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Markers and methods for detection of colorectal cancer or esophageal cancer

A marker and technology for esophageal cancer, applied in biochemical equipment and methods, chemical instruments and methods, microbial measurement/testing, etc., can solve problems such as difficult to interpret results, high false positive rate, and time-consuming

Inactive Publication Date: 2016-02-17
TORAY IND INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have disadvantages such as time-consuming determination of cancer, difficulty in interpreting results, and high false positive rate

Method used

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  • Markers and methods for detection of colorectal cancer or esophageal cancer
  • Markers and methods for detection of colorectal cancer or esophageal cancer
  • Markers and methods for detection of colorectal cancer or esophageal cancer

Examples

Experimental program
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Embodiment

[0115] The present invention will be described more specifically below by way of examples. However, the present invention is not limited by this example.

[0116]

[0117] (1) Production of hollow fiber filter

[0118] Bundle 100 polysulfone hollow fibers with a molecular weight cut-off of about 50,000 on the membrane surface, and fix the two ends on the glass tube with an epoxy resin-based encapsulant on the premise that the hollow part of the hollow fiber is not blocked. into micromodules. This miniature module (module A) can be used to remove high molecular weight proteins in serum or plasma, with a diameter of about 7 mm and a length of about 17 cm. A micromodule (module B) for concentrating low-molecular-weight proteins was similarly fabricated using a membrane with a pore size of molecular weight cut-off about 3 k. The micromodule has an inlet connected to the hollow fiber lumen at one end and an outlet at the other end. The inlet and outlet of the hollow fiber mic...

Embodiment 2

[0128] (1) Detection of COTL1 protein in esophageal cancer blood by Western blot

[0129]Plasma samples were obtained from 3 esophageal cancer patients (No. 1-3) and 4 healthy controls (No. 1-4) who obtained informed consent. 100 μL of Affi-Gel blue glue (Bio-Rad) and 50 μL of Protein A-Sepharose (GE Healthcare) were added to 100 μL of each sample, and reacted overnight at 4°C to remove albumin and immunoglobulin in the sample. The sample thus obtained was solubilized and boiled using SDS loading buffer (50mM Tris hydrochloric acid, pH 6.8, 1mMDTT, 5% SDS, 10% glycerol), and subjected to SDS-polyacrylamide gel (16%) electrophoresis, Proteins were then transferred to PVDF membranes. This was reacted with a rabbit anti-COTL1 polyclonal antibody (Proteintech Group) and a peroxidase-labeled secondary antibody (anti-rabbit IgG antibody). Immunoreactive proteins were visualized by sensitizing the X-ray film using SuperSignalWestFemtoMaximumSensitivitySubstrate (Pierce), and the si...

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Abstract

The present invention relates to a method for detecting or inspecting a cancer selected from colorectal cancer and esophageal cancer, which has low invasiveness to a subject and has high detection sensitivity and accuracy, and specifically relates to a method comprising in vitro determination of a cancer derived from a subject. COTL1 protein in body fluid, a method for examining cancer in a subject based on the amount thereof, which is selected from the steps selected from the above-mentioned cancer; and an antibody or fragment thereof which can specifically bind to the protein for diagnosis A kit for cancer selected from colorectal cancer and esophageal cancer.

Description

technical field [0001] The present invention relates to a method for examining colorectal cancer or esophageal cancer by measuring the concentration of COTL1 protein in body fluids as a marker for detection of colorectal cancer or esophageal cancer. [0002] The present invention also relates to a diagnostic kit for colorectal cancer or esophageal cancer comprising a substance capable of binding to the above protein for detecting colorectal cancer or esophageal cancer. Background technique [0003] Colorectal cancer is the cancer with the second most organ divisions, and 9.8% of all cancer patients are colorectal cancer patients. In 2008, 17.3 people per 100,000 people in the world suffered from colorectal cancer, and 8.2 people died of it. In Japan, colorectal cancer is the second most common cancer in all organs, and the number of patients tends to increase with the Westernization of food and lifestyle. [0004] Esophageal cancer was 7.0 per 100,000 people in the world i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68C07K16/18C12Q1/02G01N33/574
CPCC07K16/18C07K16/30C07K16/3046G01N33/57419G01N33/57496
Inventor 小林道元田中祥德高山爱子郑基晚坂井义治冈部宽
Owner TORAY IND INC