A method for acclimating black boletus with excellent traits

An excellent character, the technology of black boletus, applied in the directions of botanical equipment and methods, fertilizer mixture, horticulture, etc., can solve the constraints of the expansion of black boletus cultivation, large-scale cultivation and industrialization, decomposition and utilization of lignin and cellulose. Weakness and poor saprophytic ability of black boletus, to achieve the effect of improving biological efficiency, improving anti-impurity ability and excellent characters

Active Publication Date: 2016-03-30
贵州宏臻菌业投资发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the nutritional method of black boletus (Phlebopus portentosu) is unique, which is different from other genera of Boletaceae, that is, it can live a saprophytic life away from symbiotic tree species, but the saprophytic ability of black boletus is not strong, mainly because of the black Boletus cannot decompose and utilize lignin, cellulose or its ability to decompose and utilize lignin and cellulose is weak. Therefore, the bacterial strains that can be collected from wild fruiting bodies only account for 3%-5%, while Among the strains that produce mushrooms, they have a certain ability to resist miscellaneous bacteria, so that they will not be polluted after being covered with soil. The fruiting rate of mushroom bags and the maturity rate of fruiting mushroom bags reach 75%. The average single mushroom weight of mature fruit bodies is only 80g 1%-3%, therefore, it is particularly difficult to screen a bacterial strain possessing commercial cultivation traits from wild boletus strains, which restricts the expanded cultivation, scale cultivation and industrialization of black boletus

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  • A method for acclimating black boletus with excellent traits
  • A method for acclimating black boletus with excellent traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Collection of wild boletus fruiting bodies

[0043] Collect 50 fruiting bodies of wild black boletus, the fruiting body requires a single mushroom weight of 400-500g, the stipe is thick and not hollow, the flesh is thick, the medium mature pore is not opened, no insects and miscellaneous bacteria, fresh;

[0044] Use the tissue separation method to cut out the meat tissue at the junction of the fruiting body cap and the stipe and inoculate it in the medium of the mother species, cultivate it at a constant temperature of 30°C, and obtain the hyphae of the mother species after 25 days;

[0045] Wherein, the mother seed culture medium includes the following components, by weight: 200 parts of potatoes, 20 parts of glucose, 2 parts of yeast extract juice, 2 parts of ammonium nitrate, MgSO 4 2 copies, KH 2 PO 4 2 parts, ZnSO 4 1 part, MnSO 4 0.05 parts, 22 parts of agar powder, 1100 parts of water, and adjust the pH to 6.5.

[0046] 2. the wild black boletus that col...

Embodiment 2

[0067] 1. Collection of wild boletus fruiting bodies

[0068] Collect 100 fruiting bodies of wild black boletus, the fruiting body requires a single mushroom weight of 200-250g, the stipe is thick and not hollow, the flesh is thick, the medium mature pore is not opened, no insects and bacteria, fresh;

[0069] Use the tissue separation method to cut the meat tissue at the junction of the fruiting body cap and the stipe and inoculate it in the mother seed medium, culture at a constant temperature of 28°C, and obtain the mother seed hyphae in 30 days;

[0070] Wherein, the mother seed medium includes the following components, by weight: 100 parts of potatoes, 10 parts of glucose, 1 part of yeast extract, 1 part of ammonium nitrate, MgSO 4 1 part, KH 2 PO 4 1 part, ZnSO 4 0.5 parts, MnSO 4 0.01 parts, 18 parts of agar powder, 900 parts of water, and adjust the pH to 4.5.

[0071] 2. the wild black boletus that collects is multiplied

[0072] Inoculate the female hyphae obta...

Embodiment 3

[0092] 1. Collection of wild boletus fruiting bodies

[0093] Collect 80 wild boletus fruiting bodies, the fruiting body requires a single mushroom weight of 300-350g, the stipe is thick and not hollow, the flesh is thick, the medium mature pore is not opened, no insects and bacteria, fresh;

[0094] Use the tissue separation method to cut out the meat tissue at the junction of the fruiting body cap and the stipe and inoculate it in the medium of the mother species, cultivate it at a constant temperature of 29°C, and obtain the hyphae of the mother species after 28 days;

[0095] Wherein, the mother seed medium includes the following components, by weight: 150 parts of potatoes, 15 parts of glucose, 2 parts of yeast extract, 1 part of ammonium nitrate, MgSO 4 2 servings, KH 2 PO 4 1 part, ZnSO 4 0.8 parts, MnSO 4 0.03 parts, 20 parts of agar powder, 1000 parts of water, and adjust the pH to 6.

[0096] 2. the wild black boletus that collects is multiplied

[0097] Inocul...

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Abstract

The invention relates to the field of edible mushroom strain domestication, in particular to a phlebopus portentosu good character domestication method. The phlebopus portentosu good character domestication method comprises the following steps that (a) phlebopus portentosu sporocarp with good characters is selected as domestication strains; (b) context tissue is inoculated into a nutrition barren type matrix, arranged at 26-30 DEG C and cultivated in dark light until mycelia are fully grown; (c) front mycelia are extracted and inoculated into the nutrition barren type matrix until mycelia are fully grown; (d) the step (c) is conducted repeatedly two to three times, the obtained mycelia are cultured to form mushrooms, and mature sporocarp is obtained; (e) the step (b) to the step (d) are conducted repeatedly two to four times, and the obtained sporocarp is the strains of the phlebopus portentosu. According to the phlebopus portentosu good character domestication method, the capability of the phlebopus portentosu for decomposing and utilizing lignin and cellulose is enhanced, the blending resisting capability is improved, and thus the fruiting rate, the fruiting uniformity and the maturing rate are improved; the biological efficiency of cultivation of the phlebopus portentosu is improved, and the characters of the obtained phlebopus portentosu are good.

Description

technical field [0001] The invention relates to the field of domestication of edible fungus strains, in particular to a method for domestication of black boletus with excellent traits. Background technique [0002] Black boletus (Phlebopus portentosu), also known as dark brown reticulate boletus, is mainly distributed in Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, New Zealand and my country's Yunnan, Guangxi and Hainan. It is a precious species in tropical areas. It is the most popular edible fungus among consumers, and it is also the only boletus that can be cultivated by saprophytic cultivation methods in the Boletaceae (Boletaceae) Phlebopus. Black boletus is fresh and tender in quality, delicious in taste, rich in nutritional value, high in protein and low in fat, rich in 17 kinds of essential amino acids and mineral elements necessary for human body such as phosphorus, potassium, calcium, magnesium, iron and zinc. Good health food and has good me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 石建同纪光燕郭细龙
Owner 贵州宏臻菌业投资发展有限公司
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