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A nematode real-time automatic tracking imaging system

An imaging system and automatic tracking technology, applied in the direction of using feedback control, etc., can solve the problems of dependence on transcription reagents and light dose, inapplicable approach and avoidance behavior, and inability to accurately describe the compound function of target neurons

Active Publication Date: 2016-10-12
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the results of behavioral studies based on nematode mutants are still unable to accurately characterize the complex functions of target neurons, electrophysiological studies and imaging studies based on fixed nematodes are not suitable for approaching and avoiding behaviors, behavioral studies based on laser ablation or optogenetics Scientific research is extremely dependent on transcription reagents and light dose

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  • A nematode real-time automatic tracking imaging system
  • A nematode real-time automatic tracking imaging system
  • A nematode real-time automatic tracking imaging system

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Experimental program
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Embodiment approach 1

[0037] Implementation Mode 1: Objective Function Optimization Method

[0038] After obtaining the recognized binarized nematode image, the form of the selected objective function is as follows,

[0039] E = ∫ 0 1 { E int ( v ( s ) ) + E d i s t ( v ( s ) ) } d s

[0040] Among them, v(s), s∈[0,1] represents the parametric equation of the nematode central curve. E in the above formula int Describes the continuous smoothness of the parametric curve; E dist The minimum value of is obtained at the centerline of the nematode, and describes the degree of fitti...

Embodiment approach 2

[0044] Implementation Mode 2: Shortest Distance Distribution Extremum Search Method

[0045] After obtaining the recognized binarized nematode image, first calculate the shortest distance values ​​from all internal points of the nematode to its exterior in the image to obtain the shortest distance matrix. It can be seen from the analysis that the shortest distance value of the point on the center line reaches a maximum value in the width direction, and this feature can be extracted by acting on the Laplacian operator on the shortest distance matrix of the point on the center line, specifically as follows: After the Laplace operator, the value at the point on the center line is basically less than -0.25, while the values ​​at the other points are all around 0. This method cooperates with other methods, such as removing edge points, adding the median filter value of the current point, etc., to extract the discrete point series on the nematode centerline well.

[0046] In the ab...

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Abstract

The invention provides a nematode real-time automatic tracking imaging system which comprises a light source device, a four-axis loading motion device, an image acquisition device and a main control device. The light source device provides bright field illumination for nematodes. The four-axis loading motion device comprises a two-axis translation platform and a rotating platform, and is used for adjusting the positions of the nematodes in a vertical axis plane according to control instructions of the main control device and making a region of interest of the nematodes always located at the center of an acquisition region of the image acquisition device. The image acquisition device is used for acquiring original images of the moving nematodes in real time. The main control device is used for conducting calculation according to the original images, acquired by the image acquisition image, of the nematodes, determining a discrete point range on a nematode central line, fitting a central curve of the nematodes according to the central line point range, determining the position of a current frame of the central point of the region of interest of the nematodes according to coordinates of the central curve, predicting the position of a next frame of the central point of the region of interest by the combination of the prediction position of a former frame and the measurement position of the current frame of the central point of the region of interest, and sending the control instructions to the four-axis loading motion device. By the adoption of the nematode real-time automatic tracking imaging system, real-time tracking imaging can be accurately conducted on the region of interest of the living body nematodes which freely creep without restriction.

Description

technical field [0001] The invention relates to an imaging device, in particular to a real-time automatic tracking imaging system for nematodes. Background technique [0002] At present, domestic and foreign studies on nematode sensory behavior mainly adopt the following three methods: one is to study nematode mutants; the other is to fix nematodes for electrophysiological recording or fluorescence imaging; Specific neurons, thereby ascertaining the function of specific neurons. Among them, the results of behavioral studies based on nematode mutants are still unable to accurately characterize the complex functions of target neurons, electrophysiological studies and imaging studies based on fixed nematodes are not suitable for approaching and avoiding behaviors, behavioral studies based on laser ablation or optogenetics Scientific research is extremely dependent on transcription reagents and light dose. Contents of the invention [0003] The object of the present inventio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G05D3/12
Inventor 毛珩陶乐天乔晗李宣成姜明
Owner PEKING UNIV