections, which cannot provide more direct and reliable identification of germplasm resources. , direct and stable evidence is not conducive to the identification and classification of oil palm germplasm resources

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