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A preparation method of Danshi Tongli Tablet and its application in the preparation of drugs for inhibiting the proliferation of teratoma cell p19 cells

The technology of Danshitongli tablets and teratoma cells is applied to the preparation of Danshitongli tablets, and the application field of preparing drugs for inhibiting the proliferation of mouse teratoma cells P19 cells, which can solve the problem of large dosage, backwardness and many impurities in patients. and other problems to achieve the effect of reducing the dosage

Inactive Publication Date: 2016-05-11
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no gallstone tongli capsule that adopts ultrafine pulverization, CO 2 There are reports on supercritical extraction, microwave technology and macroporous resin adsorption technology. However, the traditional Chinese medicine is directly extracted by powdering and decocted in water. The process is rough and backward, and there are many impurities, which leads to excessive dosage for patients and is inconvenient to take. products in clinical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take money grass 225g, Yinchen 225g, turmeric 90g, tangerine peel 150g, skullcap 45g, frankincense 35g, mirabilite 80g, alum 35g, rhubarb 45g, triangular 45g, gardenia 45g, licorice 25g, myrrh 35g: Ultrafinely pulverized into fine powder with a particle size range of 5-45 μm for use; take frankincense and myrrh, pulverize into fine powder, and add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the volume percentage of the entrainer in the total extraction solvent is 5%, the extraction pressure is 25MPa, the temperature is 40°C, and the CO 2 The flow rate was 2ml / g crude drug·min, and the extraction time was 200min to obtain a supercritical extract, which was used for later use; the extracted frankincense, myrrh residue and other nine medicinal materials were crushed, added with 3.6L of 70% ethanol, and put into a microwave extraction device for extraction. Microwave extraction, extraction power 700W, extraction twice, 8 minutes each time, comb...

Embodiment 2

[0034] Take money grass 225g, Yinchen 225g, turmeric 90g, tangerine peel 150g, skullcap 45g, frankincense 35g, mirabilite 80g, alum 35g, rhubarb 45g, triangular 45g, gardenia 45g, licorice 25g, myrrh 35g: Ultrafinely pulverized into fine powder with a particle size range of 5-45 μm for use; take frankincense and myrrh, pulverize into fine powder, and add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the volume percentage of the entrainer in the total extraction solvent is 4%, the extraction pressure is 30MPa, the temperature is 50°C, and the CO 2 The flow rate was 3ml / g crude drug·min, and the extraction time was 180min to obtain a supercritical extract, which was used for later use; the extracted frankincense, myrrh residue and other nine medicinal materials were pulverized, added with 3.6L of 70% ethanol, and put into a microwave extraction device for extraction. Microwave extraction, extraction power 600W, extraction twice, 8 minutes each time, c...

Embodiment 3

[0037] Take money grass 225g, Yinchen 225g, turmeric 90g, tangerine peel 150g, skullcap 45g, frankincense 35g, mirabilite 80g, alum 35g, rhubarb 45g, triangular 45g, gardenia 45g, licorice 25g, myrrh 35g: Ultrafinely pulverized into fine powder with a particle size range of 5-45 μm for use; take frankincense and myrrh, pulverize into fine powder, and add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 15MPa, the temperature is 30°C, and the CO 2The flow rate was 1ml / g crude drug min, the extraction time was 220min, and the supercritical extract was obtained, which was used for later use; the extracted frankincense, myrrh residue and other nine medicinal materials were pulverized, added 3.6L of 70% ethanol, and put into a microwave extraction device for extraction. Microwave extraction, extraction power 800W, extraction twice, 10 minutes each time, ...

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PUM

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly provides a preparation method and application of a Danshitongli tablet. The preparation method comprises the following steps: by using 225g of Herba Lysimachiae, 225g of oriental wormwood, 90g of radix curcumae, 150g of dried orange peel, 45g of radix scutellariae, 35g of frankincense, 80g of mirabilite, 35 of white alum, 45g of rhubarb, 45g of rhizoma sparganii, 45g of gardenia, 25g of licorice and 35g of myrrh as active pharmaceutical ingredients, carrying out superfine grinding, supercritical extraction, microwave extraction and macroporous resin adsorption so that the hesperidin content is greatly enhanced. The invention also provides application of the Danshitongli tablet in preparing drugs for inhibiting mouse teratoma cell P19 from cell proliferation.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of Danshitongli tablets and its application in the preparation of a drug for inhibiting the proliferation of mouse teratoma cells P19 cells. Background technique [0002] The standard number of Danshitongli Capsules is WS-10324 (ZD-0324)-2002, which is recorded in the Internal Medicine Hepatobiliary Branch of the National Standard Compilation of Chinese Patent Medicines. It is made from 225g of money grass, 225g of Yinchen, 90g of turmeric, 150g of tangerine peel, 45g of scutellaria, 35g of frankincense, 80g of mirabilite, 35g of alum, 45g of rhubarb, 45g of triangular, 45g of gardenia, 25g of licorice, and 35g of myrrh. , has the effect of clearing heat and promoting gallbladder, removing blood stasis and expelling stones. For acute and chronic cholecystitis and gallstones caused by damp-heat in the liver and gallbladder. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9066A61K9/20A61P35/00A61K33/04A61K33/06
Inventor 李国彬杨海英朱德璋
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV