Pre-enrichment liquid medium for Cronobacter cold and hot injury repair

Abstract

The invention relates to a pre-enrichment liquid culture medium for repairing cold and thermal injuries of Cronobacter. The pre-enrichment liquid culture medium comprises peptone, monopotassium phosphate, disodium hydrogen phosphate, sodium chloride, sucrose, sodium pyruvate, magnesium chloride and water. By verifying a repairing effect of the culture medium by virtue of a lot of food-borne pathogenic bacteria, results show that compared with existing pre-enrichment culture media such as BPW (Buffered Peptone Water), EE (Enterobacteria Enrichment Broth), TSB (Trypticase Soy Broth) and the like, the growth effect and repairing effects of physiological and biochemical indexes of the pre-enrichment liquid culture medium are greatly improved, the missing inspection defect of the Cronobacter in food inspection quarantine processes can be overcome, the detection ratio of the Cronobacter can be improved, and the growth and propagation of non-target bacteria such as salmonella and the like can be effectively restrained. The pre-enrichment liquid culture medium is suitable for rapidly repairing the Cronobacter in various foods and has important significances in the prevention and control of the occurrence and propagation of food-borne diseases caused by the food-borne Cronobacter infection.

Description

technical field [0001] The invention belongs to the technical field of food microbiological inspection, and in particular relates to a liquid culture medium for enriching Cronobacter before cold and heat damage repair. Background technique [0002] Cronobacter ( Cronobacter ), is an important food-borne pathogenic bacterium, a gram-negative bacillus belonging to the family Enterobacteriaceae, which can cause meningitis, fatal colitis, and bacteremia in newborn infants. Epidemiological data surveys show that infant formula Milk powder is the main medium of its infection. [0003] During food processing, through high heat, low cold, drying, sterilization and other processing units, it is easy to cause damage to Cronobacter. Therefore, in the process of food microbiological inspection, the existing pre-enrichment culture medium is not effective in repairing the damaged bacteria or contains selective inhibitors, which leads to the failure of the damaged bacteria to grow or gre...

AI Technical Summary

Problems solved by technology

Therefore, in the process of food microbiological inspection, the existing pre-enrichment culture medium is not effective in repairing the damaged bacteria or contains selective inhibitors, which leads to the failure of the damaged bacteria to grow or great changes in physiological and biochemical reactions, resulting in Cronobacter in food. False-negative results in bacterial testing led to food contaminated by Cronobacter entering the consumer market. Once these damaged bacteria enter the human body through food, they can be revived under suitable conditions, causing the prevalence of foodborne diseases caused by Cronobacter. outbreak