Preparation and application of an anammox bacteria immobilized straight cylindrical bioactive filler based on mesh carrier
A technology of anaerobic ammonium oxidation and biological activity, which is applied in the field of preparation of straight cylindrical bioactive fillers immobilized by anammox bacteria, can solve the problems of easy detachment of embedded bodies, poor overall stability, and unsuitable application, and achieve overall good , not easy to fall off, and the effect of increasing the bacterial density
Active Publication Date: 2016-04-27
BEIJING UNIV OF TECH
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AI Technical Summary
Problems solved by technology
This method is simple to operate, but has great limitations and cannot be ideally applied to engineering practice
Experimental studies have shown that the bioactive fillers prepared by combining traditional carriers such as Raschig rings, Pall rings, stepped rings, and suspended modified fillers with embedding bodies through adhesion have poor overall stability and poor embedding. Body easy to fall off, bacteria loss and other problems
Method used
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[0033] The present invention will be further specifically described below in conjunction with examples, but the embodiments of the present invention are not limited thereto.
[0034] 1. Preparation of Mesh Support
[0035]Polypropylene polymer is used as the main material, and the hydrophilic material polyvinyl alcohol (PVA) with a mass fraction of 5% is added to make a straight cylindrical mesh with a diameter of 0.8cm, a height of 1.0cm, and a wire diameter of 1.0mm. The carrier has a mesh shape of a circle with a diameter of 2 mm.
[0036] 2. Preparation of Anammox Bacteria Concentrate
[0037] The cultured anammox bacterial suspension was centrifuged and concentrated to obtain a bacterial concentration of 2.5×10 10 A / mL concentration of anammox bacteria.
[0038] 3. Acquisition of Anammox Bacteria Embedding Solution
[0039] Weigh 270.0g of polyvinyl alcohol and add it to 2100ml of water, heat and dissolve it at 100°C in a high-temperature sterilizer for 15 minutes, ta...
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Abstract
The invention discloses preparation and an application of an anaerobic ammonia oxidizing bacteria immobilized straight-tubular bioactive filler based on a reticulate carrier, and belongs to the technical field of water treatment. The straight-tubular bioactive filler comprises two parts of an embedding body and the carrier, wherein the embedding body is formed by boric acid cross-linking immobilization of an embedding liquid; the carrier is a reticulate straight-tubular carrier formed by taking polyethylene or polypropylene as a main material, adding a hydrophilic material (polyvinyl alcohol) and carrying out hot melting or plate hot pressing; with a reticulate structure of the carrier, the embedding body can be allowed to penetrate through meshes to form a riveting fixing structure so as to increase the filler overall stability; the embedding liquid is formed by mixing an anaerobic ammonia oxidizing bacteria suspension and a polyvinyl alcohol solution; the embedding liquid evenly coats the reticulate carrier, then is subjected to boric acid secondary cross-linking immobilization and is combined onto the reticulate carrier to obtain the anaerobic ammonia oxidizing bacteria bioactive filler. The prepared bioactive filler not only can effectively solve the problems that the bacteria embedding body easily falls off, is easy to lose and the like, but also can avoid the influence of generated nitrogen gas on mass transfer and shorten the start-up time of a reactor.
Description
technical field [0001] The invention belongs to the field of water treatment, and in particular relates to the preparation and application of an anammox bacteria-immobilized straight cylindrical bioactive filler based on a mesh carrier. Background technique [0002] Anaerobic ammonium oxidation technology (ANAMMOX) is the most economical biological denitrification method known at present. The research of Strous et al., Delft University in the Netherlands shows that the biological reaction process including cell synthesis in the anaerobic ammonium oxidation process can be expressed as the formula ( 1) [0003] NH 4 + +1.32NO 2 - +0.066HCO 3 - +0.13H + → [0004] 1.02N 2 +0.26NO 3 - +0.066CH 2 o 0.5 N 0.5 +2.03H 2 O(1) [0005] From the biological reaction characteristics of ANAMMOX, it can be seen that compared with the traditional nitrification and denitrification technology, ANAMMOX has the advantages of low oxygen demand, low operating cost and no additiona...
Claims
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IPC IPC(8): C12N11/08C02F3/28C02F3/34
Inventor 杨宏王猛管清坤陈伟鄢琳王小乐吴城锋孟婷陶慕翔尚海源胡希佳赵月兰王玉洁姚仁达
Owner BEIJING UNIV OF TECH