Preparation method and use method of apramycin-detection colloidal gold test paper strip

Abstract

The invention relates to a preparation method and a use method of a colloidal gold test paper strip for fast detection of apramycin drug residues. The preparation method comprises orderly pasting a sample pad, a gold marking pad, a cellulose nitrate membrane and a sample absorption pad on the backing of a test paper. The surface of the cellulose nitrate membrane is coated with a detection antigen strip as a detection line and with an anti-second specie animal protein IgG strip as a reference line. The apramycin-detection colloidal gold test paper strip for fast detection has strong singularity, can realize semi-quantitative detection, can be used at an environment temperature of 4-40 DEG C, is suitable for fast detection of apramycin residues of animal-derived foods by individual farmers, food hygiene quality control department and customhouse. The apramycin-detection colloidal gold test paper strip has strong singularity and high sensitivity, can realize semi-quantitative detection and can be operated simply and conveniently.

Description

technical field [0001] 本发明属于食品安全检测领域,具体涉及食品中安普霉素药物残留的一种快速胶体金试纸条的制备及使用方法。 Background technique [0002] 安普霉素又名阿普拉霉素,易溶于水,稍溶于低级醇,不溶于其他有机溶剂。 紫外吸收光谱为末端吸收为红外光谱具有典型的氨基糖苷类抗生素的多氨基、多羟基特征吸收峰。 是一种黑暗链菌产生的氨基糖苷类新兽用抗生素,20世纪80年代由美国开发成功,其特点是抗菌谱广,不易产生抗药性,可用于鸡大肠杆菌、沙门氏杆菌和支原体引起的 Infect. 作为药物型饲料添加剂,安普霉素能明显促进增重和提高饲料转化率,在畜禽养殖领域得到广泛应用。 主要用于家禽的大肠杆菌、沙门氏杆菌感染,疗效非常显著,被美国FDA推荐为治疗大肠杆菌病的首选药物,对支原体病也有效。 [0003] 安普霉素平时也称其为硫酸安普霉素,是一种抗菌性抗生素。很多兽药当中都包含安普霉素,但使用过多可能导致动物机体虚软,所以用量要适当。在食品和饲料中,安普霉素的检测方法有薄层色谱法,气象色谱法,气-质联机和高效液相色谱法,由于以上诸分析法样品前处理比较复杂,操作时需专门的技术人员、检测费用昂贵,不利于推广应用。 Contents of the invention [0004] 本发明的目的是为了克服现有技术的不足,提供一种特异性强,灵敏度高,且操作简单方便,对样品经过简单处理即可检测的安普霉素快速试纸半定量检测方法。 [0005] 本发明的目的可通过以下技术方案来实现: [0006] 安普霉素胶体金试纸条检测方法其技术要点是:试纸的背衬上依次贴有样品垫和金标垫和硝酸纤维素膜和吸水吸水纸,金标垫上被标记的物质为第二种属动物蛋白与安普霉素检测用抗原的混合物或第二种属动物蛋白与安普霉素抗体的混合物;硝酸纤维素膜上包被有安普霉素抗体1条作为检测线,同时还包被有抗第二种属动物蛋白的IgG 1条作为参考线或包被有安普霉素检测用抗原1条作为检测线,同时还包被有抗第二种属动物蛋白的工IgG1条作为参考线。在试纸制备时通过调节检测线和参考线包被物的浓度,控制检测时检测线和参考线的显色深浅,并将其显色深浅与标准物质浓度对应起来,达到半定量检测。 [0007] 所述的检测用抗原是指安普霉素与载体物...

AI Technical Summary

Problems solved by technology

Apramycin is contained in many veterinary medicines, but excessive use may cause weakness of the animal body, so the dosage should be appropriate

In food and feed, the detection methods of apramycin include thin-layer chromatography, gas chromatography, gas-mass coupling and high-performance liquid chromatography. Since the sample pretreatment of the above analysis methods is relatively complicated, special personnel are required for operation. Expensive technical personnel and testing costs are not conducive to popularization and application

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