A tissue-engineered artificial nerve and its preparation method

A tissue engineering and nerve technology, applied in the field of biomedical engineering, can solve the problems of difficult production and complex microfabrication technology.

Inactive Publication Date: 2016-03-02
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the micro-fabrication technology is complex and difficult to manufacture.

Method used

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  • A tissue-engineered artificial nerve and its preparation method
  • A tissue-engineered artificial nerve and its preparation method
  • A tissue-engineered artificial nerve and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 silk monofilament

[0044] Silk monofilament is the main material used in this study. The quality of the prepared monofilament is directly related to the quality of the artificial nerve. Because the silk is relatively slender, it is more difficult in the experimental operation. In order to facilitate the experimental operation, in this embodiment, first n (100-500) silks are bundled into tows, cut into a certain length, and the ephrinB2 / After the monofilament slow-release carrier is successfully prepared, it is further cut. Follow the steps below:

[0045] 1) Degumming

[0046]First, 100-500 silks are bundled into tows with surgical sutures, and cut into 10-15cm lengths. Place in a beaker, add sodium carbonate aqueous solution (concentration of sodium carbonate aqueous solution is shown in Table 1), place in an autoclave for degumming (degumming treatment pressure, temperature and degumming treatment time parameters are shown in Tabl...

Embodiment 2

[0053] The preparation of embodiment 2 ephrinB2 monofilament sustained-release carrier

[0054] The ephrinB2 silk monofilament slow-release carrier was prepared from the obtained degummed silk monofilament prepared under the experimental conditions of No. 2 group in Example 1. The specific operation is carried out according to the following steps:

[0055] 1) Soak the degummed silk monofilament tow in ephrinB2 aqueous solution, and freeze-dry;

[0056] 2) soaking the tow after freeze-drying in step 1) in an aqueous gelatin solution, and freeze-drying;

[0057] 3) Soak the tow after freeze-drying in step 2) in PLGA-chloroform solution, and dry it under low pressure. The experimental parameters of steps 1), 2), and 3) are shown in Table 2. The production process of EphrinB2 monofilament (filament) sustained-release carrier is as follows: image 3 shown.

[0058] A certain weight of ephrinB2 monofilament slow-release carrier was taken, crushed, placed in triple distilled wat...

Embodiment 3

[0063] The assembly of embodiment 3 artificial nerves

[0064] The ephrinB2 silk monofilament slow-release carrier obtained in Example 2 was assembled according to the following two schemes respectively:

[0065] Scheme 1 (artificial nerve composed of a single channel): n ten thousand ephrinB2 silk monofilament slow-release carriers 2 are directly placed in the PLGA hollow catheter 1 as the artificial nerve (such as Figure 5 shown in A);

[0066] Scheme 2 (artificial nerve composed of wide-channel units): first insert n pieces of ephrinB2 silk monofilament slow-release carriers 2 into the PLGA hollow microcatheter 3 to form a wide-channel unit, and the wide-channel unit is then used as the structural unit of the artificial nerve. These wide-channel units are then assembled into PLGA hollow conduits 1 as artificial nerves (such as Figure 5 shown in B).

[0067] During the process of arranging the filamentous carriers in the PLGA hollow conduit or in the PLGA hollow microcond...

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Abstract

The invention relates to a tissue engineered artificial nerve and a preparation method thereof. The tissue engineered artificial nerve comprises a PLGA (poly lactic-co-glycolic acid) hollow duct, degummed silk monofilaments and cell factors EphrinB2, wherein the degummed silk monofilaments and the cell factors EphrinB2 form controlled-release carriers which are regularly arranged in the PLGA hollow duct in parallel along a long axis. The preparation method comprises the steps of firstly degumming silks so as to prepare the monofilaments; by using the monofilaments as materials for preparing EphrinB2 filiform controlled-release carriers, then assembling the filiform controlled-release carriers in the PLGA hollow duct so as to obtain the artificial nerve. The artificial nerve has the advantages that the filiform controlled-release carriers are piled up in the artificial nerve so as to form a series of micro-channels among the filiform controlled-release carriers, and the micro-channels compel the ordered growth of axons along the long axis of the tissue engineered artificial nerve in a peripheral nerve defect repair process through the sustained release of the cell factors EphrinB2; the preparation method of the tissue engineered artificial nerve is free of special requirements of a micro-preparation technology for equipment and processes, and has the advantages of being simple, practicable, low in cost and high in quality.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a tissue-engineered artificial nerve and a preparation method thereof. Background technique [0002] Peripheral nerve injury is a common trauma that can occur alone or in combination with other tissue injuries. Peripheral nerve injury caused by trauma or surgery is a common disease in reconstructive surgery. For nerve ruptures, surgical suturing can be performed directly. For longer distances, a graft must be "bridged" between the stumps. For this, a large number of nerve grafts are clinically required. At present, autologous nerve transplantation can achieve the best repair effect, but the source is limited and can cause dysfunction in the donor site; besides these problems, allogeneic nerve transplantation also faces problems such as rejection; this limits the application of autologous and allogeneic nerve transplantation , can not meet the clinical needs. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/54A61L27/56A61L27/58
Inventor 刘彬张耀光宋羽葳游华建陈文琦马燕
Owner SOUTHWEST UNIV
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