Method for determining content of tetramethylpyrazine in white liquor

A technology of tetramethylpyrazine and content, which is applied in the field of liquor analysis and detection, can solve the problem of tetramethylpyrazine being blank, etc., and achieves the effects of high requirements for instrument and equipment conditions, good feasibility and reliability, and simple and fast operation.

Inactive Publication Date: 2014-08-27
ANHUI XUANJIU GRP +1
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Problems solved by technology

However, the research on the detection of tetramethylpyrazi

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  • Method for determining content of tetramethylpyrazine in white liquor
  • Method for determining content of tetramethylpyrazine in white liquor
  • Method for determining content of tetramethylpyrazine in white liquor

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Example Embodiment

[0015] Example 1. Method for determining the content of tetramethylpyrazine in Xuanjiu products

[0016] 1. Prepare tetramethylpyrazine standard solution:

[0017] Tetramethylpyrazine was purchased from SIGMA, USA. Weigh 0.5 g of tetramethylpyrazine, dissolve it in chromatographic grade ethanol with a concentration of 60%, and make a constant volume to prepare a 500mg / L tetramethylpyrazine standard mother liquor.

[0018] Dilute the above-mentioned tetramethylpyrazine standard sample mother solution in multiples to prepare a 5mg / L tetramethylpyrazine standard sample solution (see figure 1 ).

[0019] Use a pipette to sequentially pipette the above-mentioned 1mL, 2mL, 4mL, 6mL, 8mL tetramethylpyrazine standard sample solution into a 10mL volumetric flask, dilute to the mark, that is to prepare 0.5mg / L, 1.0mg / L, 2.0mg / L, 3.0mg / L, 4.0mg / L tetramethylpyrazine standard solution, ready to use.

[0020] 2. Set the chromatographic detection conditions of the liquid chromatograph: use the Agi...

Example Embodiment

[0023] Example 2. The test of recovery, precision and detection limit of tetramethylpyrazine content in Xuanjiu wine samples by liquid chromatography.

[0024] Add 0.5mg / L, 1.0mg / L, 2.0mg / L tetramethylpyrazine standard solution to the liquor sample of the above-mentioned Example 1, respectively, and filter it with a membrane into the sample bottle of the liquid chromatograph. This experiment is done three times in parallel, and samples are injected sequentially in the order of low concentration to high concentration. After the wine sample is collected, the post-processing program is started, the method is loaded, and the result is output. Calculate the recovery rate and relative standard deviation of tetramethylpyrazine determined by this method (see Table 1).

[0025] Table 1 Determination of the recovery rate of tetramethylpyrazine in wine samples

[0026] (n=3, unit mg / L)

[0027]

[0028] Detection limit: The baseline noise value is 0.03mg / L, and the 3 times the baseline noise v...

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Abstract

The invention discloses a method for determining the content of tetramethylpyrazine in white liquor. The method comprises the following steps: setting relevant chromatographic detection conditions on a liquid chromatograph, introducing and determining prepared tetramethylpyrazine series standard solutions with different concentrations under the set chromatographic detection conditions, making a tetramethylpyrazine standard curve by treating the tetramethylpyrazine concentration of the introduced standard solutions as an ordinate and the determined peak area as an abscissa, introducing and determining a liquor sample to be determined under same chromatographic detection conditions, and outputting tetramethylpyrazine content data, that is, loading in the tetramethylpyrazine standard curve. A verification experiment result shows that the minimum quantitative limit of the method is 0.09mg/L, the linear correlation coefficient is greater than 0.999, the tetramethylpyrazine recovery rate is 94.67-100.33%, and the relative standard deviation is below 3.5%, so the method has the advantages of feasibility, reliability, high accuracy, simple and fast operation, low requirements on instrument conditions, and convenient popularization application.

Description

technical field [0001] The invention relates to a method for determining the content of tetramethylpyrazine in liquor, belonging to the technical field of liquor analysis and detection. technical background [0002] In recent years, with the progress of society and the development of economy, people have higher and higher requirements for the quality of life. Baijiu, as a traditional fermented product in China, has long been an indispensable part of the common people's table. Excavating the biologically active components (health function factors) in Chinese liquor, scientifically guiding consumers to drink healthily, and promoting the promotion of the liquor industry have been extensively studied by domestic and foreign scholars. Compared with distilled liquors from other countries, the content of aroma and taste substances in Chinese liquor is the highest in the world. Among them, pyrazine compounds, as important aroma and taste substances, have attracted more and more at...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 周萍张温清司冠儒唐莹史书俊王永军
Owner ANHUI XUANJIU GRP
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