A complex antistaling agent and its application in fruit and vegetable preservation
The technology of a preservative and a complex compound is applied to the complex preservative and its application field in the preservation of fruits and vegetables, and can solve the problems of moisture retention, antibacterial defects, weak water resistance, and no antibacterial film in the film. Excellent film-forming and moisturizing properties, slowing down the loss of water, reducing release and breathing intensity
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Embodiment 1
[0042] Preparation of pullulan:
[0043] Pick a single colony of Aureobasidium tumefaciens (CCTCC M 2013611) and inoculate it in the fermentation basal medium, culture it on a shaker at 20°C for 5 days to obtain the fermentation broth;
[0044] Fermentation basal medium composition (g / L): potato 100, glucose 40;
[0045] Centrifuge the fermentation broth, take the supernatant and add 3 times the volume of ethanol to precipitate at 10°C for 10 hours, centrifuge, and then re-precipitate the obtained supernatant with 5 times the volume of ethanol, centrifuge, and dry at 50°C to constant weight to obtain the crude extract sample;
[0046] The crude sample was dissolved in distilled water, purified on a silica gel column to obtain a purified sample;
[0047] The purified sample was dissolved in distilled water and spray-dried to obtain pullulan powder.
[0048] Preparation of γ-polyglutamic acid:
[0049] Weigh the basal medium and dissolve it to obtain the basal medium solutio...
Embodiment 2
[0064] Preparation of pullulan:
[0065] Pick a single colony of Aureobasidium tumefaciens (CCTCC M 2013611) and inoculate it in the fermentation basal medium, and cultivate it on a shaker at 25°C for 4 days to obtain the fermentation broth;
[0066] Fermentation basal medium composition (g / L): potatoes 150, glucose 35;
[0067] Centrifuge the fermentation broth, take the supernatant and add 4 times the volume of ethanol to precipitate at 8°C for 12 hours, centrifuge, and re-precipitate the obtained supernatant with 4.5 times the volume of ethanol, centrifuge, and dry at 55°C to constant weight to obtain the crude extract sample;
[0068] The crude sample was dissolved in distilled water, purified on a silica gel column to obtain a purified sample;
[0069] The purified sample was dissolved in distilled water and spray-dried to obtain pullulan powder.
[0070] Preparation of γ-polyglutamic acid:
[0071] Weigh the basal medium and dissolve it to obtain the basal medium sol...
Embodiment 3
[0086] Preparation of pullulan:
[0087] Pick a single colony of Aureobasidium tumefaciens (CCTCC M 2013611) and inoculate it in the fermentation basal medium, culture it on a shaker at 30°C for 3 days to obtain the fermentation broth;
[0088] Components of fermentation basic medium (g / L): potatoes 200, glucose 30;
[0089] Centrifuge the fermentation broth, take the supernatant and add 5 times the volume of ethanol to precipitate at 5°C for 15 hours, centrifuge, and then re-precipitate the obtained supernatant with 4 times the volume of ethanol, centrifuge, and dry at 55°C to constant weight to obtain the crude extract sample;
[0090] The crude sample was dissolved in distilled water, purified on a silica gel column to obtain a purified sample;
[0091] The purified sample was dissolved in distilled water and spray-dried to obtain pullulan powder.
[0092] Preparation of γ-polyglutamic acid:
[0093] Weigh the basal medium and dissolve it to obtain the basal medium soluti...
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Abstract
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