The invention discloses a reconstruction method for a microscopic light field body imaging and forward process and back projection acquisition method thereof, and the method comprises the steps: step1, decomposing a 3D body image into layer images, wherein each layer image is composed of a plurality of 2D sub-images, and the pixel value of each 2D sub-image except coordinates (i, j) is zero; step2, extracting pixels at the coordinates (i, j) on each layer image so as to rearrange the pixels into single-channel images, and stacking all the single-channel images into a multi-channel image; step 3, rearranging the 5D point spread function of the light field microscope into a 4D convolution kernel; step 4, taking the multi-channel image obtained in the step 2 as network input of a convolutional neural network, taking the 4D convolution kernel obtained in the step 3 as a weight of the convolutional neural network, carrying out convolution on the multi-channel image by adopting the 4D convolution kernel, and outputting a multi-channel image; and step 5, rearranging the multi-channel image output in the step 4 into a light field image. The convolution calculation speed can be greatly improved, so that the image reconstruction speed is improved.