Three-dimensional nanometer chip, method for fractionation detection on circulating tumor cells (CTC) by using chip, and application of three-dimensional nanometer chip

Abstract

The invention relates to a three-dimensional nanometer chip and a method for fractionation detection on circulating tumor cells (CTC) by suing the chip, and application of the three-dimensional nanometer chip. The method comprises the steps: stripping and slicing a silicon wafer, heating in a piranha solution, then washing with water, drying by blowing, soaking in a 2 percent APTES ((3-aminopropyl)trimethoxysilane) solution for 2h, respectively cleaning the silicon wafer with absolute ethyl alcohol and deionized water, immersing in a 2.5 percent glutaraldehyde solution for 1h, putting in a culture chamber slide, uniformly dropping 10microgram/ml anti-EpCAM (epoxy resin cell adhesion molecules) on the silicon wafer, reacting for 0.5h at a room temperature, washing out redundant antibodies with a PBS (Phosphate Buffer Solution), and closing by using 25 microL of 1 percent BSA (Bovine Serum Albumin) solution for 1h to prepare the chip, wherein the chip can be used for capturing the circulating tumor cells in peripheral blood. The novel and convenient CTC capturing chip disclosed by the invention is capable of efficiently capturing the tumor cells in a laboortary; and compared with the prior art, the method has the advantages of being simpler, lower in cost and huge in clinical value.

Description

[technical field] [0001] The invention relates to the technical field of tumor cell detection equipment and methods, in particular to a three-dimensional silicon nanochip and a method for separating and detecting circulating tumor cells and its application. [Background technique] [0002] Malignant tumor cells acquire the ability to invade and cause distant metastasis is the main cause of their death. Blood supply metastasis is one of the important ways of metastasis. Tumor cells shed from the primary lesion into the blood circulation and invade other organs for distant metastasis. Therefore, the detection of circulating tumor cells (CTCs) in peripheral blood has important clinical significance. As a "liquid biopsy specimen" representing the primary tumor, CTCs have similar or even the same biological characteristics as the primary lesion. But for the tens of thousands of peripheral blood cells, the content of CTCs in peripheral blood is negligible, about every 1×10 5 ~1×1...

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to solve the technical defects of low capture CTC efficiency and high capture cost in the prior art, and use black silicon as the substrate through DRIE (that is, Deep Reactive Ion Etching) technology High-efficiency capture CTC chip

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