A solid-state electrochemiluminescence sensor for detecting mercury ions and its preparation method and application

An electrochemical and mercury ion technology, applied in the field of heavy metal detection, can solve the problem of no disclosure, and achieve the effects of stable signal, good reproducibility and high precision

Active Publication Date: 2016-06-08
乐陵市金山电器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are no reports on the use of N-(4-aminobutyl)-N-ethylisoluminol (ABEI) to detect mercury ion content in water using solid-state electrochemiluminescence.

Method used

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  • A solid-state electrochemiluminescence sensor for detecting mercury ions and its preparation method and application
  • A solid-state electrochemiluminescence sensor for detecting mercury ions and its preparation method and application
  • A solid-state electrochemiluminescence sensor for detecting mercury ions and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0042] A solid-state electrochemiluminescent sensor for detecting mercury ions, the sensor is surface-mounted electropolymerized ABEI, and then sequentially assembled with glutaraldehyde, DNA1, which can pass through T-Hg 2+ -T mismatch binding DNA2, biotin at the end of DNA2 and glassy carbon electrode of avidin, the specific preparation steps are as follows:

[0043] (1) Preparation of DNA1-immobilized glassy carbon electrode

[0044] a. Polish the glassy carbon electrode with a diameter of 3-5mm with 1.0μm, 0.3μm, and 0.05μm Al2O3 in sequence, then ultrasonically clean it with ethanol and water in sequence, rinse it with water, and dry it with nitrogen gas for later use;

[0045] b. Place the glassy carbon electrode obtained in step (a) in H containing N-(4-aminobutyl)-N-ethylisoluminol (ABEI) 2 SO 4 In the solution, carry out cyclic voltammetry scanning, the voltage range is -0.2~1.5V, with the scanning speed of 0.05V / s cycle scanning 20 circles, make ABEI electropolymer...

specific Embodiment 2

[0062] The method for detecting mercury ions using the solid-state electrochemiluminescence sensor prepared in the above specific embodiment two, the principle of detecting mercury ions is as follows figure 1 As shown, the specific steps are as follows:

[0063] a. After washing the glassy carbon electrode immobilized with DNA1 prepared in the above specific example 2 with 0.01-0.1MPBS buffer solution, start the electrode in 0.05-0.2M carbonate buffer solution with pH=9-10 Chemical reaction, test electrochemiluminescence intensity I 0 ;

[0064] b. Put the solid-state electrochemiluminescence sensor for mercury ion detection prepared in the above specific example 2 into 0.05-0.2M carbonate buffer solution with pH=9-10, start the electrochemical reaction, and measure the intensity of electrochemiluminescence , to obtain a series of different concentrations of Hg 2+ The corresponding electrochemiluminescence intensity value I of the solution 1 , to calculate a series of diff...

specific Embodiment 3

[0072] Highly selective and sensitive detection assay

[0073] High sensitivity is reflected by specific embodiment two, because the change value ΔI=I of electrochemiluminescence intensity 0 -I 1 , and the logarithm of the concentration of mercury ions is linear in the concentration range of 0.01 to 10nM. Therefore, the solid-state electrochemiluminescent sensor for detecting mercury ions prepared in the specific embodiment 1 is sensitive to Hg 2+ The detection sensitivity reaches 0.01nM.

[0074] High selectivity: with the same experimental conditions as in the above-mentioned specific example 2, detect common interfering ions of 10 μM: Pb 2+ ,Mn 2+ ,Co 2+ , Ni 2+ ,Cu 2+ ,Zn 2+ ,Cd 2+ ,Mg 2+ , the result is as Figure 4 shown.

[0075] The result shows: 0.01nMHg 2+ The ΔI of the sensor is about 800, and the ΔI of the sensor is less than 200 due to the common interfering ions of 10 μM, which means that 1000000 times of the common interfering ions do not affect the ...

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Abstract

The invention discloses a solid-state electrochemiluminescence sensor for mercury ion detection and its preparation method and application. -Hg 2+ -T mismatch binding DNA2, biotin at the end of DNA2 and glassy carbon electrode of avidin; its preparation method includes the preparation step of immobilized DNA1 glassy carbon electrode and the assembly step of solid-state electrochemiluminescence sensor, the advantage is that it has sensitivity High, good stability, strong selectivity, good reproducibility, easy operation, saving reagents, etc.

Description

technical field [0001] The invention relates to the technical field of heavy metal detection, in particular to a solid-state electrochemical luminescence sensor for detecting mercury ions, a preparation method and application thereof. Background technique [0002] Heavy metal pollution is one of the important components of environmental pollution. Mercury (Hg), the only liquid metal at room temperature, is the number one killer of heavy metal pollution. In recent years, the mining, smelting, processing and commercial manufacturing activities of heavy metal mercury have been increasing, and a large amount of mercury has entered the atmosphere, water and soil for retention, accumulation and migration. Mercury in the environment finally enters the body of animals and humans in the form of food chain, causing great damage to the vital functions of organisms, and causing diseases such as neuropsychiatric symptoms, tremors, stomatitis, and toxic nephropathy. Mercury ion (Hg 2+ ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/30
Inventor 郭智勇陈贝贝沙玉红徐成银郝婷婷
Owner 乐陵市金山电器有限公司
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