Method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx
A technology of steroidal saponins and brocade lanterns, applied in the field of drug extraction, can solve the problems of high energy consumption, cumbersome, time-consuming operation and the like
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Embodiment 1
[0018] (1) Dried brocade lantern stems in an oven at 100-105°C, crushed into powder with a pulverizer, passed through a 40-80 mesh sieve, and sealed in a dry ziplock bag for later use;
[0019] (2) Establishment of the standard curve: Weigh 5-10mg of diosgenin in a 50ml volumetric flask and make a standard solution of 0.05-0.2mg / ml with anhydrous methanol. Take the standard solution and add vanillin-perchloric acid to develop the color and then measure the wavelength. Scanning, the maximum wavelength is 500-560nm. Pipette 300, 400, 500, 1100, 1300, 1500ul into stoppered test tubes respectively, after evaporating in a water bath, add 0.1-0.5ml (2-5%) vanillin-glacial acetic acid solution, 0.5-1.0ml high chlorine Acid, 65 ~ 70 ℃ water bath, heating 10 ~ 15min, ice water cooling; add 4.0 ~ 6.0ml of glacial acetic acid and let it stand for 10 ~ 30min, measure the absorbance at 560nm. Take the absorbance (A) as the ordinate Y, and the concentration C of dioscin as the abscissa X t...
Embodiment 2
[0024] ⑴The calyx of Jindenglantern is dried in an oven at 100-105°C, crushed into powder with a pulverizer, passed through a 40-80 mesh sieve, and sealed in a dry ziplock bag for use;
[0025] (2) Establishment of a standard curve: same as (2) in Example 1.
[0026] (3) Accurately weigh three parts of 0.1-0.5g calyx calyx powder, extract with 60-90% ethanol solution for 5-15 minutes, extract 1-5 times, and the ratio of solid to liquid is 1:10-1:40. Extract under the condition of 380~680W;
[0027] ⑷ After the extraction is completed, filter with suction, concentrate by rotary evaporation, and remove to obtain the extract for use;
[0028] (5) Determine the content of steroidal saponins by the vanillin-perchloric acid method, and dilute the extract after rotary steaming to a 100ml volumetric flask; pipette 0.1ml into a 10ml stoppered test tube, and evaporate the solvent to dryness in a 60°C water bath; Then add 0.1 ~ 0.5ml (2 ~ 5%) vanillin-glacial acetic acid solution, 0.5 ...
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