Method for evaluating total content of nitrogen in coniferous tree seedling

A conifer and nitrogen content technology, which is applied in the field of large-area nitrogen content assessment of plants, can solve the problems of small conifer seedlings, unreliable measurement results, and poor real-time observation, and achieve the goal of reducing vegetation damage, reducing workload, and accurate investigation Effect

Inactive Publication Date: 2014-06-18
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

However, due to the poor operability and real-time observation of nitrogen measurement experiments in the field, for scientific research projects located in deep mountains and old forests, the time difference between sampling and testing often leads to unreliable measur

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  • Method for evaluating total content of nitrogen in coniferous tree seedling

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Embodiment 1

[0048] 1. Measurement location: Broad-leaved Korean pine forest in Changbai Mountains.

[0049] 2. Specific species of coniferous tree seedlings measured: Korean pine seedlings.

[0050] The total area measured: the sampling area is 10*10m, which is divided into four sub-squares of 5*5m; the large-scale survey is over 1ha.

[0051] 3. Determination methods, including:

[0052] (1) Use a polyphenol analyzer with a small sample size to measure the absorbance of polyphenols and chlorophyll of coniferous Korean pine seedlings, and the nitrogen content of the aboveground part; the following data are the average values ​​of leaves and stems of Korean pine seedlings:

[0053] Polyphenol absorbance: 0.1885±0.04 (mean±standard error);

[0054] Chlorophyll absorbance: 0.425±0.03;

[0055] Nitrogen content in aerial parts: 109.15±6.01mg / g.

[0056] (2) Calculate the nitrogen balance index according to the absorbance of polyphenols and the absorbance of chlorophyll:

[0057] NBI=Chl / ...

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Abstract

The invention discloses a method for evaluating the total content of nitrogen in a coniferous tree seedling. The method comprises the following steps: (1) determining the absorbancy of polyphenol and chlorophyll of the coniferous tree seedling with a small sample size, and determining the content of the nitrogen of a material of the over-ground part of the coniferous tree seedling with the small sample size; (2) obtaining a nitrogen balance index by using the determined absorbancy of the polyphenol and the chlorophyll, calculating the related coefficient of the nitrogen balance index and the content of the nitrogen of the coniferous tree seedling, and building a regression equation of the balance index and the content of the nitrogen of the coniferous tree seedling; and (3) determining the absorbancy of the polyphenol and the chlorophyll of the coniferous tree seedling with the large sample size, and obtaining the total content value of the nitrogen corresponding to the coniferous tree seedling by using the built regression equation. By adopting the method disclosed by the invention, the total content of the nitrogen of the upgraded over-ground part of the coniferous tree seedling can be quickly and accurately determined, the workload for large-area investigation of the total content of the nitrogen of stems and leaves is reduced, the method has the advantages of being accurate to investigate, simple and convenient to carry out, low in cost and the like, and the damage to the vegetation is reduced.

Description

technical field [0001] The invention relates to a method for evaluating plant nitrogen content, in particular to a method for evaluating total nitrogen content of coniferous tree seedlings, and belongs to the field of large-area nitrogen content evaluation of plants. Background technique [0002] Plant polyphenols are a class of polyphenols that widely exist in plants. The content in plants is second only to cellulose, hemicellulose and lignin. They mainly exist in the bark, roots, wood, leaves and fruits of plants. . Plant polyphenols are very abundant in nature. There are more than 600 common plants containing more polyphenols, and the polyphenol content in some coniferous bark reaches 20%-40%. Research on plant polyphenols can be used as a supplement to the research on plant photosynthesis and chlorophyll fluorescence, and can also be used for research on the development and utilization of plant polyphenols. In addition, polyphenols were significantly correlated with le...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 康文赵莘周伟磊李俊清
Owner BEIJING FORESTRY UNIVERSITY
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