Method for preparing protoplast of Eucheuma denticulatum from extract of internal organs of Siganus Concatenalu

Abstract

The invention discloses a method for preparing protoplast of Eucheuma denticulatum from the extract of internal organs of Siganus Concatenalu. The method comprises the following steps: (1) feeding Siganus Concatenalu with Eucheuma denticulatum; (2) acquiring crude enzyme extract from the internal organs of Siganus Concatenalu; and (3) subjecting Eucheuma denticulatum to enzymatic hydrolysis with the crude enzyme extract so as to obtain the protoplast. The method provided by the invention uses easily available raw materials and has complete and stable results; with the method, the protoplast with a complete structure and without cell walls can be obtained, and a good base is laid for subsequent germplasm seedling cultivation through protoplast healing and tissue culture.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing protoplasts. Background technique [0002] Eucheuma serrata is an important ι-carrageenan-producing red algae with broad market development and application prospects. In my country, Eucheuma fine-toothed is except producing iota carrageenan and is used in food and pharmaceutical processing and manufacturing, and can also be directly used to be processed into cold and dressed with sauce instant food products, and only one item of cold and dressed with sauce instant food products consumes about 2000 tons of dry products every year in my country. [0003] Internationally, the cultivation of Eucheuma serrata is mostly carried out in tropical and subtropical regions, and Eucheuma serrata is mainly produced in Southeast Asian countries and regions such as Indonesia and the Philippines. In the past, the large-scale cultivation of this algae mainly relied on vegetativ...

AI Technical Summary

Problems solved by technology

In the past, the large-scale cultivation of this algae mainly relied on vegetative reproduction, but the spore reproduction and sexual reproduction methods of this algae have been seriously degraded, resulting in germplasm degradation, slow growth, reduced rubber production performance, and reduced disease resistance.

[0004] So far, there has been no major breakthrough in the cultivation and breeding of Eucheuma serrata at home and abroad.

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