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Application of lithium-containing natural material bioactive scaffold in osteochondral defect repair

A natural material and bioactive technology, applied in the field of application of lithium-containing natural material bioactive scaffolds in the repair of osteochondral defects, to achieve the effect of promoting bone and cartilage regeneration

Active Publication Date: 2014-12-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, scaffold materials have been widely used in the basic research of osteochondral defects, but the existing scaffold materials can only meet the pathophysiological ne

Method used

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  • Application of lithium-containing natural material bioactive scaffold in osteochondral defect repair
  • Application of lithium-containing natural material bioactive scaffold in osteochondral defect repair
  • Application of lithium-containing natural material bioactive scaffold in osteochondral defect repair

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0051] Example 1 Preparation of scaffold:

[0052] (1) Preparation of lithium-containing bioactive glass stent: 5.8g P123, 10g ethyl orthosilicate, 1.5g calcium nitrate tetrahydrate, 0.125g lithium chloride and 1.10g ethyl triphosphate were added to 110ml absolute ethanol, And adjust the pH to 6.5-7.0 with 0.5M hydrochloric acid, and stir at room temperature (37°C) for 24h. Completely immerse the sterile polyurethane sponge (25ppi) in the above solution for 10 minutes, then transfer it to a cell culture dish and place it at room temperature (37°C) for 12 hours, repeat the above process (immersion-place) 3 times, and then sinter at 650°C The organic template was removed for 6 hours to construct a lithium-containing bioactive glass scaffold. See the multiple scanning electron micrograph figure 2 Shown.

[0053] (2) The method of purely bioactive glass scaffold is the same as above, and the purely bioactive glass framework can be obtained by simply removing lithium chloride. See t...

Example Embodiment

[0058] Example 2 Lithium-containing biologically active silk fibroin scaffold

[0059] (1) Preparation of silk fibroin solution: a) Degumming: Put 100g of mulberry silk (Zhejiang Huazhi Silk Co., Ltd., grade 5A) into 5L of 2M sodium carbonate aqueous solution, bath at 96℃ for 30min, and wash with purified water. This process is repeated 3 times to remove the sericin and leave the silk fibroin. The silk fibroin is dried at 50°C to obtain 70g of dried silk fibroin for use; b) dissolution: the above-mentioned dried silk fibroin The protein is dissolved in a 9.3M lithium bromide (LiBr) aqueous solution with a mass-volume ratio of 0.2:1, and the silk fibroin is fully dissolved in a 60°C water bath for 90 minutes to obtain a mixture containing silk fibroin and a small amount of insoluble particles; c) dialysis: The mixed solution was dialyzed with a regenerated cellulose dialysis bag (with a cut-off molecular weight of 4000 Daltons), and then dialyzed with 10 times the volume of the mi...

Example Embodiment

[0066] Example 3

[0067] 1. Animal model establishment and stent implantation

[0068] ① Anesthesia: Calculated at 40mg / kg, intraperitoneally anesthetized with 1% sodium pentobarbital.

[0069] ②Prepare rabbit knee joint skin in a sterile environment: sterilize with 2% iodophor, make an incision about 1 cm in size along the medial edge of the patellar ligament, cut the knee joint layer by layer to expose the knee joint, and use a graduated hand to rotate on the femoral pulley Drill a 4mm diameter and 5mm depth osteochondral defect to complete the model.

[0070] 2. Animal experiment grouping:

[0071] All 18 New Zealand white rabbits were modeled and randomly divided into 3 groups after the defect was made: blank group, simple stent group and lithium-containing stent group, each with 6 rabbits.

[0072] 3. Specimen collection:

[0073] Divide the modeled rabbits in step 1 into three groups, namely a blank group, a simple stent group and a lithium-containing stent group. In the simple st...

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Abstract

The invention discloses an application of a lithium-containing natural material bioactive scaffold in osteochondral defect repair. The lithium-containing natural material bioactive scaffold adopts a bioactive glass or silk fibroin as a carrier and lithium ions as active components. The invention provides the application of the lithium-containing natural material bioactive scaffold in osteochondral defect repair. According to the application provided by the invention, the lithium-containing natural material bioactive scaffold has the functions of promoting bones and cartilages to regenerate in the osteochondral defect repair process; the traditional materials can only meet one need of bone regeneration and cartilage regeneration, while osteochondral defect needs a material to both meet the requirements for cartilage and subchondral bone regeneration; and with the adoption of the application, a manufacturing method and the sustained release characteristics of the lithium-containing scaffold are defined, the function check in vivo is also realized, and a foundation is laid for clinical applications of the lithium-containing scaffold.

Description

(1) Technical field [0001] The invention relates to the repair of osteochondral defect, in particular to the application of a lithium-containing natural material bioactive scaffold in the repair of osteochondral defect. (2) Background technology [0002] Osteochondral defect occurs in the late stage of osteoarthritis, femoral head necrosis and after osteochondritis dissecans of unknown cause. It is an orthopedic disease characterized by pathological changes of bone and cartilage at the same time. There is currently no known effective treatment. Pathological changes in cartilage or subchondral bone are caused by genetic factors, biomechanics, and immune factors alone or together, and then lead to osteochondral defects. For example, in the early stage of osteoarthritis, cartilage changes first, and then Osteochondral lesions occur with the development of the disease. In the early stage of femoral head necrosis, the subchondral bone changes first and then with the development ...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/54
Inventor 欧阳宏伟吴岩王权张小磊熊思邹晓辉
Owner ZHEJIANG UNIV
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