Method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx
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A technology of steroidal saponins and brocade lanterns, applied in the field of drug extraction, can solve the problems of high energy consumption, cumbersome, time-consuming operation and the like
Active Publication Date: 2014-07-23
SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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Problems solved by technology
In the past, the extraction of steroidal saponins in Jindenglong mainly used the water bath reflux extraction
Method used
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Embodiment 1
[0018] (1) Dried brocade lantern stems in an oven at 100-105°C, crushed into powder with a pulverizer, passed through a 40-80 mesh sieve, and sealed in a dry ziplock bag for later use;
[0019] (2) Establishment of the standard curve: Weigh 5-10mg of diosgenin in a 50ml volumetric flask and make a standard solution of 0.05-0.2mg / ml with anhydrous methanol. Take the standard solution and add vanillin-perchloric acid to develop the color and then measure the wavelength. Scanning, the maximum wavelength is 500-560nm. Pipette 300, 400, 500, 1100, 1300, 1500ul into stoppered test tubes respectively, after evaporating in a water bath, add 0.1-0.5ml (2-5%) vanillin-glacial acetic acid solution, 0.5-1.0ml high chlorine Acid, 65 ~ 70 ℃ water bath, heating 10 ~ 15min, ice water cooling; add 4.0 ~ 6.0ml of glacial acetic acid and let it stand for 10 ~ 30min, measure the absorbance at 560nm. Take the absorbance (A) as the ordinate Y, and the concentration C of dioscin as the abscissa X t...
Embodiment 2
[0024] ⑴The calyx of Jindenglantern is dried in an oven at 100-105°C, crushed into powder with a pulverizer, passed through a 40-80 mesh sieve, and sealed in a dry ziplock bag for use;
[0025] (2) Establishment of a standard curve: same as (2) in Example 1.
[0026] (3) Accurately weigh three parts of 0.1-0.5g calyx calyx powder, extract with 60-90% ethanol solution for 5-15 minutes, extract 1-5 times, and the ratio of solid to liquid is 1:10-1:40. Extract under the condition of 380~680W;
[0027] ⑷ After the extraction is completed, filter with suction, concentrate by rotary evaporation, and remove to obtain the extract for use;
[0028] (5) Determine the content of steroidal saponins by the vanillin-perchloric acid method, and dilute the extract after rotary steaming to a 100ml volumetric flask; pipette 0.1ml into a 10ml stoppered test tube, and evaporate the solvent to dryness in a 60°C water bath; Then add 0.1 ~ 0.5ml (2 ~ 5%) vanillin-glacial acetic acid solution, 0.5 ...
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Abstract
The invention relates to a method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx, relating to a drug extracting method, which comprises the following steps of crushing dried Chinese lantern-plant stems and persistent calyx, then extracting for 1-5 times and 5-15 min with the microwave radiation power of 380-680 W and 60-90% ethanol, wherein the material-to-liquid ratio is 1:10 to 1:40; after extracting, filtering, extracting, mixing extraction solutions to prepare an extract; after the extract is dissolved, measuring through a vanillic aldehyde-perchloric acid colorimetric method based on dioscin as the standard substance to calculate the content of saponin in the extract. By means of the microwave extraction method, the extraction time can be obviously shortened; the yield of steroid saponin is increased; the medicinal and edible dual-purpose resources of steroid saponin in the Chinese lantern-plant stems can be sufficiently utilized; in addition, after being separated and purified, steroid saponin in Chinese lantern-plant stems and persistent calyx extracted through the method disclosed by the invention can be used for preparing the drugs, such as antibacterial drugs, expectorant drugs, antiplatelet drugs, lipid-lowering drugs and anti-tumour drugs, or used as the raw material of functional foods and health-care foods.
Description
technical field [0001] The invention relates to a method for extracting medicines, in particular to a method for extracting steroidal saponins from stems and persistent calyxes. Background technique [0002] brocade lantern ( Physalis alkekengi ), alias Physalis, Hanging Lantern, Red Girl, etc., is a plant of the Solanaceae Physalis genus, the fruit is a spherical berry is a perennial herb, and its fruit is edible. It is a health-care fruit with a long history in my country. It is also recorded in the past dynasties of Materia Medica. It is mainly produced in the three eastern provinces, Hebei, Xinjiang, Shandong and other places. The main components of brocade lanterns are steroids, sterols, alkaloids, amino acids, volatile oils, polysaccharides, inorganic elements and so on. Pharmacological studies have shown that brocade lanterns have good pharmacological effects such as anti-inflammatory, anti-malarial, anti-cancer, and antibacterial. According to the basic struc...
Claims
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