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Light field microscopical method based on FPM

A technology of light field microscopy and light field microscopy, used in microscopes, optics, optical components, etc., can solve the problems of small magnification of observed objects and narrowed field of view, achieving less manual intervention, enriching light field information, and improving The effect of angular resolution

Active Publication Date: 2014-12-03
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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Problems solved by technology

Common optical imaging platforms are limited by the space bandwidth product, that is, if we observe a wider field of view, the magnification of the observed object will be small; on the contrary, if the magnification of the observed object is relatively large, then the range of the field of view will shrink

Method used

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  • Light field microscopical method based on FPM
  • Light field microscopical method based on FPM
  • Light field microscopical method based on FPM

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0019] Such as figure 1 As shown, some embodiments of the FPM-based light field microscopy method include the following steps:

[0020] S1. Build a light field microscope platform based on FPM. The FPM-based light field microscopy platform built here mainly includes two modules: one is the light field information acquisition module, and the other is the FPM-based image super-resolution reconstruction module.

[0021] The light field information collection module is also the light field microscope. The structure of the light field microscope is as figure 2 As shown, the optical path is as image 3 As shown, the working process is as follows Figure 4 shown. Compared with ordinary microscopes, light field microscopes place a microlens array at the original eyepiece position.

[0022] Such as Figure 5 As shown, the FPM-based image super-resolutio...

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Abstract

A light field microscopical method based on FPM includes the following steps of building a light field microscopical platform based on FPM, collecting high-definition wide-vision images through the light field microscopical platform based on FPM, carrying out view separation on the obtained high-definition wide-vision images, and obtaining wanted results through refocusing by means of results of view separation. Light field information is collected by means of a light field microscope based on an FPM algorithm, definition of each microlens image in the light field microscope is improved, angle definition is improved, the collected light field information is enriched, and a better three-dimensional structure can be reconstructed for an object.

Description

technical field [0001] The invention belongs to the fields of microscopic imaging, computer vision and computer graphics, especially the field of stereographics, and particularly relates to the technique of capturing and reconstructing microscopic light fields by using FPM algorithm. technical background [0002] Fourier ptychographic microscopy (FPM) is an image super-resolution reconstruction method based on a microscope platform, which can effectively overcome the contradictory problem of limited space bandwidth product. Common optical imaging platforms are limited by the space bandwidth product, that is, if we observe a wider field of view, the magnification of the observed object will be small; on the contrary, if the magnification of the observed object is relatively large, then the range of the field of view will shrink. The FPM algorithm solves this problem very well, allowing us to obtain images with a wide field of view and high resolution, thereby breaking throug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/36
Inventor 张永兵蒋伟鑫戴琼海
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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