Preparation method of phosphatidyl serine

A technology of phosphatidylserine and soybean lecithin, which is applied in the field of phospholipids, can solve the problems of difficult separation, complex by-products, and high production costs, and achieve the effects of simple overall process, easy industrialization, and low price

Active Publication Date: 2014-09-24
WENGYUAN GUANGYE QINGYI FOOD TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical synthesis method has many side reactions, complex reactions, complex by-products, and very difficult separation, such as the method disclosed in CN103351403 patent literature
Biological enzymatic method has great advantages and many studies, such as US5965413, US6492146, US7049107, US5700668, US6878532 and other patent documents, but the ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 100g crude soybean lecithin raw material (phosphatidylcholine content is 10%), add 50ml of water-saturated butyl acetate, stir and reflux at 120°C for 40 hours, evaporate the butyl acetate to dryness under reduced pressure, add 600ml methanol to reflux to dissolve the concentrate, methanol The layer was decompressed to recover methanol to obtain 55 g of methanol solubles. Add 275ml of dichloromethane to dissolve methanol soluble matter, and then configure 34.5ml of enzyme-containing aqueous solution, wherein the concentration of L-serine in the enzyme solution is 0.15g / ml, and the enzyme activity of phospholipase D in the enzyme solution is 4U / ml, adjust the pH is 6.3. The organic phase and the aqueous phase were mixed and reacted at 30° C. for 40 hours. The organic phase was separated after the reaction was completed, the organic phase was washed with 275ml of water, and the organic solvent was removed in vacuo to obtain 60 grams of concentrate, which was dissolved in...

Embodiment 2

[0022] 100g crude soybean lecithin raw material (phosphatidylcholine content is 11%), add 60ml water-saturated butyl acetate, stir and reflux at 127°C for 41 hours, evaporate the butyl acetate to dryness under reduced pressure, add 700ml methanol to reflux to dissolve the concentrate, methanol The layer was depressurized to recover methanol to obtain 57 g of methanol solubles. Add 456ml of dichloromethane to dissolve methanol-soluble matter, and then prepare 38ml of enzyme-containing aqueous solution, wherein the concentration of L-serine in the enzyme solution is 0.18g / ml, and the enzyme activity of phospholipase D in the enzyme solution is 4.5U / ml, adjust the pH is 6.4. The organic phase and the aqueous phase were mixed and reacted at 30° C. for 35 hours. The organic phase was separated after the reaction was completed, the organic phase was washed with 650ml of water, and the organic solvent was removed in vacuo to obtain 63 grams of concentrate, which was dissolved in 504...

Embodiment 3

[0024] 100g crude soybean lecithin raw material (phosphatidylcholine content is 17%), add 90ml of water-saturated butyl acetate, stir and reflux at 121°C for 48 hours, evaporate the butyl acetate to dryness under reduced pressure, add 800ml of methanol to reflux to dissolve the concentrate, methanol The layer was decompressed to recover methanol to obtain 62 g of methanol solubles. Add 372ml cyclohexane to dissolve methanol soluble matter, then configure 46.5ml of enzyme-containing aqueous solution, wherein the concentration of L-serine in the enzyme solution is 0.2g / ml, and the enzyme activity of phospholipase D in the enzyme solution is 4.7U / ml, Adjust the pH to 6.5. The organic phase and the aqueous phase were mixed and reacted at 35°C for 36 hours. The organic phase was separated after the reaction was completed, the organic phase was washed with 496ml of water, and the organic solvent was removed in vacuo to obtain 69 grams of concentrate, which was dissolved in 552ml of...

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Abstract

The invention discloses a preparation method of phosphatidyl serine, which comprises the following steps: taking coarse soybean lecithin as a raw material, adding water-saturated butyl acetate, stirring, and performing reflux degradation; evaporating the butyl acetate under reduced pressure, adding methanol into the concentrate, and performing reflux dissolution; recovering the methanol from the methanol layer under reduced pressure, thus obtaining a methanol solvend; preparing the methanol solvend and organic solvent into a solution, then preparing the organic phase and an enzyme-containing water solution into a water phase, mixing the organic phase and the water phase, and reacting; after the reaction is finished, separating out the organic phase, washing the organic phase with water, and removing the organic solvent in vacuum; and dissolving the concentrate with anhydrous ethanol, stirring, separating out an anhydrous ethanol insoluble substance, and performing vacuum drying to obtain the phosphatidyl serine product. Compared with the prior art, the preparation method has the following advantages: (1) the used raw material is the coarse soybean lecithin, so that the phosphatidylcholine content is low, and the price is low; and (2) the whole process is simple and easy to industrialize, the content of the prepared phosphatidyl serine is 40% or above, and the purity is high. Thus, the preparation method disclosed by the invention has important meanings for phospholipid industry, health food and medicine industry in China.

Description

technical field [0001] The invention belongs to the technical field of phospholipids, and in particular relates to a preparation method of phosphatidylserine. Background technique [0002] Phosphatidylserine (PS), also known as serine phospholipid, diacylglyceryl phosphoserine, referred to as PS, is a kind of ubiquitous phospholipid, usually located in the inner layer of cell membrane, phosphoglycerides in phospholipid compounds, is the cell membrane One of the components related to a series of membrane functions. Especially in the nervous system of the human body, it is one of the important components of the cell membrane of the brain. At the same time, it plays an important role in regulating various functions of the brain (especially the memory and emotional stability of the brain). fluidity, permeability, and can activate the metabolism and synthesis of various enzymes. The existing synthetic methods include chemical synthesis and biological enzymatic synthesis, and th...

Claims

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Application Information

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IPC IPC(8): C12P13/06
Inventor 杜阳吉梁丽敏刘春凤
Owner WENGYUAN GUANGYE QINGYI FOOD TECH
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