Closed device for circularly extracting crop leaf surface secreta

A technology of cyclic extraction and secretion, applied in the preparation of test samples, etc., can solve the problems of reagent consumption, extraction speed, no improvement, human hazards, etc., and achieve the effect of cheap equipment, time saving and cost saving

Active Publication Date: 2014-06-11
CHINA TOBACCO HENAN IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Deficiencies in the existing extraction methods: 1) Harm to the human body
2) A large amount of reagent consumption or slow extraction speed
At present, the research work on foliage secretions is a hot topic of research by domestic and foreign scholars, but the most basic and simple extraction of foliage secretions has never been improved in domestic and foreign research since the 1990s.

Method used

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  • Closed device for circularly extracting crop leaf surface secreta

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Embodiment Construction

[0013] The present invention will be described in further detail below in conjunction with the accompanying drawings, but the protection scope of the present invention is not limited thereto.

[0014] Such as figure 1 As shown, a closed crop leaf surface secretions circulation extraction device includes a double-mouthed round bottom flask 1 connected in sequence from bottom to top, a separatory funnel 2 with a suction filter 13, a flat bottom funnel 3, a separator A liquid funnel 4, a tail connecting pipe 5 and a condenser pipe 6, and the branch of the double-necked round bottom flask 1 is connected to the outlet of the condenser pipe 6 through joints 7, 8 and glass tubes 9, 10. The lower end of the separating funnel 2 with the suction filter 13 is sealed and connected with the double-mouthed round-bottom flask 1 through the sleeved plug 11; the top of the flat-bottomed funnel 3 is sealed with the separating funnel 4 by setting a glass plate 12 with holes The aperture of the ...

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Abstract

The invention belongs to the technical field of extraction devices, and particularly relates to a closed device for circularly extracting crop leaf surface secreta. The closed device comprises a double-port round-bottom flask, a separating funnel with a suction filtration nozzle, a flat-bottom funnel, a separating funnel, a tail connecting pipe and a condenser pipe which are sequentially connected from bottom to top, wherein a branch port of the double-port round-bottom flask is connected with an outlet of the condenser pipe through a joint and a glass tube. The device disclosed by the invention makes up for the defects that an existing extraction method is harmful to the health of operators and the consumption of volatile organic solvent is great, the extraction speed is increased, and errors of man-made operation are reduced. The device disclosed by the invention is low in cost and simple in structure, and can safely, efficiently and quickly extract the crop leaf surface secreta.

Description

technical field [0001] The invention belongs to the technical field of extracting devices, and in particular relates to a closed-type crop leaf surface secretion circulation extracting device. Background technique [0002] At present, the commonly used method for extracting leaf exudates is as follows: the area of ​​the leaves must be recorded before soaking. When extracting, pour about 1000ml of dichloromethane into a metal tray (clean without water residue), clamp the base of the petiole with tweezers, immerse the whole leaf in the dichloromethane solution for 2 seconds, and lift the leaf out of the solution for 2 seconds. The dichloromethane on the blade is dripped back into the dish, which is counted as one leaching, and each leaf is repeatedly leached 4 times, and the dichloromethane on the blade needs to be completely dripped back into the dish after the last leaching. Transfer all the dichloromethane in the tray to a 2L beaker, add 1ml of internal standard (if requir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 于建春王冬彭玉富杨铁钊孙九哲王根发张迪
Owner CHINA TOBACCO HENAN IND
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