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Method for measuring methyleugenol in cigarette mainstream smoke

A technology of cigarette mainstream smoke and methyl eugenol, which is applied in the field of cigarette physical and chemical index detection, can solve the problems of inability to achieve selective elution, inability to accurately quantitatively analyze target substances, and poor retention of solid-phase extraction columns. Achieve the effect of reducing error, improving sensitivity and ensuring accuracy

Active Publication Date: 2014-10-08
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, based on repeated verification of the method, it was found that the retention of methyl eugenol on the CN-bonded solid-phase extraction column used in the literature was not strong, the purpose of selective elution could not be achieved, and the target could not be accurately quantitatively analyzed at all. thing

Method used

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  • Method for measuring methyleugenol in cigarette mainstream smoke
  • Method for measuring methyleugenol in cigarette mainstream smoke
  • Method for measuring methyleugenol in cigarette mainstream smoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for measuring methyl eugenol in cigarette mainstream smoke, flow chart as figure 1 shown, including the following steps:

[0036] (1) Preparation of internal standard solution

[0037] Internal standard stock solution: Accurately weigh 0.1069g of internal standard 3,4-methylenedioxyacetophenone, and dilute to 100mL with ethanol to obtain an internal standard stock solution with a concentration of 1069μg / mL; internal standard solution: accurately pipette Take 1mL of internal standard stock solution, and dilute to 100mL with ethanol to obtain an internal standard stock solution with a concentration of 10.69μg / mL.

[0038] (2) Preparation of standard working solution

[0039] Primary standard stock solution: Accurately weigh 0.1050mg methyl eugenol, dilute to 100mL with ethanol to obtain a primary standard stock solution with a concentration of 1050μg / mL; secondary standard stock solution: accurately pipette 0.5mL primary standard Stock solution, dilute to 100m...

Embodiment 2

[0062] Taking a cigarette sample as the analysis object, adopt the method of embodiment 1 to measure the content of target substance, carry out precision experiment, measure altogether 6 times, calculate the relative standard deviation (RSD) of 6 parallel determination results respectively, measurement result sees the following table 3. The results in the table show that the RSD of the repeatability of the experimental method is less than 5%. For the analytical detection of trace substances, the method has good precision.

[0063] Table 3 Precision of the method

[0064] serial number

Determination result (ng / branch)

1

2.89

2

2.69

3

2.75

4

2.76

5

2.56

6

2.63

RSD(%)

4.30

[0065] Taking a cigarette sample as an object, carry out standard addition treatment at three different concentration levels of low, medium and high, and then perform treatment according to the above standard pr...

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Abstract

The invention discloses a method for measuring methyleugenol in cigarette mainstream smoke. The method comprises the following steps: preparing an internal standard solution, a standard working solution and a sample solution, performing gas chromatography-tandem mass spectrometry analysis and calculating the measurement result. 3,4-methylene dioxo acetophenone serves as an internal standard substance for quantifying, the optimized detection method is simple and convenient in operation, sensitive in response and accurate in quantitative analysis, interference brought by a complex sample matrix is effectively reduced, the target has good signal response and has high linear correlation due to the adopted chromatography-mass spectrum conditions, the detection limit is 0.31ng per cigarette, the relative standard deviation is 4.30 percent, and the adding standard recovery is between 94.85 percent and 112.51 percent. Therefore, the method is high in sensitivity, high in repeatability and high in recovery rate and is suitable for quantitative analysis of trace targets in the complex smoke matrix.

Description

technical field [0001] The invention belongs to the technical field of cigarette physical and chemical index detection, and in particular relates to a method for measuring methyl eugenol in cigarette mainstream smoke. Background technique [0002] Methyl eugenol, also known as eugenol methyl ether, L-rose ether, eugenol methyl ether, O-methyl eugenol, 4-allyl veratrol, 4-allyl veratrol, eugenyl methyl Methyl Eugenol (Eugenyl Methyl Ether). The CAS number is 93-15-2 and the molecular formula is C 11 h 14 o 2 , molecular weight 178.23, colorless to slightly yellow liquid. Boiling point 249°C, relative density 1.032-1.036, refractive index 1.532-1.536, flash point 99°C, soluble in ethanol and oil. There is a sweet clove-fennel spicy aroma, like carnation, the aroma is more transparent and long-lasting, and it has a mild spicy aroma like tea. According to the classification of toxicity grade, methyl eugenol is poisoned. According to MSDS data, its LD50 (rat, oral) is 1179...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 廖惠云庄亚东石怀彬曹毅刘献军李朝建王珂清张媛韩开冬朱龙杰朱莹刘琪
Owner CHINA TOBACCO JIANGSU INDAL
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