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Light sheet illumination-based high-resolution four-dimensional light field microscopy imaging system

A four-dimensional light field and microscopic imaging technology, which is applied in the fields of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of the sacrifice of spatial resolution in light field imaging, and achieve the goal of avoiding spatial resolution, avoiding waste, and high temporal resolution Effect

Active Publication Date: 2019-04-09
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the characteristics of high time resolution are destined to cause the spatial resolution of light field imaging to be sacrificed to a certain extent compared with traditional imaging systems.

Method used

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  • Light sheet illumination-based high-resolution four-dimensional light field microscopy imaging system
  • Light sheet illumination-based high-resolution four-dimensional light field microscopy imaging system
  • Light sheet illumination-based high-resolution four-dimensional light field microscopy imaging system

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Embodiment 1

[0048] In this embodiment, the system is mainly used for nematode motion imaging and three-dimensional reconstruction. Nematode is a commonly used model organism in biology. It is of great significance to study the movement of nematodes and the changes of neural signals during the corresponding movement. Using a commonly used wide-field microscope can capture the movement of nematodes very well, but the resolution and signal-to-noise ratio are low, which cannot meet the resolution requirements. Observation by light-sheet fluorescence microscopy, although better resolution and signal-to-noise ratio can be obtained, but three-dimensional fluorescence images of the movement process cannot be obtained. The use of this system can meet the needs of spatial resolution and time resolution of nematode motion imaging at the same time, and it is a useful tool for the research of related biological problems.

[0049] image 3 is the result of imaging nematodes in which pan neuron neuron...

Embodiment 2

[0052] In this embodiment, the system is mainly used for imaging and three-dimensional reconstruction of living zebrafish heart beating. The zebrafish heart is of great significance in the research of cardiac science and medicine. The study of cardiomyocyte movement and cardiac blood flow has important implications for the corresponding fields of medicine and pathology. Observation using a commonly used wide-field microscope, because the zebrafish heart is located in the body, there is tissue scattering and absorption, so the loss of resolution is large, and the observation at the cell level cannot be achieved. Observation using a light-sheet fluorescence microscope can only observe and record fluorescence signals at a certain depth, and cannot record and reconstruct the three-dimensional beating process of the entire heart. This system can solve this problem very well. Without loss of resolution, it can record and reconstruct the beating process of the zebrafish heart, which ...

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Abstract

The invention discloses a light sheet illumination-based high-resolution four-dimensional light field microscopy imaging system. The system comprises a light sheet illumination module, a motion control module and a detection and acquisition module, wherein the light sheet illumination module is used for generating an intensity-adjustable wavelength-adjustable and thickness-adjustable Gaussian light sheet for tomographic illumination of a to-be-observed sample; the motion control module is used for realizing the motion of the to-be-observed sample in a three-dimensional space; and the detectionand acquisition module is used for acquiring signals from the to-be-observed sample, carrying out light field detection on the to-be-observed sample and recording and storing the acquired signals byutilizing an image sensor. The four-dimensional microscopy imaging system disclosed by the invention has the beneficial effects that the advantages of the light sheet illumination and the light fielddetection are combined, so that the difficulty of observing a three-dimensional living body motion sample during the microscopy imaging can be effectively solved and high frame rate and high time resolution are realized without sacrificing the spatial resolution at the same time.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and more specifically relates to a high-resolution four-dimensional light field microscopic imaging system based on light sheet illumination, which is a three-dimensional microscopic imaging system with high frame rate and high signal-to-noise ratio , especially suitable for real-time three-dimensional observation and imaging of moving samples. Background technique [0002] Light-sheet fluorescence microscopy is a new type of microscopic imaging technology emerging in this century. Different from the traditional fluorescence microscope, the light sheet fluorescence microscope adopts the tomographic illumination method, that is, the illumination light source and the detection collection are perpendicular to each other, and a sheet-shaped light source is used to excite the fluorescence of the sample from the side, and a wide field is used to collect the excitation of the illumination layer. Fluo...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 费鹏杨一聪汪兆强
Owner HUAZHONG UNIV OF SCI & TECH
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