Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant
A technology for bio-organic fertilizer and livestock and poultry manure is applied in the field of livestock and poultry manure decomposing inoculum and its use method, and the field of the prepared bio-organic fertilizer, and can solve the problems of unimproved income, single inoculum performance and low added value. , to achieve good deodorization performance, reduce the use of chemical fertilizers, and improve crop quality.
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Embodiment 1
[0038] Preparation of media and strains
[0039] 1. Preparation of culture medium
[0040] 1. Potato and dextrose agar medium (PDA)
[0041] Take 200 grams of peeled potatoes, cut them into small pieces, add 1000ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000ml, add 20 grams of glucose, 15 grams of agar, dissolve and subpackage, sterilize for 30 minutes in 15 pounds , the pH is natural, and no agar is added when preparing the liquid medium.
[0042] 2. Nutrient gravy agar
[0043]It consists of 5g of peptone, 5g of sodium chloride, 3g of beef extract and 1000ml of distilled water. pH7.0 (add 15-20g of agar to the solid medium).
[0044] 3. Gao's Synthetic Medium No. 1
[0045] KNO 3 1g, K 2 HPO 4 0.5g, NaCl 0.5g, FeSO 4 7H 2 O 0.01g, MgSO 4 7H 2 O 0.5g soluble starch 20g, pH7.2~7.4, distilled water 1000ml.
[0046] 4. Potassium bacteria medium
[0047] Yeast Extract 0.4g, Mannitol 10.0g, K 2 HPO 4 0.5g, MgSO ...
Embodiment 2
[0066] Composition of compound bacterial agent
[0067] A livestock and poultry manure decomposing bacterial agent, prepared as follows (by weight):
[0068] Table 1 Composition of livestock and poultry manure decomposing bacteria agent
[0069] name Bacillus subtilis Streptomyces flavinus Long handle Trichoderma Bacillus peptiformis (1) 0.5 2.0 1.0 2.0 (2) 1.0 1.5 2.0 1.5 (3) 1.5 1.0 1.5 1.0 (4) 2.0 0.5 0.5 0.5
[0070] The number of effective viable bacteria is 6×10 8 piece / g;
[0071] Table 2 Proportion of raw materials for bio-organic fertilizer production (by weight percentage)
[0072] name Chicken manure (%) Cow dung (%) Pig manure (%) lignite(%) (1) 40 40 10 10 (2) 45 30 15 10 (3) 50 30 5 15 (4) 60 20 15 5
Embodiment 3
[0074] Mutual antagonism experiment between strains
[0075] The activated Bacillus subtilis, Streptomyces flavinus, Trichoderma longiflorum and Bacillus peptone-like were subjected to the mutual antagonism experiment among the strains, and they were combined in pairs, divided into 12 combinations in total, and each combination was repeated three times.
[0076] The steel ring method was used for the antibacterial test, and the strain was cultured in the corresponding medium for 3-7 days (190r / min, 28°C) to make a fermentation broth. Take 0.1mL of the fermentation broth of one of the bacteria (A) and spread it on a PDA plate, then place two pre-sterilized steel circles on the solid medium of each plate, and then add 0.25mL to each steel circle For the fermentation broth of any bacteria (B) other than bacteria A, each treatment was repeated three times, and the plate only inoculated with bacteria A was used as a control. Incubate at 28°C and observe the antibacterial situati...
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