Preparation method of Isaria javanicus conidium microcapsules

A technology of Corynebacterium javanica and conidia, which is applied in the field of pest biological control and achieves the effects of important popularization and application value, low cost of raw materials and easy operation.

Active Publication Date: 2014-08-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no microcapsule preparation with high embedding rate and excellent insecticidal effect against Corynespora javanica

Method used

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  • Preparation method of Isaria javanicus conidium microcapsules
  • Preparation method of Isaria javanicus conidium microcapsules
  • Preparation method of Isaria javanicus conidium microcapsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of 10% (pure pollen / composite glue W / W) conidia microcapsules of Corynespora javanica

[0044] figure 1 Be the preparation flowchart of Corynespora Javanese conidia microcapsules, specifically comprise the following steps:

[0045] S1. Preparation of cystic core: Take 1 g of pure sporopollen of Corynespora javanica SP053 strain, add 10 mL of 0.05% Tween 80 solution, vortex and stir, prepare bacterial suspension, and set aside.

[0046] S2. Preparation of capsule wall: take 5 g of gelatin, add 35 mL of distilled water to soak and swell, and heat to dissolve into a solution; take 5 g of gum arabic, add 35 mL of distilled water and heat to about 80°C to dissolve into a solution; keep both solutions at 40°C for later use .

[0047] S3. Preparation of microcapsules: under the condition of 40°C water bath, mix the gelatin solution and the capsule core material, and stir gently evenly. After 5 minutes, add gum arabic solution with the same qua...

Embodiment 2

[0048] Example 2 Preparation of 5% (pure pollen / composite glue W / W) conidia microcapsules of Corynespora javanica

[0049] figure 1 Be the preparation flowchart of Corynespora Javanese conidia microcapsules, specifically comprise the following steps:

[0050] S1. Preparation of cystic core: Take 0.5 g of pure sporopollen of Corynespora javanica SP053 strain, add 5 mL of 0.05% Tween 80 solution, vortex and stir, prepare bacterial suspension, and set aside.

[0051] S2. Preparation of capsule wall: take 5 g of gelatin, add 35 mL of distilled water to soak and swell, and heat to dissolve into a solution; take 5 g of gum arabic, add 35 mL of distilled water and heat to about 80°C to dissolve into a solution; keep both solutions at 40°C for later use.

[0052] S3. Preparation of microcapsules: under the condition of 40°C water bath, mix the gelatin solution and the capsule core material, and stir gently evenly. After 5 minutes, add gum arabic solution equivalent to the g...

Embodiment 3

[0062] Example 3 Performance determination of conidia microcapsules

[0063] After sample preparation of the microcapsule dry powder prepared in Example 1 and Example 2, scanning electron microscope observation was carried out, and the results showed that the microcapsules were all spherical in shape, uniform in size, smooth in surface, and had little difference in diameter, with an average particle size of 20- 30 μm ( image 3 ).

[0064] Detection of conidia germination rate: Take 5-10 mg of conidia microcapsules prepared in Example 1 and Example 2, add 100 mL of 0.05% Tween 80 aqueous solution, vibrate to break the wall, and filter through three layers of gauze to obtain The conidia suspension was cultured at 25°C for a certain period of time, and the germination rate was checked. The result is as Figure 4 As shown, after the conidia microcapsules were stored for 180 days, the germination rate of conidia remained above 90%, while the germination rate of conventional c...

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Abstract

The invention relates to the technical field of biological control of insect pests and specifically discloses a preparation method of Isaria javanicus conidium microcapsules. According to the conidium microcapsules, a conidium suspension of Isaria javanicus is used as a capsule core, a complex adhesive formed by equal amounts of gelatin and Arabic gum is used as a capsule wall, transglutaminase is used as a curing agent, and a complex coacervation reaction is carried out to prepare the Isaria javanicus conidium microcapsules. The prepared conidium microcapsules are ball-shaped, have uniform size and have smooth surface, and average particle size is about 20-30 microns. The microcapsules have a long storage period and have high uvioresistant ability. Shelf life of fungi insecticides can be prolonged. Meanwhile, storage stability of pesticides and biological control effects of insect pests are enhanced. The Isaria javanicus conidium microcapsules have practical application value in insect prevention for crops.

Description

technical field [0001] The invention relates to the technical field of pest biological control, in particular to a method for preparing conidia microcapsules of Corynespora javanica. Background technique [0002] At present, the extensive use of chemical pesticides is still the most important method of controlling pests. However, the use of chemical pesticides not only costs a lot, but also pollutes the environment and affects food safety; These shortcomings make people pay more and more attention to the development of pest biological control technology. [0003] Insect-growing fungi are rich in resources, are non-toxic to humans and animals, and do not cause environmental pollution. Therefore, fungal pest control plays an important role in biological pest control. However, because fungal insecticides are living organisms, they are sensitive to environmental factors such as high temperature, drought, and ultraviolet rays. As insecticides, they have a short shelf life and un...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/28A01N63/04A01P7/04B01J13/14
Inventor 胡琼波尹飞钟国华翁群芳董廷艳
Owner SOUTH CHINA AGRI UNIV
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