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A method for efficiently extracting and producing Wulingshen mycelium polysaccharides by submerged liquid fermentation

A technology of mycelium polysaccharides and extraction methods, which is applied in the field of microorganisms, can solve the problems of high product cost, increased pollution control cost, and long operation cycle, and achieve the goal of reducing the content of impurity proteins, reducing pollution control costs, and reducing labor intensity Effect

Active Publication Date: 2016-04-27
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this process is that a large amount of water needs to be added for a long time to extract, and in order to increase the yield, it needs to be extracted repeatedly 2-3 times. %, product cost is more expensive
And the separated supernatant is directly discarded as waste liquid, which increases the cost of pollution control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for efficiently extracting deep liquid fermentation to produce polysaccharide of Wulingshen mycelium according to the present invention is carried out according to the following steps:

[0030] 1. Preparation of Wulingshen fermentation broth: The strain of Wulingshen was purchased from the Institute of Microbiology, Chinese Academy of Sciences (strain code CGMCCNO.1038). The activated Uling ginseng shake flask strain was inoculated into the seed medium (10L) according to the inoculum amount of 10% (v / v), wherein the seed medium was composed of 2% glucose, 0.2% peptone, 1% soybean meal powder, and phosphoric acid. Potassium dihydrogen 0.3%, magnesium sulfate heptahydrate 0.15%, zinc sulfate 0.10%, soybean oil 0.02%, the pH value was adjusted to 5.5, the ventilation ratio was 1:0.3vvm, the tank pressure was 0.03Mpa, and the culture was shaken at 26-28°C for 4 days. Wuling ginseng seed liquid; the seed liquid is inserted into a fermentation medium (50L) accordin...

Embodiment 2

[0038] The present invention efficiently extracts the method for producing Wulingshen mycelium polysaccharide by deep layer liquid fermentation according to the following steps:

[0039] 1. Preparation of Wulingshen fermentation broth: Wulingshen strains were purchased from the Institute of Microbiology, Chinese Academy of Sciences (strain code CGMCCNO.1038). The activated Wulingshen shake flask strain was inoculated in the seed medium (20L) according to the inoculum size 12% (v / v), wherein the seed medium consisted of 2% glucose, 0.2% peptone, 1% soybean cake powder, phosphoric acid Potassium dihydrogen 0.3%, magnesium sulfate heptahydrate 0.15%, zinc sulfate 0.10%, soybean oil 0.02%, adjust the pH value to 5.5, the ventilation ratio is 1:0.3vvm, the tank pressure is 0.03Mpa, shake culture at 26~28℃ for 4 days. Wuling ginseng seed liquid; the seed liquid is inserted into the fermentation medium (100L) according to the inoculum size 15% (v / v), wherein the fermentation medium c...

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Abstract

The invention discloses a method for efficiently extracting xylariasp mycelium polysaccharide produced through submerged fermentation. According to the method, mycelium separated by xylariasp fermentation liquid is treated by ultrasonic waves and composite enzymatic hydrolysis and is separated to obtain the xylariasp polysaccharide. By means of the method, a conventional water extraction and alcohol precipitation method for extracting polysaccharide is changed, the extraction yield of the xylariasp polysaccharide and the product quality are improved, the use amount of purified water and energy consumption during production are reduced, and the economic benefits are increased.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a method for efficiently extracting deep liquid fermentation to produce polysaccharide of Wulingshen mycelium. Background technique [0002] Wulingshen belongs to Ascomycetes, Charcoal Ceratocystis, and is a traditional medicinal fungus. It grows on the abandoned ant nests of black-winged soil-dwelling termites deep underground. It is regarded as a rare and precious medicinal material by the research and application fields of medicinal fungi in the world, and has a wide range of medicinal value. [0003] Wild black ginseng is mainly produced in Sichuan, Yunnan, Guangdong and other places, and also grows in Zhejiang, Jiangxi, and Jiangsu. Because Wulingshen grows on the abandoned ant nests of soil-dwelling termites 2 meters to 20 meters underground, it is very difficult to extract, so the natural resources are extremely scarce. Wulingshen is both a medicinal fungus and an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 王文风龚劲松徐国华徐玲王英燕杨亚威高岩张芙蓉
Owner JIANGSU SHENHUA PHARMA
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