Double-layer temperature-sensitive gel microsphere and preparing method thereof

A technology of temperature-sensitive gel microspheres and temperature-sensitive gels, which is applied in the field of temperature-sensitive gel microspheres, gels for filling and repairing defective tissues, and can solve problems such as molecular chain damage of gels, excessive amount of organic solvents, and degradation of gel quality , to achieve the effect of slow degradation, prolonging the repair time, and simplifying the production steps

Inactive Publication Date: 2014-12-24
冯淑芹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the above shortcomings, mechanical means such as cutting, extrusion, and waste crushing have also been used to manufacture microspheres, but these conventional means will cause certain damage to the molecular chain of the gel, resulting in a decline in the quality of the gel.
Emulsion polymerization is also one of the methods for manufacturing microspheres, but the amount of organic solvent used is too much, which brings certain toxicity

Method used

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  • Double-layer temperature-sensitive gel microsphere and preparing method thereof
  • Double-layer temperature-sensitive gel microsphere and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Prepare 100ml of 2% acetic acid aqueous solution, pour it into a 250ml reactor, add 2g of chitosan and stir at room temperature until the solution becomes transparent, add 2g of NIPAAm and 2mg of KPS into the reactor. Introduce nitrogen and stir at 30°C until the reaction is complete and cool to room temperature, add 50ml of water and stir evenly, add to the attached figure 1 In the syringe (1) shown.

[0036] Prepare a mineral oil / span80 solution with a mass ratio of 80 / 20 and add it to another syringe (2). Place it on a double-rod pump and push the solutions of the two syringes through the common reaction tube (3) at a speed of 10ml / h , and then added dropwise to the 1mg / ml hyaluronic acid dilute solution containing 1% 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), with a rotating speed of 2000r / Stir at a speed of 1 min until the dropwise addition of the solution is completed, continue to stir for 30 min, and then stand still for 10 min to centrifuge and dia...

Embodiment 2

[0038] Prepare 100ml of 1% hydrochloric acid aqueous solution, pour it into a 250ml reactor, add 1g of chitosan and stir at room temperature until the solution becomes transparent, add 10g of NIPAAm and 2mg of KPS into the reactor. Introduce nitrogen and stir at 40°C until the reaction is complete and cool to room temperature, add 80ml of water and stir evenly, add to the attached figure 1 In the syringe (1) shown.

[0039] Prepare a vegetable oil / span80 solution with a mass ratio of 95 / 5 and add it to another syringe (2). Place it on a double-rod push pump, and push the solutions of the two syringes through the common reaction tube (3) at a speed of 1ml / h. Add it dropwise to the dilute solution of hyaluronic acid with 10mg / ml, stir at a speed of 3000r / min until the addition of the solution is completed, continue to stir for 30min and then stand still for 10min, then centrifuge and dialyze the solution to obtain a double layer Thermosensitive gel microspheres. The thermosen...

Embodiment 3

[0041]Prepare 100ml of 5% boric acid aqueous solution, pour it into a 250ml reaction kettle, add 0.5g chitosan and stir at room temperature until the solution becomes transparent, add 10g of NIPAAm and 2mg of azobisisobutyronitrile into the reaction kettle. Introduce nitrogen and stir at 65°C until the reaction is complete and cool to room temperature, add 50ml of water and stir evenly, add to the attached figure 1 In the syringe (1) shown.

[0042] Prepare a vegetable oil / span80 solution with a mass ratio of 90 / 10 and add it to another syringe (2). Place it on a double-rod push pump, and push the solutions of the two syringes through the common reaction tube (3) at a speed of 5ml / h. Add dropwise to the 2mg / ml hyaluronic acid dilute solution that is equipped with 10% 1-ethyl-3-(3-trimethylaminopropyl) carbodiimide (ETC), and use a rotating speed of 500r / min Stir at a high speed until the dropwise addition of the solution is completed, continue to stir for 30 minutes, then st...

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Abstract

The invention relates to a double-layer temperature-sensitive gel microsphere and a preparing method thereof. The double-layer temperature-sensitive gel microsphere and the preparing method are characterized in that: the double-layer temperature-sensitive gel microsphere adopts medicinal sodium hyaluronate and chitosan as gel raw materials, and comprises a chitosan gel core and a sodium hyaluronate gel shell. The inner layer of the gel microsphere contains the chitosan, so that the gel is compression-resistant and degradation-resistant. The outer layer of the gel microsphere is covered with the sodium hyaluronate gel shell, so that the gel microsphere is liable to inject. The particle size of the gel microsphere is small and uniform, so that the gel microsphere is liable to dialyze. The gel microsphere has synergistic effects of the sodium hyaluronate and the chitosan, so that the repairing time for an injection part is prolonged and the gel microsphere is more liable to inject. The gel microsphere is characterized by being antibacterial, anti-coagulation and good in formability in organisms.

Description

technical field [0001] The invention relates to a double-layer temperature-sensitive gel microsphere and a preparation method thereof, in particular to a temperature-sensitive gel microsphere whose surface of chitosan is coated with hyaluronic acid, which is used for filling and repairing defective tissue, and belongs to the manufacturing method of medical materials technology field. Background technique [0002] Chitosan is a non-toxic, non-irritating, very safe material for the body. Chitosan molecules contain a large number of amino (-NH2) and hydroxyl (-OH) groups, which have good cell affinity. The existence of amino groups makes chitosan have good hydrophilicity, and also makes chitosan have weak alkalinity, and its degradation can overcome the acid accumulation caused by the degradation of other polysaccharides. Chitosan has the advantages of antibacterial properties and low allergic reactions, and can be widely used in repairs of various damaged organs, healing equ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/00B01J13/14C08F251/00C08F220/54A61L27/40A61L27/20A61L27/52A61K47/36
Inventor 冯淑芹
Owner 冯淑芹
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