Uses of CHIP protein in pancreas cancer early stage diagnosis and prognosis determination

A technique for pancreatic cancer, use, and application in the field of serum markers

Inactive Publication Date: 2014-11-12
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, the expression level of CHIP in breast cancer, gastric cancer and brain glioma is significantly lower than that in adjacent normal tissues. These results all suggest that CHIP plays a tumor suppressor role in various tumors. There is no relevant report on the expression level of CHIP in serum at home and abroad

Method used

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  • Uses of CHIP protein in pancreas cancer early stage diagnosis and prognosis determination
  • Uses of CHIP protein in pancreas cancer early stage diagnosis and prognosis determination
  • Uses of CHIP protein in pancreas cancer early stage diagnosis and prognosis determination

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the collection of experimental material

[0030] 1. Histopathological specimens:

[0031] The specimens of pancreatic ductal adenocarcinoma confirmed by pathological examination were collected in Peking Union Medical College Hospital from January 2006 to July 2010, and the specimens were preserved in the Peking Union Medical College Hospital Basic Surgery -80°C Specimen Bank. The inclusion and exclusion criteria of the research sample are as follows:

[0032] 1) Inclusion criteria: pathological diagnosis of pancreatic ductal adenocarcinoma specimens, cases of surgical resection.

[0033] 2) Exclusion criteria: no tumor resection, or only pancreas tissue biopsy; incomplete case data, unable to obtain follow-up information; specimens could not be prepared into immunohistochemical sections.

[0034]A total of 58 patients with pancreatic cancer were included in this study, including 25 males and 33 females, with a male-to-female ratio of 1:1.32; 38 cases wer...

Embodiment 2

[0045] Embodiment 2, experimental method

[0046] Techniques well known in the art were used to extract the histoprotein, histoprotein SDS-PAGE and Western blot, immunohistochemical detection, and ELISA to detect the expression level of CHIP in human serum. The ELISA kit of CHIP protein was purchased from Wuhan Huamei Biological Co., Ltd., kit number CBS-EL022887HU.

[0047] The judgment of immunohistochemical results is as follows:

[0048] Divided into four grades according to the percentage of stained positive cells

[0049] -Negative

[0050] +<30% positive cell count

[0051] ++30-60% positive cell count

[0052] +++>60% positive cell count

[0053] The percentage of positive cells was 60%, divided into two groups with low expression of CHIP and high expression of CHIP. Scoring was done independently by two pathologists.

[0054] Statistical Analysis:

[0055] For enumeration data, Chi-square test or Fisher's exact test was used; for univariate analysis, Log-rank ...

Embodiment 3

[0056] Embodiment 3, experimental result

[0057] 1), SDS-PAGE and Western blot to detect the expression levels of CHIP and Hsp70 in pancreatic cancer and adjacent tissues

[0058] It was found that the expression level of CHIP in the pancreatic cancer tissues of the first and third patients was increased, and the expression level of CHIP was significantly decreased in the second and fourth patients. The expression level of Hsp70 was not significantly different, the expression level of the first and third patients increased, the expression level of the second patient had no significant difference, and the expression level of the fourth patient decreased. ( figure 1 )

[0059] 2) Immunohistochemical detection of the expression level of CHIP protein in pancreatic cancer tissues and adjacent tissues

[0060] The expression of CHIP protein is mainly in the cytoplasm, which is light yellow to dark brown. There were 37 cases with negative expression of CHIP protein in paracancer...

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Abstract

The invention relates to uses of a CHIP protein in pancreas cancer early stage diagnosis and prognosis determination, further provides a use of the CHIP protein in the preparation of kits for diagnosing pancreas cancer patients, and especially provides a use of the CHIP protein in the preparation of kits for assessing prognosis of the pancreas cancer patients.

Description

technical field [0001] The invention relates to the use of CHIP protein as a serum marker for diagnosing pancreatic cancer patients and evaluating the prognosis of pancreatic cancer patients. Background technique [0002] Pancreatic cancer is one of the most difficult to detect early, the most malignant and the worst prognosis tumors. According to the statistical results of the American Cancer Society on the incidence and mortality of tumors in the United States, pancreatic cancer accounts for the fourth cause of all tumor deaths, and the 5-year survival rate is below 5%. The main cause of death is that more than 50% of the patients were diagnosed at an advanced stage, the tumor had surrounded blood vessels or had distant metastasis, and they were resistant to traditional radiotherapy, chemotherapy or targeted therapy. Therefore, improving the early diagnosis rate of pancreatic cancer, inhibiting tumor growth and distant metastasis, and improving the therapeutic effect of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/561G01N33/57438G01N33/57488G01N33/68
Inventor 赵玉沛张太平王天笑由磊周立廖泉戴梦华舒红徐建威李建曹喆
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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