A preparation method of yeast magnetic imprinted composite microsphere adsorbent
A technology of composite microspheres and yeasts, applied in chemical instruments and methods, alkali metal compounds, alkali metal oxides/hydroxides, etc., can solve problems such as endocrine disorders and central nervous palsy, and achieve good magnetic strength and fast Effect of Separation, Excellent Choice Recognition Performance
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Embodiment 1
[0042] 1. A method for preparing yeast magnetically imprinted composite microsphere adsorbent, characterized in that it is carried out according to the following steps:
[0043] (1) Fe 3 o 4 Preparation of nanoparticles and their hydrophobic modification:
[0044] 1gFeCl 3 ·6H 2 O and 0.5gFeCl 2 4H 2 O was dissolved in 50ml of deionized water, stirred at 20°C for 10min, 30ml of NaOH solution (concentration: 0.2mol / L) was quickly added to the above solution in a nitrogen atmosphere, and vigorously stirred for 10min. Generated Fe 3 o 4 After the nanoparticles were separated and recovered by a magnet, they were washed 3 times with absolute ethanol, dispersed in 40 ml of a mixture of oleic acid and absolute ethanol with a volume ratio of 1:1, and stirred for 3 h at 30°C. Washed 6 times with water and ethanol, and dried under vacuum at 40°C.
[0045] (2) Modification of yeast:
[0046] Disperse 0.5g of yeast in 30ml of a mixed solution of water and absolute ethanol with ...
Embodiment 2
[0060] 1. A method for preparing yeast magnetically imprinted composite microsphere adsorbent, characterized in that it is carried out according to the following steps:
[0061] (1) Fe 3 o 4 Preparation of nanoparticles and their hydrophobic modification:
[0062] 3gFeCl 3 ·6H 2 O and 1.5gFeCl 2 4H 2 O was dissolved in 100ml of deionized water, stirred at 50°C for 20min, 60ml of NaOH solution (concentration: 0.6mol / L) was quickly added to the above solution in a nitrogen atmosphere, and vigorously stirred for 30min. Generated Fe 3o 4 After the nanoparticles were separated and recovered by a magnet, they were washed 3 times with absolute ethanol, dispersed in 40ml of a mixture of oleic acid and absolute ethanol with a volume ratio of 1:4, and stirred at 70°C for 8 hours. Washed 6 times with water and ethanol, and dried under vacuum at 40°C.
[0063] (2) Modification of yeast:
[0064] Disperse 2.5g of yeast in 30ml of a mixed solution of water and absolute ethanol wi...
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