A biofilm-forming Brevundimonas diminuta and its application in the treatment of cyanide-containing wastewater
A technology of brevundimonas and biofilm, applied in the field of bioremediation, can solve the problems of weak biofilm ability, achieve the effect of reducing COD value of effluent, enriching diversity and improving removal efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0022] Example 1. Isolation, purification and identification of Brevundimonas diminuta N5
[0023] (1) Isolation and purification of Brevundimonas diminuta N5
[0024] Brevundimonas diminuta (Brevundimonas diminuta) N5 provided by the present invention is separated from the biofilm cultivated by acrylic fiber wastewater and sludge, and acrylic fiber wastewater and sludge samples are collected from a certain acrylic fiber wastewater treatment plant in Daqing, and its specific separation and purification process is as follows:
[0025] Using MBBR (MovingBedBiofilmReactor) biofilm reactor, the sludge and wastewater samples were cultured with biofilm. The reactor is made of transparent polystyrene material with an effective volume of 10L, and an industrial biofilm carrier and glass slide carrier made of polyethylene material are added to the reactor. Sludge samples and acrylic fiber wastewater were added to the reactor, water was fed from the bottom and drained from the upper par...
example 2
[0041] Determination of Example 2 Brevundimonas diminuta (Brevundimonas diminuta) N5 film-forming conditions
[0042] When film-forming bacteria N5 re-formed biofilm, the demand for external organic carbon sources was examined, and different external carbon sources were selected and their concentration conditions were set to verify whether the amount of biofilm formation was increased. The test process is as follows. Glucose, sucrose, lactose, maltose, and glycerol were used as additional carbon sources to prepare TSB culture solutions containing different carbon sources (the initial concentration of different carbon sources was 0.1%), and each well of the 96-well cell culture plate Add 200 μL of each of the above-mentioned culture media in sequence, inoculate the bacterial suspension in each well with 1% inoculum, and culture in a constant temperature incubator at 30°C for 24 hours to quantitatively detect the biofilm. For each experiment, 3 wells were set in parallel, and re...
example 3
[0046] Example 3 Brevundimonas diminuta (Brevundimonas diminuta) N5 is applied to the treatment of cyanide-containing wastewater
[0047] (1) Investigation on the tolerance and utilization of Brevundimonas diminuta N5 to acetonitrile
[0048] Inoculate 50 μL of the bacterial suspension of biofilm-forming bacteria N5 in 5 mL containing 800 mg·L -1 In the synthetic waste water of acetonitrile, inoculate the synthetic waste water without adding acetonitrile as a control, shake and culture at 35°C 180rpm for 24 hours, measure the absorbance value at 600nm, and examine the growth status of biofilm-forming bacteria N5; inoculate 50 μL of biofilm-forming bacteria N5 The bacterial suspension contains 800mg·L in 5mL -1 In the inorganic salt basic medium of acetonitrile, the culture medium without inoculation was used as the control, and the culture medium was shaken at 35°C and 180rpm for 24 hours, and the absorbance value at 600nm was measured to detect whether the biofilm-forming ba...
PUM
| Property | Measurement | Unit |
|---|---|---|
| absorbance | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More