Bacillus coagulans, Lactobacillus plantarum and Lactobacillus casei preparation and preparation thereof
A technology of Bacillus coagulans and Lactobacillus plantarum, applied in the field of feed microorganisms, can solve the problems of wastewater pollution, cumbersome operation of machinery and equipment, and difficulties in post-processing, etc., and achieve the effects of simple equipment structure, enhanced body immunity, and reduced breeding costs
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Embodiment 1
[0020] A kind of probiotics prepared by utilizing Bacillus coagulans and Lactobacillus plantarum, its preparation method comprises the steps:
[0021] Preparation of seed liquid: Bacillus coagulans HM-09, Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were inoculated into the medium containing liquid seeds from frozen glycerin tubes, and the Bacillus coagulans HM- 09 seed culture conditions: 37°C, 170 rpm shaker culture for 24 hours; Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were cultured in an incubator at 37°C for 24 hours;
[0022] The seed medium is: 2% peptone, 2% glucose, added water to 100%, pH 7.0-7.2.
[0023] solid state fermentation
[0024] Fermentation culture conditions: Add the Bacillus coagulans HM-09 seed solution to the solid medium for mixing and ferment for 12 hours, then inoculate the Lactobacillus plantarum HM-10 seed solution and Lactobacillus casei HM-09 seed solution and continue to ferment for 24 hours, total fermentati...
Embodiment 2
[0030] A kind of probiotics prepared by utilizing Bacillus coagulans and Lactobacillus plantarum, its preparation method comprises the steps:
[0031] Preparation of seed solution: Bacillus coagulans HM-09 was cultured in an incubator at 37°C for 24 hours on a shaker at 170 rpm; Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were cultured in an incubator at 37°C for 24 hours.
[0032] The seed medium is: peptone 2%, glucose 2%, add water to 100%, pH7.0-7.2;
[0033] Fermentation culture conditions: Add the Bacillus coagulans HM-09 seed solution to the solid medium, mix and ferment for 16 hours, then inoculate the seed solution of Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 and continue to ferment for 15 hours, the total fermentation time is 31 hours During the fermentation process, the fermentation temperature is controlled to be 37°C, the humidity is 80%, and the free amino nitrogen content is 486umol / g after the fermentation is completed;
[003...
Embodiment 3
[0039] A kind of probiotics prepared by utilizing Bacillus coagulans and Lactobacillus plantarum, its preparation method comprises the steps:
[0040] Preparation of seed solution: Bacillus coagulans HM-09 was cultured in an incubator at 37°C for 24 hours on a shaker at 170 rpm; Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 were cultured in an incubator at 37°C for 24 hours.
[0041] The seed culture medium of Bacillus coagulans HM-09, Lactobacillus plantarum HM-10 and Lactobacillus casei HM-09 is: peptone 2%, glucose 2%, add water to 100%, pH7.0-7.2;
[0042] Fermentation culture conditions: Add the Bacillus coagulans HM-09 seed liquid to the solid medium for mixing and ferment for 6 hours, then inoculate the seed liquid of Lactobacillus casei HM-09 and Lactobacillus plantarum HM-10 and continue to ferment for 36 hours, with a total fermentation time of 42 hours During the fermentation process, the fermentation temperature is controlled to be 37°C, the humidity ...
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