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fpm-based light-field microscopy

A technology of light field microscopy and light field microscopy, which is applied in the direction of microscopes, optics, optical components, etc., can solve the problems of small magnification of observed objects and narrow field of view, so as to achieve less manual intervention, enrich light field information, and improve Effect of angular resolution

Active Publication Date: 2016-08-17
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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Problems solved by technology

Common optical imaging platforms are limited by the space bandwidth product, that is, if we observe a wider field of view, the magnification of the observed object will be small; on the contrary, if the magnification of the observed object is relatively large, then the range of the field of view will shrink

Method used

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0019] Such as figure 1 As shown, some embodiments of the FPM-based light field microscopy method include the following steps:

[0020] S1. Build a light field microscope platform based on FPM. The FPM-based light field microscopy platform built here mainly includes two modules: one is the light field information acquisition module, and the other is the FPM-based image super-resolution reconstruction module.

[0021] The light field information collection module is also the light field microscope. The structure of the light field microscope is as figure 2 As shown, the optical path is as image 3 As shown, the working process is as follows Figure 4 shown. Compared with ordinary microscopes, light field microscopes place a microlens array at the original eyepiece position.

[0022] Such as Figure 5 As shown, the FPM-based image super-resolutio...

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Abstract

A light field microscopy method based on FPM, comprising the steps of: building a light field microscopy platform based on FPM; collecting images with high resolution and wide field of view with the light field microscopy platform based on FPM; The field of view image is separated from the view angle; the result of the view separation is used to refocus to obtain the desired result. The present invention uses a light field microscope based on the FPM algorithm to collect light field information, improves the imaging resolution of each microlens in the light field microscope, improves the angular resolution, thereby enriches the collected light field information, and enables objects to Rebuild better 3D structures.

Description

technical field [0001] The invention belongs to the fields of microscopic imaging, computer vision and computer graphics, especially the field of stereographics, and particularly relates to the technique of capturing and reconstructing microscopic light fields by using FPM algorithm. technical background [0002] Fourier ptychographic microscopy (FPM) is an image super-resolution reconstruction method based on a microscope platform, which can effectively overcome the contradictory problem of limited space bandwidth product. Common optical imaging platforms are limited by the space bandwidth product, that is, if we observe a wider field of view, the magnification of the observed object will be small; on the contrary, if the magnification of the observed object is relatively large, then the range of the field of view will shrink. The FPM algorithm solves this problem very well, allowing us to obtain images with a wide field of view and high resolution, thereby breaking throug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/36
Inventor 张永兵蒋伟鑫戴琼海
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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