A Microscopic Method and Device Based on Wide-field Stimulated Emission Differential
A stimulated emission, wide-field technology, applied in the field of super-resolution microscopy, can solve the problem of reducing the effective signal light luminous area, etc., and achieve the effects of easy operation, convenient data processing, and simple device structure
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Embodiment 1
[0039] Such as figure 1 As shown, a microscope device based on wide-field stimulated emission difference for fluorescent samples, including a second laser 1, a first Wollaston prism 2, a second Wollaston prism 3, and a second lens 4, The first dichroic mirror 5, the second dichromatic mirror 6, the scanning vibrating mirror 7, the objective lens 9, the sample 10, the sample stage 11, the first lens 12, the first laser 13, the filter 14, the detector 15, and the computer 16.
[0040] use figure 1 The setup shown implements a wide-field stimulated emission difference-based microscopy method for fluorescent samples, which proceeds as follows:
[0041] (1) The first laser 13 emits laser light, which is collimated by the first lens 12 to form collimated light. The collimated light is completely reflected by the first dichroic mirror 5, then completely transmitted by the second dichroic mirror 6, and then passed through the scanning galvanometer 7 reflection, and finally focused...
Embodiment 2
[0048] Such as figure 1 As shown, a microscope device based on wide-field stimulated emission difference under total internal angle reflection fluorescence (TIRF) for fluorescent samples, including a second laser 1, a first Wollaston prism 2, a second Wollaston Prism 3, second lens 4, first dichroic mirror 5, second dichroic mirror 6, scanning galvanometer 7, objective lens 9, sample 10, first lens 12, first laser 13, filter 14, detector 15, computer 16.
[0049] use figure 1 The shown setup implements a wide-field stimulated emission difference-based microscopy method under total internal angle reflection fluorescence (TIRF) for fluorescent samples, and the procedure is as follows:
[0050] (1) The first laser 13 emits laser light, which is collimated by the first lens 12 to form collimated light. The collimated light is completely reflected by the first dichroic mirror 5, then completely transmitted by the second dichroic mirror 6, and then passed through the scanning ga...
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