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31 results about "Light field microscopy" patented technology

Light field microscopy (LFM) is a scanning-free 3-dimensional (3D) microscopic imaging method based on the theory of light field. This technique allows sub-second (~10 Hz) large volumetric imaging ([~0.1 to 1 mm] 3) with ~1 μm spatial resolution in the condition of weak scattering and semi-transparence,...

Light Field Microscope With Lenslet Array

ActiveUS20080180792A1Avoids mechanical complicationAberration correctionMicroscopesLensViewpointsImage plane
A light field microscope incorporating a lenslet array at or near the rear aperture of the objective lens. The microscope objective lens may be supplemented with an array of low power lenslets which may be located at or near the rear aperture of the objective lens, and which slightly modify the objective lens. The result is a new type of objective lens, or an addition to existing objective lenses. The lenslet array may include, for example, 9 to 100 lenslets (small, low-power lenses with long focal lengths) that generate a corresponding number of real images. Each lenslet creates a real image on the image plane, and each image corresponds to a different viewpoint or direction of the specimen. Angular information is recorded in relations or differences among the captured real images. To retrieve this angular information, one or more of various dense correspondence techniques may be used.
Owner:ADOBE SYST INC

Light field microscopical method based on FPM

A light field microscopical method based on FPM includes the following steps of building a light field microscopical platform based on FPM, collecting high-definition wide-vision images through the light field microscopical platform based on FPM, carrying out view separation on the obtained high-definition wide-vision images, and obtaining wanted results through refocusing by means of results of view separation. Light field information is collected by means of a light field microscope based on an FPM algorithm, definition of each microlens image in the light field microscope is improved, angle definition is improved, the collected light field information is enriched, and a better three-dimensional structure can be reconstructed for an object.
Owner:SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV

Frequency shift light field microscope and three-dimensional super-resolution microscopic display method

The invention discloses a frequency shift light field microscope, and the microscope comprises an objective table, an illuminating module, a microscopic module, an image obtaining module, and an image processing module. The illuminating module is arranged below the objective table, and can irradiate a sample at different angles. The image obtaining module obtains the light field images which are formed by the irradiation of the sample from different angles. The image processing module obtains all depth images through the refocusing of the light field images, carries out the frequency spectrum iteration after all images at each depth are obtained, carries out the filtering of the obtained high-resolution images, and then carries out the three-dimensional reconstruction through the high-resolution images at different depths. The invention also discloses a three-dimensional super-resolution microscopic display method. The microscope and the display method combine the technology of frequency spectrum iteration and the technology of light fields together, improve the imaging depth and resolution, and can recover a better microscopic three-dimensional image with the depth.
Owner:ZHEJIANG UNIV

Enhancing the resolution of three dimensional video images formed using a light field microscope

Methods and systems are provided for enhancing the imaging resolution of three dimensional imaging using light field microscopy. A first approach enhances the imaging resolution by modeling the scattering of light that occurs in an imaging target, and using the model to account for the effects of light scattering when de-convolving the light field information to retrieve the 3-dimensional (3D) volumetric information of the imaging target. A second approach enhances the imaging resolution by using a second imaging modality such as two-photon, multi-photon, or confocal excitation microscopy to determine the locations of individual neurons, and using the known neuron locations to enhance the extraction of time series signals from the light field microscopy data.
Owner:UNIVERSITY OF VIENNA

System for rapid assessment of water quality and harmful algal bloom toxins

The present invention is directed toward the early detection of harmful algal blooms. The system employs the ability of whole cell non-contact micro Raman spectroscopy to detect cell pigmentation in such a way that distinct patterns or fingerprints can be assembled. Light field microscopy will provide a fundamentally innovative increase in image and sample volume. Furthermore, darkfield microscopy is employed to capture high-resolution, color images of the detected plankton to increase the accuracy of species identification and classification. Together, this new instrument will provide a powerful yet elegantly simple solution to detection of HAB cells and characterization of environmental conditions.
Owner:WOODS HOLE OCEANOGRAPHIC INSTITUTION

Tomographic Light Field Microscope

An optical tomography system includes a light field microscope including an objective lens, a computer-controlled light source, a condenser lens assembly and a microlens array aligned along an optical axis. A carrier containing a specimen is coupled to a rotational driver for presenting varying angles of view of the specimen. A photosensor array disposed to receive photons from the objective lens. A computer is linked to control the computer-controlled light source and condenser lens assembly and the rotational driver, and coupled to receive images from the photosensor array where the light field microscope simultaneously captures a continuum of focal planes in the specimen for each of a set of the varying angles of view of the specimen.
Owner:VISIONGATE

Light-field microscope with selective-plane illumination

A light-field microscope includes a light-sheet focusing device configured for outputting illumination light as a light sheet, and a microlens array between an objective lens and a light detector. A sample is irradiated by the light sheet along a direction non-parallel to the sample plane. Light-field data may be acquired from the sample without needing to scan through the thickness of the sample.The microscope implements light-field acquisition in conjunction with selective plane illumination.
Owner:MOLECULAR DEVICES AUSTRIA GMBH

Optical field microscopic system, optical field microscope and optical module thereof

The invention provides an optical module used for an optical field microscope. The optical module comprises a microscopic objective and a microlens array arranged on a back focal plane of the microscopic objective, wherein the microlens array comprises a plurality of microlenses, the plurality microlenses are used for projecting real images of a sample under the microscope objective at different viewing angles onto a sensor located on an image plane of the optical field microscope, the plurality of microlenses are divided into at least two microlens groups, all the microlenses in each microlens group have the same optical parameters and axial positions, and the different microlens groups are configured to optically conjugate object planes under the microscope objective at different axial positions to the image plane on which the sensor is located.
Owner:CENT FOR EXCELLENCE IN BRAIN SCI & INTELLIGENCE TECH CHINESE ACAD OF SCI

Single-tube optical field microscope

InactiveCN107219620AResolve depth of fieldSolve the contradiction of magnificationMicroscopesCMOSSingle exposure
The invention discloses a single-tube optical field microscope used for refocusing imaging and three-dimensional imaging after single exposure of a sample. The single-tube optical field microscope adopts a far field correction objective, and a microlens array and a CMOS (Complementary Metal Oxide Semiconductor) imaging device are inserted into a primary image surface and combined into an optical field sensor to acquire an optical field. Refocusing imaging and three-dimensional imaging are performed on the sample via primary exposure acquisition, optical field recording and imaging calculation. By sufficiently using the characteristic of the microlens array and using the two-dimensional sensor to record four-dimensional optical field information, the depth-of-field problem of the microscope is solved, and mechanical fine focusing is not needed for acquiring a plane image of an object. The single-tube optical field microscope is simple in structure and flexible in application scenes. A target three-dimensional image can be obtained by inverting the acquired four-dimensional optical field information.
Owner:INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI

Light sheet illumination-based high-resolution four-dimensional light field microscopy imaging system

The invention discloses a light sheet illumination-based high-resolution four-dimensional light field microscopy imaging system. The system comprises a light sheet illumination module, a motion control module and a detection and acquisition module, wherein the light sheet illumination module is used for generating an intensity-adjustable wavelength-adjustable and thickness-adjustable Gaussian light sheet for tomographic illumination of a to-be-observed sample; the motion control module is used for realizing the motion of the to-be-observed sample in a three-dimensional space; and the detectionand acquisition module is used for acquiring signals from the to-be-observed sample, carrying out light field detection on the to-be-observed sample and recording and storing the acquired signals byutilizing an image sensor. The four-dimensional microscopy imaging system disclosed by the invention has the beneficial effects that the advantages of the light sheet illumination and the light fielddetection are combined, so that the difficulty of observing a three-dimensional living body motion sample during the microscopy imaging can be effectively solved and high frame rate and high time resolution are realized without sacrificing the spatial resolution at the same time.
Owner:HUAZHONG UNIV OF SCI & TECH

Reconstruction method for microscopic light field body imaging and forward process and back projection acquisition method thereof

ActiveCN110989154AImprove rebuild speedImprove convolution calculation speedMicroscopesComputer graphics (images)Point spread function
The invention discloses a reconstruction method for a microscopic light field body imaging and forward process and back projection acquisition method thereof, and the method comprises the steps: step1, decomposing a 3D body image into layer images, wherein each layer image is composed of a plurality of 2D sub-images, and the pixel value of each 2D sub-image except coordinates (i, j) is zero; step2, extracting pixels at the coordinates (i, j) on each layer image so as to rearrange the pixels into single-channel images, and stacking all the single-channel images into a multi-channel image; step 3, rearranging the 5D point spread function of the light field microscope into a 4D convolution kernel; step 4, taking the multi-channel image obtained in the step 2 as network input of a convolutional neural network, taking the 4D convolution kernel obtained in the step 3 as a weight of the convolutional neural network, carrying out convolution on the multi-channel image by adopting the 4D convolution kernel, and outputting a multi-channel image; and step 5, rearranging the multi-channel image output in the step 4 into a light field image. The convolution calculation speed can be greatly improved, so that the image reconstruction speed is improved.
Owner:PEKING UNIV

Optical field microscopic system, optical field microscope and optical module thereof

The invention provides an optical module used for an optical field microscope. The optical module comprises a microscopic objective and a microlens array arranged on a back focal plane of the microscopic objective, wherein the microlens array comprises a plurality of microlenses, the plurality microlenses are used for projecting real images of a sample under the microscope objective at different viewing angles onto a sensor located on an image plane of the optical field microscope, gaps are reserved between the plurality of microlenses, and the gaps are filled with a light shielding material.
Owner:CENT FOR EXCELLENCE IN BRAIN SCI & INTELLIGENCE TECH CHINESE ACAD OF SCI

Ophthalmic surgery using light-field microscopy

A system and method for ophthalmic surgery in which a light field camera is used to capture a digital image of the surgical field including the eye. The digital image is used to create image information and directional information, which is then used to from a three dimensional (3D) image with motion parallax.
Owner:NOVARTIS AG

System for rapid assessment of water quality and harmful algal bloom toxins

The present invention is directed toward the early detection of harmful algal blooms. The system employs the ability of whole cell non-contact micro Raman spectroscopy to detect cell pigmentation in such a way that distinct patterns or fingerprints can be assembled. Light field microscopy will provide a fundamentally innovative increase in image and sample volume. Furthermore, darkfield microscopy is employed to capture high-resolution, color images of the detected plankton to increase the accuracy of species identification and classification. Together, this new instrument will provide a powerful yet elegantly simple solution to detection of HAB cells and characterization of environmental conditions.
Owner:WOODS HOLE OCEANOGRAPHIC INSTITUTION

Light field microscopic three-dimensional reconstruction method and device based on deep learning algorithm

The invention discloses a light-field microscope three-dimensional reconstruction method and device based on a deep learning algorithm, wherein the method comprises: building a light-field microscope imaging system; obtaining the point diffusion of the imaging system by simulating the light-field microscope imaging system function; generate a simulated sample volume distribution data set; generate a simulated light field data set through the point spread function and the corresponding sample data, and correct the possible noise and background model; build a deep convolutional neural network to simulate the light field data As the input of the network, the simulated sample body distribution data is used as the output of the network, and the network is trained according to the microscopic sample design loss function; after the model training is completed, the light field microscopic data to be reconstructed is input into the model for testing. Obtain the predicted value of the corresponding sample body distribution data. The method can achieve fast, high-resolution, and less artifact three-dimensional reconstruction of light field data while maintaining the advantage of the light field for fast collection of three-dimensional information.
Owner:TSINGHUA UNIV

Optical field microimaging-based micro-scale three dimensional industrial detection system

An optical field microimaging-based micro-scale three dimensional industrial detection system belonging to the machine vision technology field disclosed by the present invention comprises an optical field microcamera system and a data processing system, the optical field microcamera system is composed of an optical field microcamera, an annular light source and a camera calibration plate, and thedata processing system comprises a GPU high-speed image parallel processing unit, an optical field camera-based depth estimation algorithm and an optical filed microscope-based three-dimensional reconstruction algorithm. An optical field imaging technology of the present invention can measure the three-dimensional configurations of the complex objects via a single device and the individual shooting, is very suitable for substituting for a conventional industrial detection camera in the micro-scale measurement, and measures the three-dimensional sizes of the micro-scale objects while simplifying a hardware system and reducing the cost.
Owner:广州首感光电科技有限公司

Microscope imaging optical system and light-field microscope apparatus

A microscope imaging optical system of the present invention has an entrance pupil on the object side and includes, in order from the object side, a first lens group that is composed of biconvex lens and negative lens having a concave surface on the object side, a second lens group that is composed of negative lens having a concave surface on the object side, and a third lens group that is composed of meniscus lens and a positive lens having a convex surface on the image side. The microscope imaging optical system satisfies conditional expressions 0.05<dp1 / f<0.15 and 0.5<TT / f<0.9. dp1 is the spacing between the lens, closest to the object, of the first lens group and the entrance pupil, f is the focal length of the microscope imaging optical system, and TT is the distance from the entrance pupil to the object image plane.
Owner:OLYMPUS CORP

Optical-field microscopic system based on aspheric micro-lens array

InactiveCN108279493AStructural modificationEasy to transformMicroscopesImaging qualityImage resolution
The invention discloses an optical-field microscopic system based on an aspheric micro-lens array. The optical-field microscopic system comprises a micro-objective, the micro-lens array, a photosensitive element and a computer processing device, wherein the micro-lens array is an aspheric micro-lens array. According to the system, a spherical micro-lens array in a traditional optical-field microscope is changed into aspheric micro-lens array for correcting aberration, imaging quality of each sub lens can be improved, so that the resolution ratio of a reconstructed three-dimensional image and imaging quality are improved on the whole, and the accuracy of a reconstructed model of an optical-field microscope is improved.
Owner:FUDAN UNIV

A Microscopic Method and Device Based on Wide-field Stimulated Emission Differential

The invention discloses a microscopic method based on wide field stimulated emission difference. The method comprises the steps of firstly, carrying out wide field illumination and image formation on a sample, and then forming two beams of vertically polarized light (s light) and two parallel polarized light (p light) by utilizing laser and through two Wollaston prisms; carrying out illumination and image formation on the fluorescent sample by the interference light spot formed by line polarization focus of the four beams of light; carrying out differential treatment on the image obtained under the wide field and the image obtained under the interference light spot like a field emission display (FED), wherein the size of the dark spot is smaller than the diffraction limit, so that the image can be obtained in the dark spot by super-resolution; finally, controlling the interference light spot to move on the sample by a scanning galvanometer to obtain the whole microscopic image of the sample. The invention also discloses a microscopic device based on wide field stimulated emission difference; the device is simple in structure and convenient to operate, and rapid super-resolution microscopic imaging based on wide field stimulated emission difference can be realized, so that the device can be used for the field of optical microscopic imaging.
Owner:ZHEJIANG UNIV

Microscope imaging optical system and light-field microscope including the microscope imaging optical system

A microscope imaging optical system has an entrance pupil on an object side and includes, in order from the object side, a first lens group including a biconvex lens and a negative lens having a concave surface on the object side, a second lens group including a negative lens having a concave surface on the object side, and a third lens group including a meniscus lens and a positive lens having a convex surface on an image side. The microscope imaging optical system satisfies conditional expressions 0.05<dp1 / f<0.15 and 0.5<TT / f<0.9, where dp1 is the spacing between a lens, closest to the object, of the first lens group and the entrance pupil, f is the focal length of the microscope imaging optical system, and TT is the distance from the entrance pupil to the object image plane.
Owner:OLYMPUS CORP

Light field microscopy system and method based on front micro lens array

The invention discloses a light field microscopy system and method based on a front micro lens array, the system comprises a micro lens array module, a microscopy optical amplification module and a detection module, the micro lens array module is used for encoding input space three-dimensional light field distribution into two-dimensional light field distribution of a front focal plane of a microobjective; the micro-optical amplification module is connected with the micro-lens array module and is used for carrying out spatial transformation on the two-dimensional light field distribution to obtain an optical signal; and the detection module is connected with the microscopic optical amplification module and is used for receiving an optical signal output by the microscopic optical amplification module and generating an information processing result. According to the system, the performance of a traditional optical microscope is improved such that the traditional optical microscope can realize the high-speed observation of a three-dimensional sample with any surface shape.
Owner:TSINGHUA UNIV

Microscope and method for light field microscopy and confocal microscopy with light sheet excitation

The invention relates to a microscope comprising an excitation beam path in which are present: a light source; an objective lens that illuminates the sample with excitation radiation and captures detection radiation; and means for switching between the first and second operating modes of the microscope. A color separator is present in the excitation beam path, which is reflective to the excitation radiation, the beam of excitation radiation being reflected along another excitation beam path due to the action of the color separator. Furthermore, a first device is present in the excitation beam path between the light source and the color separator, by means of which the first cylindrical optical element can be introduced into the excitation beam path in a first operating state of the microscope; a second device is present in the excitation beam path between the optical splitter and the scanning device, by means of which the second cylindrical optical element can be introduced into the excitation beam path in the first operating state of the microscope. The invention also relates to a method of operating a microscope having two operating modes.
Owner:CARL ZEISS MICROSCOPY GMBH

Reconstruction method of microscopic light field volume imaging and its forward process and back projection acquisition method

ActiveCN110989154BImprove rebuild speedImprove convolution calculation speedMicroscopesPoint spread functionBack projection
The invention discloses a method for reconstructing volumetric imaging in a microscopic light field and its forward process and back-projection acquisition method. Composed of 2D sub-images, the pixel value of each 2D sub-image is zero except for coordinates (i, j); step 2, extract the pixels at coordinates (i, j) on each layer image to rearrange into a single channel image, stack all single-channel images into multi-channel images; step 3, rearrange the 5D point spread function of the light field microscope into a 4D convolution kernel; step 4, use the multi-channel images obtained in step 2 as the convolutional neural network Network input, use the 4D convolution kernel obtained in step 3 as the weight of the convolutional neural network, use the 4D convolution kernel to convolve the multi-channel image, and output the multi-channel image; step 5, rearrange the multi-channel image output in step 4 is the light field image. The invention can greatly increase the convolution calculation speed, thereby improving the image reconstruction speed.
Owner:PEKING UNIV

Light field microscope system, light field microscope and optical components thereof

The invention provides an optical module used for an optical field microscope. The optical module comprises a microscopic objective and a microlens array arranged on a back focal plane of the microscopic objective, wherein the microlens array comprises a plurality of microlenses, the plurality microlenses are used for projecting real images of a sample under the microscope objective at different viewing angles onto a sensor located on an image plane of the optical field microscope, gaps are reserved between the plurality of microlenses, and the gaps are filled with a light shielding material.
Owner:CENT FOR EXCELLENCE IN BRAIN SCI & INTELLIGENCE TECH CHINESE ACAD OF SCI
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