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Feed microecological preparation and preparation method thereof

A microbial preparation and feeding technology, which is applied in the field of feeding microbial preparations and their preparation, can solve the problems of poor stability and repeatability of the effect, and the application effect is not outstanding, so as to achieve strong palatability, increase daily weight gain, and improve The effect of feed conversion ratio

Inactive Publication Date: 2016-02-24
凯帕雷森(江苏)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many enterprises in my country have realized the industrialized production of live microbial preparations for feed and applied them in feed, but there are problems such as not outstanding application effect, poor effect stability and repeatability.

Method used

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  • Feed microecological preparation and preparation method thereof
  • Feed microecological preparation and preparation method thereof
  • Feed microecological preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 liquid preparation:

[0021] 1. Source of strain

[0022] Lactobacillus acidophilus source: Institute of Microbiology, Chinese Academy of Sciences Culture Collection Management Center CGMCC, Lactobacillus plantarum subsp., AS1.2707; Bacillus subtilis Source: Institute of Microbiology, Chinese Academy of Sciences Culture Collection Management Center CGMCC, Bacillus subtilis Subspecies, AS1.2163; Source of Saccharomyces cerevisiae: CGMCC, Saccharomyces cerevisiae, AS2.3875, Institute of Microbiology, Chinese Academy of Sciences.

[0023] 2. Saccharomyces cerevisiae

[0024] 2.1 Slant strains: culture medium: yeast extract 1wt%, peptone 2wt%, glucose 2wt%, agar 2wt%. Sterilize with 0.1Mpa for 20 minutes, inoculate aseptically, and incubate at 28°C for 48 hours.

[0025] 2.2 Conical flask shaker culture: culture medium: glucose 2wt%, urea 0.5wt%, ammonium sulfate 1wt%, yeast extract 0.5wt%. Sterilize with 0.1Mpa for 20min, inoculate aseptically, culture on a shaker at ...

Embodiment 2

[0064] The preparation method of the feed microecological preparation of this embodiment includes the following steps:

[0065] (1) Strain equipment: equipped with Lactobacillus acidophilus 32.1х10 8 cfu / L, Saccharomyces cerevisiae 1х10 8 cfu / L, Bacillus subtilis 61.5х10 8 cfu / L;

[0066] (2) Preparation of solid medium: In terms of weight percentage, mix the following raw materials uniformly: 80% bran, 15% apple pomace, 2% corn flour, 2% brown sugar, 0.5% dipotassium hydrogen phosphate and 0.5% ammonium sulfate; After sterilization, add water to adjust the water content to 12wt%, and pack it into a solid material bag (a plastic bag with a one-way air valve that can vent outward);

[0067] (3) Inoculation and fermentation: first mix Lactobacillus acidophilus and Saccharomyces cerevisiae strains in a volume ratio of 1:1, and then inoculate the above solid medium at an inoculum of 30wt% relative to the solid medium, at 30°C After 7 days of anaerobic fermentation, inoculate Bacillus su...

Embodiment 3

[0069] The preparation method of the feed microecological preparation of this embodiment includes the following steps:

[0070] (1) Strain equipment: equipped with Lactobacillus acidophilus 3х10 9 cfu / L, Saccharomyces cerevisiae not less than 1.1х10 8 cfu / L, Bacillus subtilis not less than 6х10 9 cfu / L;

[0071] (2) Preparation of solid medium: In terms of weight percentage, mix the following raw materials uniformly: 75% bran, 20% apple pomace, 1% corn flour, 3% brown sugar, 0.2% dipotassium hydrogen phosphate and 0.8% ammonium sulfate; After sterilization, add water to adjust the water content to 10wt%, and pack it into a solid material bag (a plastic bag with a one-way air valve that vents outward);

[0072] (3) Inoculation and fermentation: first mix Lactobacillus acidophilus and Saccharomyces cerevisiae strains in a volume ratio of 1:0.8, and then inoculate the solid medium at 25% by weight relative to the solid medium. After 8 days of anaerobic fermentation, inoculate Bacillus ...

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Abstract

The invention is a biological fermentation technology field, which involves a feed micro -ecological preparation and its preparation methods.The preparation method of the present invention includes the following steps: (1) Equipped with eorphoscin, winemark yeast, and Bacillus of Bacillus;Brown sugar, hydrogen phosphate, and ammonium sulfate; after sterilization, add water to adjust the water content; (3) vaccination and fermentation: use two vaccination and fermentation in stages.The microbial bacteria of the microorganisms of the microorganisms of the present invention grow well and have a large number, and the fermentation medium is acidic and has a strong taste, which is conducive to the use of feed.The preparation method of the present invention adopts secondary vaccination and fermentation in stages to provide good material conditions and growth environment.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a feed microecological preparation and a preparation method thereof. Background technique [0002] The research and application of animal microecological preparations can be traced back to 1947. Mollgaard first discovered that feeding piglets with Lactobacillus can effectively increase body weight and improve health. However, the middle and latter part of the last century was the golden age of antibiotic research, production and use, which made the research and development of probiotics at a low ebb. In recent years, due to the extensive use of antibiotics and synthetic drugs in the aquaculture industry, the safety hazards of animal-sourced food and environmental pollution have been caused, causing serious social, economic and ecological problems, and microecological preparations have become a research hotspot again. [0003] Feed probiotics are probi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/18A23K50/30A23K20/174A23K20/142A23K50/75A23K10/30A23K10/37A23K20/163A23K20/26
CPCY02P60/87
Inventor 杨建平赵志军刘永祥刘延贺胡华锋
Owner 凯帕雷森(江苏)生物科技有限公司
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