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Identification method for rapidly detecting drug resistance of plants on glyphosate and application of method

An identification method and glyphosate technology are applied in the identification field of rapid detection of plant resistance to glyphosate, which can solve the problems of high cost and the like, and achieve the effects of low cost, quick effect and simple operation.

Inactive Publication Date: 2014-06-04
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The experimental technique of this method is simple, intuitive and effective. It can basically simulate the field situation and is suitable for the detection of drug resistance of most weeds, but it has the disadvantages mentioned above; while the PCR detection method is technical and expensive.

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  • Identification method for rapidly detecting drug resistance of plants on glyphosate and application of method

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Embodiment 1

[0032] Two kinds of goosegrass preserved in the weed room of the Institute of Plant Protection, Guangdong Academy of Agricultural Sciences (Huizhou Yangcun, collected in Yangcun Guava Garden, Huizhou City, Guangdong Province in September 2011; Panyu Dongsheng, collected in Guangzhou City in September 2011 The biotypes (3-4 leaves stage) of goosegrass in Dongsheng Vegetable Farm, Panyu were measured. 64 plants were selected for each biotype, and each plant was transplanted into a plastic cup filled with soil, until it grew normally to 4-5 leaves. stage, select the 2nd or 3rd leaf, and drop 10uL of different doses of glyphosate liquid on both sides of the midrib of the selected leaf by the micro-injection method (1uL per drop, 10 drops in total), and the dripping doses are 1538 , 1845 and 2152.5g a.i. / ha, 16 plants in parallel for each dose, and 16 plants of the two biotypes were retained without glyphosate as a comparison. Three days after the treatment, the Panyu goosegrass bi...

Embodiment 2

[0034]Goosegrass A and B at the 2-3 leaf stage were collected from two places in Shijing, Baiyun District, Guangdong Province (both were collected in September 2011 from the ridges of vegetable fields of different farmers in Shijing, Baiyun District, Guangzhou City), and transplanted into pots indoors. There are 5 plants per pot, and they will grow to 4-5 leaves and wait for testing. Select the second or third leaf, and drop 10 uL of different doses of glyphosate solution to both sides of the midrib of the selected leaf by the micro-injection method (1 uL per drop, 10 drops in total), and the dripping doses are 1538 , 1845 and 2152.5g a.i. / ha, 5 plants were paralleled for each dose, and 5 plants of the two biotypes were retained without glyphosate as a comparison. The results of 7 days after treatment showed that the three doses of goosegrass A in Baiyun District had no symptoms at the sampling sites and plants within the designed dose range, and the symptom level was 0, which...

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Abstract

The invention belongs to the technical field of identification of drug resistance and discloses an identification method for rapidly detecting the drug resistance of plants on glyphosate and an application of the method. The method comprises the following steps: dripping glyphosate liquid medicine on two sides of the midribs of leaves of the plants to be detected by using a micro applicator, continuously performing seed culture, observing sample application parts and the whole plants for 7 days, rating and determining the drug resistance, weighing, calculating and inhibiting the medium dose. According to the method, a sample to be detected is only required to be subjected to micro sample application, the drug resistance can be identified by observation within 7 days, the sample to be detected is directly detected without obvious selected conditions, the result can be identified within 7 days, the method has obvious advantages compared with long period needed by conventional whole-plant detection, the result is consistent with the whole-plant measurement result, and the method has the advantages of convenience, time conservation, labor conservation, space conservation and capacity of directly detecting field samples and can serve as an early resistance monitoring method.

Description

technical field [0001] The invention belongs to the technical field of drug resistance identification, in particular to an identification method and application for rapidly detecting plant resistance to glyphosate. Background technique [0002] Since the 1980s, a large number of herbicides have been applied worldwide, and the development of global drug-resistant weeds has almost risen in a straight line. As of January 2014, 416 biotypes of 222 (129 dicotyledonous and 93 monocotyledonous) weed species have developed resistance to 21 types of chemical herbicides in various cropland systems worldwide. Weed resistance has become a difficult problem in weed management. [0003] Glyphosate is a systemic broad-spectrum herbicide, and its target enzyme is 5-enolpyruvyl-2shikimate-3-phosphate synthase (EPSPS). Competitive inhibition of EPSPS activity in the shikimate pathway leads to excessive accumulation of shikimic acid in plants, blocking the synthesis of phenylalanine, tyrosin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00G01N5/00
Inventor 杨彩宏冯莉田兴山岳茂峰高家东张泰劼崔烨陈国奇
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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