Method for evaluating total content of nitrogen in coniferous tree seedling

Abstract

The invention discloses a method for evaluating the total content of nitrogen in a coniferous tree seedling. The method comprises the following steps: (1) determining the absorbancy of polyphenol and chlorophyll of the coniferous tree seedling with a small sample size, and determining the content of the nitrogen of a material of the over-ground part of the coniferous tree seedling with the small sample size; (2) obtaining a nitrogen balance index by using the determined absorbancy of the polyphenol and the chlorophyll, calculating the related coefficient of the nitrogen balance index and the content of the nitrogen of the coniferous tree seedling, and building a regression equation of the balance index and the content of the nitrogen of the coniferous tree seedling; and (3) determining the absorbancy of the polyphenol and the chlorophyll of the coniferous tree seedling with the large sample size, and obtaining the total content value of the nitrogen corresponding to the coniferous tree seedling by using the built regression equation. By adopting the method disclosed by the invention, the total content of the nitrogen of the upgraded over-ground part of the coniferous tree seedling can be quickly and accurately determined, the workload for large-area investigation of the total content of the nitrogen of stems and leaves is reduced, the method has the advantages of being accurate to investigate, simple and convenient to carry out, low in cost and the like, and the damage to the vegetation is reduced.

Description

technical field [0001] The invention relates to a method for evaluating plant nitrogen content, in particular to a method for evaluating total nitrogen content of coniferous tree seedlings, and belongs to the field of large-area nitrogen content evaluation of plants. Background technique [0002] Plant polyphenols are a class of polyphenols that widely exist in plants. The content in plants is second only to cellulose, hemicellulose and lignin. They mainly exist in the bark, roots, wood, leaves and fruits of plants. . Plant polyphenols are very abundant in nature. There are more than 600 common plants containing more polyphenols, and the polyphenol content in some coniferous bark reaches 20%-40%. Research on plant polyphenols can be used as a supplement to the research on plant photosynthesis and chlorophyll fluorescence, and can also be used for research on the development and utilization of plant polyphenols. In addition, polyphenols were significantly correlated with le...

AI Technical Summary

Problems solved by technology

However, due to the poor operability and real-time observation of nitrogen measurement experiments in the field, for scientific research projects located in deep mountains and old forests, the time difference between sampling and testing often leads to unreliable measurement results; Smaller, it is more difficult to collect stems and leaves and analyze the nitrogen content of stems and leaves. In addition, there is a certain time lag in the determination of nitrogen content by Kjeldahl method in areas with inconvenient transportation.

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