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Synchronous measurement method capable of meeting analytic accuracy of ADF and ADL in tobacco

A determination method and a technique of accuracy are applied in the field of simultaneous determination that satisfies the analysis accuracy of ADF and ADL in tobacco

Inactive Publication Date: 2014-12-03
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an analysis method that can greatly improve the analysis efficiency, save the analysis cost, and make the measured results meet the analysis deviation requirements of the industry while simultaneously measuring ADF and ADL in tobacco. Some analytical techniques cannot take into account the shortcomings of these two points

Method used

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  • Synchronous measurement method capable of meeting analytic accuracy of ADF and ADL in tobacco
  • Synchronous measurement method capable of meeting analytic accuracy of ADF and ADL in tobacco
  • Synchronous measurement method capable of meeting analytic accuracy of ADF and ADL in tobacco

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] Sample preparation and determination

[0037] A total of 13 samples with different ash content and origin were selected in the experiment, among which flue-cured tobacco and oriental tobacco were collected in Yunnan and Guizhou production areas, and burley tobacco was collected in Sichuan production area.

[0038] The determination of ADF and ADL refers to the method of YC / T 347-2010, each item sample is measured twice in parallel, and the average value is taken. Since the addition of n-octanol as a defoamer in the ISO 13906:2008 analysis method is 2-4 drops, while the industry standard addition is 1mL, it is found that the defoaming difference of the two additions is not obvious in the actual test. For the purpose of saving reagents, 2-4 drops were used in the experiment.

[0039] The ash content of the sample was determined according to the method of YC / T 427-2012.

[0040] Data Analysis and Results Analysis

[0041] Excel 2003 and SPSS10.0 were used for data...

Embodiment 1-13

[0042] Examples 1-13 ADF*om after ADF was corrected by the silicate content in ADL according to the above method, ADF*om: silicate content in ADF-ADL.

[0043]

[0044]

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Abstract

The invention discloses a synchronous measurement method capable of meeting analytic accuracy of ADF and ADL in tobacco. When ADF and ADL in tobacco are needed to be simultaneously measured by using a Van Soest washing method, an ADF result which is not subjected to ashing correction in the ADL analysis step is corrected by utilizing the content of silicate in ADL, so that the deviation between the measurement result of the ADF and cellulose in tobacco type samples of different ash contents and the measurement result obtained by ashing correction in the tobacco industry standard is controlled to be within 5 percent and meets or is higher than the deviation requirement of the conventional industrial standard, and the analysis step of singly measuring the ADF is omitted.

Description

technical field [0001] The invention relates to an analysis method capable of meeting the analysis deviation requirements of the industry and realizing simultaneous determination of ADF and ADL in tobacco, belonging to the technical field of methods for measuring ADF and ADL in tobacco. Background technique [0002] Cellulose, hemicellulose and lignin are the main substances constituting plant cell walls, which not only have a great impact on the quality of tobacco leaves, but also have great value in papermaking, feed, bioenergy, etc., so their determination methods have been It is the focus of research in many industries. [0003] In order to improve the analytical efficiency of simultaneous determination of acid detergent fiber (ADF) and acid-washed lignin (ADL) in tobacco, the tobacco industry currently adopts the Van Soest method developed by the United States Department of Agriculture in the 1960s ((YC / T 347 -2010, gravimetric method) is used as a standard analysis met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/00
Inventor 张婕耿召良葛永辉向章敏周淑平杨慧
Owner GUIZHOU TOBACCO SCI RES INST