Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Three-dimensional microscopy imaging method and system based on light field microscopy system

A technology of microscopic imaging and light field microscopy, which is applied in the field of computational photography and can solve the problems of difficulty in improving the reconstruction resolution at the focal plane and sparse spatial distribution at the imaging focal plane.

Active Publication Date: 2022-05-20
TSINGHUA UNIV
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is limited by the sparse spatial distribution at the imaging focal plane, making it difficult to improve the reconstruction resolution at the focal plane

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Three-dimensional microscopy imaging method and system based on light field microscopy system
  • Three-dimensional microscopy imaging method and system based on light field microscopy system
  • Three-dimensional microscopy imaging method and system based on light field microscopy system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0050] Light field microscopy technology is a fast three-dimensional microscopy technology proposed in recent years. It extends the characteristics of traditional microscopy technology to collect spatial information to the simultaneous collection of spatial information and angle information, so that a four-dimensional light field can be obtained by one acquisition. Information, including two-dimensional spatial information and two-dimensional angle information, further realizes three-dimensional imaging. The specific method is: i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a three-dimensional microscopic imaging method and system based on a light field microscopic system, wherein the method includes: S1, in the optical system, acquiring the first point diffusion of the three-dimensional sample from the object plane to the sensor plane of the main camera function and the second point spread function from the object surface to the secondary camera sensor plane, generate the first forward projection matrix and the second forward projection matrix according to the first point spread function and the second point spread function; S2, acquire the three-dimensional sample in The light field intensity image taken by the main camera in the optical system and the high-resolution intensity image taken by the secondary camera; S3, the light field intensity image and the first forward projection matrix, the high-resolution intensity image and the second The forward projection matrix performs 3D reconstruction to generate a 3D reconstruction result of the 3D sample. In this method, by adding one acquisition optical path, the signal-to-noise ratio of focal plane reconstruction is enhanced under the same number of iterations, and the reconstruction effect of light field microscopic imaging is greatly improved.

Description

technical field [0001] The invention relates to the technical field of computational imaging, in particular to a three-dimensional microscopic imaging method and system based on a light field microscopic system. Background technique [0002] Usually, the microscopic samples that need to be observed are often three-dimensional. In traditional microscopic techniques, only one depth of the sample can be imaged two-dimensionally, and the sample slice can be observed, but the three-dimensional structure of the sample cannot be observed, which cannot meet the needs of some biological fields. If it is necessary to collect data of three-dimensional samples, it is necessary to collect multiple images of different depths through longitudinal axis scanning and other methods, and then perform axial splicing. This method is relatively slow. In the case of high dynamics, the scanning speed of the vertical axis Below the dynamic change rate of biological structures, it is difficult to ima...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/557G06T11/00G06T15/00G02B21/36
CPCG02B21/367G06T7/557G06T11/003G06T15/00H04N13/232H04N13/204G02B21/22
Inventor 戴琼海卢志吴嘉敏
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products