Molecular internal standard substance for identifying authenticity of objects and application of molecular internal standard substance

Abstract

The invention provides a molecular internal standard substance for identifying the authenticity of objects and an application of the molecular internal standard substance. Two nucleotide sequences are designed so as to serve as the molecular internal standard substance which is directly added to liquid food or other goods; a specific amplification and detection method is used for detecting whether the internal standard substance exists so as to judge the authenticity of the liquid food or goods. The molecular internal standard substance has the advantages that nucleic acid molecules with certain sequences are designed so as to serve as the internal standard substance for identifying the authenticity of the goods, primers do not need to be additionally added in a detection identifying process, the trouble caused by using other methods for designing and amplifying the primers is avoided, the molecular internal standard substance is free of interference of nucleic acid molecules contained in food and has strong accuracy and strong specificity, particularly the reaction condition is constant temperature, and the complicated operations such as temperature control are reduced.

Description

technical field [0001] The invention belongs to the technical field of anti-counterfeiting identification, and in particular relates to a molecular internal standard used for identifying the authenticity of articles and its application. Background technique [0002] For the current food, especially the expensive liquor and health products, the current anti-counterfeiting measures mainly start with the outer packaging. Outer packaging anti-counterfeiting technology mainly includes two categories: logo anti-counterfeiting and packaging structure anti-counterfeiting: logo anti-counterfeiting mainly uses high-tech technology to attach various logos to the outer packaging for easy identification by consumers; packaging structure anti-counterfeiting starts with the structure of the packaging, common There is a one-time break package. However, the outer packaging is easy to be counterfeited, and the markings on the outer packaging have disadvantages such as being easily scratched,...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a molecular internal standard for identifying the authenticity of articles and its application, aiming to solve the problem of traditional PCR amplification technology being interfered by nucleic acid molecules contained in food, false positive results, and amplification problems. Adding troublesome problems in primer design

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