Unlock instant, AI-driven research and patent intelligence for your innovation.
Preparation method and use method of apramycin-detection colloidal gold test paper strip
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of colloidal gold test paper and apramycin, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost of detection, weakness of animal body, unfavorable promotion and application, etc.
Inactive Publication Date: 2014-10-08
JIANGSU WISE SCI & TECH DEV
View PDF4 Cites 3 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Apramycin is contained in many veterinary medicines, but excessive use may cause weakness of the animal body, so the dosage should be appropriate
In food and feed, the detection methods of apramycin include thin-layer chromatography, gas chromatography, gas-masscoupling and high-performance liquid chromatography. Since the sample pretreatment of the above analysis methods is relatively complicated, special personnel are required for operation. Expensive technical personnel and testing costs are not conducive to popularization and application
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0029] 安普霉素检测卡是在检测卡外壳1中设置检测试纸条2,检测卡外壳1的结构及检测试纸条2的设置见附 figure 1, the detection card shell 1 is a long flat thin shell shell with a length of 70 mm, a width of 20 mm, a thickness of 5 mm, and a thin shell wall thickness of 1 mm. It is made of engineering plastics. The detection card shell 1 consists of an upper cover and a lower cover. It is formed by connecting, and there are detection window holes 3 and sample injection holes 4 on the upper cover of the detection card casing 1. The detection test strip 2 is placed in the lower cover of the detection card casing 1 .
[0030] The detection test strip 2 is a narrow sheet with a multi-layer structure, the length of the sheet is 60 mm, the width of the sheet is about 4 mm, and the thickness is less than 2.5 mm. The detection test strip 2 has a multi-layer structure: the bottom layer is a supporting backboard 5, which is a polyethylene sheet with a thickness of about 0.5 mm, a length of 60 mm, and a width of 4 mm; The thickness of...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
length
aaaaa
aaaaa
thickness
aaaaa
aaaaa
Login to View More
Abstract
The invention relates to a preparation method and a use method of a colloidal gold test paper strip for fast detection of apramycindrug residues. The preparation method comprises orderly pasting a sample pad, a gold marking pad, a cellulosenitrate membrane and a sample absorption pad on the backing of a test paper. The surface of the cellulosenitrate membrane is coated with a detection antigen strip as a detection line and with an anti-second specie animal protein IgG strip as a reference line. The apramycin-detection colloidal gold test paper strip for fast detection has strong singularity, can realize semi-quantitative detection, can be used at an environment temperature of 4-40 DEG C, is suitable for fast detection of apramycin residues of animal-derived foods by individual farmers, food hygienequality control department and customhouse. The apramycin-detection colloidal gold test paper strip has strong singularity and high sensitivity, can realize semi-quantitative detection and can be operated simply and conveniently.
Description
technical field [0001] 本发明属于食品安全检测领域,具体涉及食品中安普霉素药物残留的一种快速胶体金试纸条的制备及使用方法。 Background technique [0002] 安普霉素又名阿普拉霉素,易溶于水,稍溶于低级醇,不溶于其他有机溶剂。 紫外吸收光谱为末端吸收为红外光谱具有典型的氨基糖苷类抗生素的多氨基、多羟基特征吸收峰。 是一种黑暗链菌产生的氨基糖苷类新兽用抗生素,20世纪80年代由美国开发成功,其特点是抗菌谱广,不易产生抗药性,可用于鸡大肠杆菌、沙门氏杆菌和支原体引起的 Infect. 作为药物型饲料添加剂,安普霉素能明显促进增重和提高饲料转化率,在畜禽养殖领域得到广泛应用。 主要用于家禽的大肠杆菌、沙门氏杆菌感染,疗效非常显著,被美国FDA推荐为治疗大肠杆菌病的首选药物,对支原体病也有效。 [0003] 安普霉素平时也称其为硫酸安普霉素,是一种抗菌性抗生素。很多兽药当中都包含安普霉素,但使用过多可能导致动物机体虚软,所以用量要适当。在食品和饲料中,安普霉素的检测方法有薄层色谱法,气象色谱法,气-质联机和高效液相色谱法,由于以上诸分析法样品前处理比较复杂,操作时需专门的技术人员、检测费用昂贵,不利于推广应用。 Contents of the invention [0004] 本发明的目的是为了克服现有技术的不足,提供一种特异性强,灵敏度高,且操作简单方便,对样品经过简单处理即可检测的安普霉素快速试纸半定量检测方法。 [0005] 本发明的目的可通过以下技术方案来实现: [0006] 安普霉素胶体金试纸条检测方法其技术要点是:试纸的背衬上依次贴有样品垫和金标垫和硝酸纤维素膜和吸水吸水纸,金标垫上被标记的物质为第二种属动物蛋白与安普霉素检测用抗原的混合物或第二种属动物蛋白与安普霉素抗体的混合物;硝酸纤维素膜上包被有安普霉素抗体1条作为检测线,同时还包被有抗第二种属动物蛋白的IgG 1条作为参考线或包被有安普霉素检测用抗原1条作为检测线,同时还包被有抗第二种属动物蛋白的工IgG1条作为参考线。在试纸制备时通过调节检测线和参考线包被物的浓度,控制检测时检测线和参考线的显色深浅,并将其显色深浅与标准物质浓度对应起来,达到半定量检测。 [0007] 所述的检测用抗原是指安普霉素与载体物...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
