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Method for synthesizing artificial antigen of progesterone

An artificial antigen and synthesis method technology, applied in chemical instruments and methods, hybrid peptides, animal/human proteins, etc., can solve the problems of cumbersome processing process, inability to meet rapid detection, and time-consuming detection, and achieve a simple synthesis process. Effect

Active Publication Date: 2014-10-08
深圳重链生物科技有限公司
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Problems solved by technology

These methods have accurate detection results and high sensitivity, but the sample pretreatment process is cumbersome and time-consuming, which cannot meet the needs of rapid detection in large quantities on site

Method used

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  • Method for synthesizing artificial antigen of progesterone
  • Method for synthesizing artificial antigen of progesterone
  • Method for synthesizing artificial antigen of progesterone

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Embodiment 1

[0027] (1) Preparation of progesterone hapten: 34.4 mg (0.1 mmol) of 17α-methyl-17β-hydroxyandrost-4-en-3-one was dissolved in 3 mL of anhydrous dimethyl sulfoxide, and potassium hydroxide powder was added 208.5mg (1.5mmol), the solution is light yellow, called A liquid. 27.8 mg (0.2 mmol) of 2-bromoacetic acid was dissolved in 1.4 mL of anhydrous dimethyl sulfoxide, slowly added dropwise to solution A, the color gradually changed to dark yellow, and reacted at room temperature for 4 hours. Terminate the reaction with 50 mL of ice water, extract three times with ethyl acetate, combine the water phases, acidify the water phase with 2 mol / L hydrochloric acid to obtain a yellow precipitate, wash with water four times until neutral, and obtain the yellow progesterone hapten (P- hapten) powder. The product was identified by LC-MS after dissolving in methanol. Identification results such as figure 1 As shown, the target mass-to-core ratio of the mass spectrum is 402, negative ion...

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Abstract

The invention discloses a method for synthesizing an artificial antigen of progesterone, belonging to the technical field of biochemical industry. Specific hydroxyl on a progesterone analogue 17alpha-methyl-17beta-hydroxy steroid-4-alkene-3-ketone is utilized as a reaction group, and reacts with a 2-bromoacetic acid to obtain a progesterone hapten; and the hapten is connected with carrier protein bull serum albumin (BSA) to synthesize the artificial antigen of the progesterone by adopting a mixed anhydride method or the hapten is connected with carrier protein OVA to synthesize a coating antigen. The artificial antigen of the progesterone is successfully synthesized by the method; the synthesis process is simple; a plurality of parts with the same steroid hormone ring structures are fully exposed by the synthesis product; a group-selectivity progesterone monoclonal antibody can be obtained when the method is applied to immunoassay; and the national requirements of multiplex detection on progestational hormone are met.

Description

technical field [0001] The invention relates to a method for synthesizing a progesterone artificial antigen. The obtained novel artificial antigen can be used in the field of immune analysis and belongs to the technical field of biochemical engineering. Background technique [0002] Progesterone (P) is a natural progesterone, which is secreted by the corpus luteum of the ovary and is necessary to maintain pregnancy. It is also called progesterone and is a gestational hormone. In clinical medicine, it is mainly used for habitual abortion, dysmenorrhea, menorrhagia or blood collapse, amenorrhea, etc. A large amount of oral administration is also used for premenstrual syndrome, menstrual disorders caused by cessation of ovulation, benign breast disease, premenopause and menopause, etc. Progesterone is an endogenous hormone that can be produced by the body. A large amount of intake in the first trimester can lead to an increased probability of deformities in the fetal spine, an...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K14/77
CPCC07K14/765C07K14/77C07K19/00
Inventor 胥传来孔娜匡华徐丽广马伟刘丽强宋珊珊吴晓玲
Owner 深圳重链生物科技有限公司
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