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A kind of extraction method of galantamine in lycoris

A technology of galantamine and extraction method, which is applied to the field of extraction of galantamine in Lycoris, can solve the problems of resource waste, environmental pollution, time-consuming extraction and the like, and achieves low cost, short extraction time, and extraction yield. high effect

Inactive Publication Date: 2017-02-22
邳州市滨河中小企业管理服务有限公司
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Problems solved by technology

Lycoris is one of the plant sources of galantamine raw material medicine, but its content is low, and its extraction usually adopts the solvent extraction method, which is time-consuming, wastes resources, and seriously pollutes the environment. Therefore, a new galantamine is provided. The extraction method of tamin is particularly important

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  • A kind of extraction method of galantamine in lycoris
  • A kind of extraction method of galantamine in lycoris
  • A kind of extraction method of galantamine in lycoris

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Embodiment Construction

[0020] Below in conjunction with specific accompanying drawing and test example the inventive method is described in further detail:

[0021] 1 Materials and methods

[0022] 1.1 Materials and reagents

[0023] Lycoris was collected from Guanzhuang, Yuanling County, Huaihua City, Hunan Province in March 2012.

[0024] Galantamine standard (purity > 98%) China National Institutes for Food and Drug Identification; Lycorine standard (purity > 98%) Shanghai Jinsui Biotechnology Co., Ltd.; Cellulase Huaian Baimai Green Bioenergy Co., Ltd. Pectinase such as methanol and acetonitrile from Ji Biotechnology (both chromatographically pure) from Tedia Corporation of the United States; concentrated sulfuric acid, potassium dihydrogen phosphate, and triethylamine are all analytically pure.

[0025] 1.2 Instruments and equipment

[0026] Agilent1260 High Performance Liquid Chromatograph from Agilent Corporation of the United States; Digital Display Water Bath Jintan Fuhua Instrument Co.,...

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Abstract

The invention provides an extraction method for galanthamine from lycoris radiate. The method includes following steps: (1) determination of chromatographic conditions: employing a Kromasil100-5C18 reversed-phase column (250mm*4.6mm, 5[mu]m) as an chromatographic column and an acetonitrile-phosphoric acid buffer solution (17:83) mixture as an mobile phase, and setting a column temperature to be 35 DEG C, a sample volume to be 20[mu]L and a detection wavelength to be 290nm, wherein the phosphoric acid buffer solution, which is 7.02 in pH value, is prepared by following steps: dissolving 2.72g of potassium dihydrogen phosphate in 100ml of water, adding 1.4ml of triethylamine and performing a diluting process to 1L; (2) establishment of a standard curve: establishing a regression equation according to the chromatographic conditions in the step (1); and (3) extraction of the galanthamine: weighing 2.0g of lycoris radiate. Herb powder, adding cellulase and pectinase, adding an aqueous solution with a pH value being 4.5 at a certain temperature for carrying out an extraction process for a certain time, performing a 5000r / min centrifuge process for 20 min, taking a supernatant, performing chromatographic measurement and calculating a yield of the galanthamine. The invention is low in extraction cost, is short in extraction time, is simple in technology, is free from polluting environment and is high in extraction yield.

Description

technical field [0001] The invention relates to a method for extracting galantamine, in particular to a method for extracting galantamine in Lycoris. Background technique [0002] Lycoris radiate.Herb is a perennial herb of the genus Lycoris of the Amaryllidaceae (Amaryllidaceae). It is mainly distributed in Asia. There are 17 species in my country. There are distributions. Amaryllis plants have high ornamental value and are known as "Chinese tulips". Amaryllis bulbs are rich in starch. If you grind them and wash them with plenty of water, eat them after detoxification, the taste is like glutinous rice flour. It is recorded in Materia Medica that it can be fried and soaked in water for overeating. Amaryllis is pungent, sweet, and warm in nature and has the functions of reducing swelling and killing insects. Its rich alkaloids are also widely used, especially galantamine. With the serious aggravation of population aging, Alzheimer's The burden of disease caused by silent di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/107
CPCC07D491/107
Inventor 田春莲王鹏刘小攀肖卓炳
Owner 邳州市滨河中小企业管理服务有限公司
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