A kind of preparation method of phosphatidic acid

A technology of phosphatidic acid and phospholipase, applied in the field of phospholipids, can solve the problems of cumbersome process, high cost, and less preparation, and achieve the effects of simple process, low energy consumption, low cost, and low price

Active Publication Date: 2019-04-16
WENGYUAN GUANGYE QINGYI FOOD TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned existing methods generally have the disadvantages of less preparation amount, complicated process and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 100g of raw soybean lecithin (with a phosphatidylcholine content of 10%), add it to 1000mL of butyl acetate and stir to dissolve, then configure 1000mL of an enzyme-containing aqueous solution, in which the enzyme activity of phospholipase D in the enzyme solution is 4U / mL, adjust The pH is 5.6. The organic phase and the aqueous phase were mixed and reacted at 37°C for 30 hours. After the reaction was completed, the organic phase was separated, and the organic solvent was removed under reduced pressure at 50° C. to obtain 85 g of a concentrate. The concentrate was dissolved in 500 mL of 90% methanol at 40°C and stirred for 3 hours, then cooled to -10°C, kept for 8 hours, and the insoluble matter was separated, and the insoluble matter was dried under reduced pressure to obtain 60 grams of methanol insoluble matter. Methanol insolubles were separated and purified through 600 grams of 200 mesh silica gel column, using n-hexane as the eluent, TLC tracking and monitor...

Embodiment 2

[0023] 200g crude sunflower seed lecithin raw material (phosphatidylcholine content is 15%), add 1000mL butyl acetate and stir to dissolve, and then prepare 1000mL enzyme-containing aqueous solution, in which the activity of phospholipase D in the enzyme solution is 5U / mL, adjust The pH is 6.5. The organic phase and the aqueous phase were mixed and reacted at 30° C. for 40 hours. After the reaction was completed, the organic phase was separated, and the organic solvent was removed under reduced pressure at 50° C. to obtain 178 g of a concentrate. The concentrate was dissolved with 2000mL of 95% methanol at 48°C and stirred for 4 hours, then cooled to -10°C, kept warm for 16 hours, and the insoluble matter was separated, and the insoluble matter was dried under reduced pressure to obtain 128 grams of methanol insoluble matter. Methanol insolubles were separated and purified through 2600 grams of 300-mesh silica gel column, using n-hexane as the eluent, TLC tracking and monitor...

Embodiment 3

[0025] Add 100g of raw soybean lecithin (16% phosphatidylcholine content) into 1000mL of ethyl acetate and stir to dissolve, then configure 500mL of enzyme-containing aqueous solution, in which the enzyme activity of phospholipase D in the enzyme solution is 4U / mL, adjust the pH is 5.6. The organic phase and the aqueous phase were mixed and reacted at 37°C for 30 hours. After the reaction was completed, the organic phase was separated, and the organic solvent was removed under reduced pressure at 50° C. to obtain 87 g of a concentrate. The concentrate was dissolved in 700mL of 95% methanol at 45°C and stirred for 3.5 hours, then cooled to 0°C, kept warm for 12 hours, and the insoluble matter was separated, and the insoluble matter was dried under reduced pressure to obtain 70 grams of methanol insoluble matter. Methanol insolubles were separated and purified through 1050 g of 200 mesh silica gel column, using n-hexane as the eluent, TLC tracking and monitoring until the eluen...

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Abstract

The invention discloses a preparation method of phosphatidic acid. The preparation method includes the following steps that rough lecithin is taken and dissolved in an organic solvent to be prepared intro a rough lecithin solution, phospholipase D is taken and prepared into a phospholipase D aqueous solution, the rough lecithin solution and the phospholipase D aqueous solution are mixed to perform an enzyme catalysis reaction, an organic phase is separated out after the reaction ends, the organic phase is dried in a concentration mode, concentrated matter is obtained and extracted through an extracting agent and precipitates at low temperature, undissolved matter in the extracting agent is taken after heat preservation, the undissolved matter in the extracting agent passes a chromatography column after being dried in a concentration mode, obtained products are dried to remove eluting liquid after an eluting solvent is used for elution, and then high-purity phosphatidic acid is obtained. The soybean or sunflower seed rough lecithin serves as raw materials, and therefore the price is low as the phosphatidylcholine content is low. According to the method, the overall technology is simple, energy consumption and cost are low, large-scale industrialization can be achieved easily, the content of prepared phosphatidylcholine is 60% or above, purity is high, and the method has great significance in the phospholipids industry, health-care food and the pharmaceutical industry in China.

Description

technical field [0001] The invention belongs to the technical field of phospholipids, and in particular relates to a preparation method of phosphatidic acid. Background technique [0002] Phosphatidic acid (PA) is a simple phospholipid, the basic component of animal and plant cell membranes, nuclear membranes, and plastid membranes, and the basic substance of life. Fatty acid is a kind of acidic phospholipid, which is composed of glycerol group, fatty acid group and phosphoric acid group. Fatty acid groups are often palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid and the like. From the chemical structure point of view, PA is an amphoteric compound, one end of the fatty acid group has strong lipophilicity, and one end of the phosphoric acid group has strong hydrophilicity. Phosphatidic acid is an intermediate in the synthesis of glycerides and most phospholipids, although its content in organisms is very small, and phosphatidic acid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P9/00
CPCC12P7/6481
Inventor 杜阳吉梁丽敏刘春凤卫娜
Owner WENGYUAN GUANGYE QINGYI FOOD TECH
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