Scenedesmus capable of highly yielding oil as well as culture method and application thereof

A high-yield, Scenedesmus technology, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of insufficient excellent algae strains, improve the utilization rate of light energy and growth rate, and achieve good application. Prospects, effects of high biodiesel content

Inactive Publication Date: 2015-02-04
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to overcome the problem of insufficient fine algae strains used to produce microalgae oil in the prior art, and provide a high-yield oil-yielding Scenedesmus and its cult

Method used

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  • Scenedesmus capable of highly yielding oil as well as culture method and application thereof
  • Scenedesmus capable of highly yielding oil as well as culture method and application thereof
  • Scenedesmus capable of highly yielding oil as well as culture method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1 4

[0023] The isolation and identification of embodiment 1 Scenedesmus tetracerus SDEC-8:

[0024] 1. Sample collection

[0025] Water samples containing green algae were collected from a eutrophic lake in Quancheng Park, Jinan City, Shandong Province in July 2012.

[0026] 2. Isolation and purification of Scenedesmus tetracerus SDEC-8

[0027] Separation and purification step: Count the water samples in the botanical garden under the microscope by hemocytometer method, and dilute the density of the algae liquid to about 3000 / ml. On the ultra-clean test bench, pour the BG11 solid culture solution on the plate (it has been placed overnight, no bacteria growth), spray the algae liquid to be separated on the marked plate with a medical throat sprayer, then cover it, and put it on the plate. After cultivating in the incubator for more than ten days, isolated algae communities can occur on the medium, and the pure algae communities can be checked through a microscope, and then direc...

Embodiment 2 4

[0044] The growth condition optimization of embodiment 2 Scenedesmus tetracerus SDEC-8

[0045] In order to make Scenedesmus tetracerus SDEC-8 grow faster and better, the culture method, K 2 HPO 4 Dosage (22.5~84.3mg·L -1 ), culture conditions, and the combination optimization of aeration or not.

[0046] (1) The medium used is BG11

[0047] The composition of the BG11 medium is shown in Table 1. Before use, the pH was controlled at 7.1 and sterilized at 120°C for 30 minutes.

[0048] (2) Growth of Scenedesmus tetracerus SDEC-8 in BG11

[0049] After being cultured and activated for 7 days, Scenedesmus tetraceridae SDEC-8 is ready to be inoculated. The liquid of Scenedesmus tetracerides SDEC-8 to be inoculated must first be centrifuged (4000 rpm × 10 minutes, at 4°C) and washed with 15mg L -1 NaHCO 3 The solution rinses the algae liquid twice. Then, the algae should be in 1.0ml of NaHCO 3 After being resuspended in the solution, inoculate in BG11 culture medium, transf...

Embodiment 3 4

[0051] Example 3 The oil production characteristics of Scenedesmus tetracerus SDEC-8

[0052] 1. Cultivation of Scenedesmus tetracerus SDEC-8

[0053] Cultivate Scenedesmus tetracerida SDEC-8 according to the conditions and medium of Example 2, and place it in a light incubator for culture, the culture temperature is 25-30°C, full light, light intensity 2500Lux, static and aerated culture. The medium used was BG11, and the composition of the BG11 medium was shown in Table 1. Before use, the pH was controlled at 7.1, sterilized at 120° C. for 30 minutes.

[0054] 2. Determination of the growth and oil production of Scenedesmus tetraceras SDEC-8

[0055] 1. Biomass determination of Scenedesmus tetracerus SDEC-8

[0056] By observing the cell density of the algae liquid, the algae liquid is harvested when the growth stage of the algae cells enters a stable period. Determine the harvest volume, use a 50ml centrifuge tube to hold the algae liquid, put it into a large-capacity re...

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Abstract

The invention relates to scenedesmus capable of highly yielding oil as well as a culture method and application thereof. An alga variety separated from a eutrophic water region is subjected to 18S rDNA sequence analysis and microstructure morphology identification so that the alga is determined as the member of Scenedesmus sp. and the sequence number is KF999043. The alga strain is named as Scenedesmus quadricauda SDEC-8 and is preserved in China Center for Type Culture Collection on October 8, 2014; and the preservation number is CCTCC NO: M2014448. An oil production culture method comprises the following steps: separating single alga cells of the Scenedesmus quadricauda SDEC-8 and carrying out aeration enlarged cultivation on obtained pure cells under laboratory conditions. The Scenedesmus quadricauda SDEC-8 contains rich high-quality oil; and the oil content can be up to 31.8% of the dry weight of the cells and the content of saturated fatty acids in fatty acids can be up to 80%, so that the Scenedesmus quadricauda SDEC-8 can be used as the raw material of biodiesel. Based on a high cetane number (61.38) and a low iodine number (29.38gI2/100g), the biodiesel prepared by taking the microalga as the raw material has good oxidization stability.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to a high-oil-yielding Scenedesmus and its cultivation method and application. Background technique [0002] In recent years, due to the sharp increase in global consumption of fossil fuels and the reduction of petrochemical reserves, the worldwide energy crisis has been caused, coupled with the environmental problems caused by the emission of carbon dioxide from the combustion of fossil fuels, therefore, the search for a cheap green renewable energy has become It has become a scientific and social issue of widespread concern to governments and people all over the world. Studies have found that certain algae have the advantages of high oil content, easy cultivation, large yield per unit area, and no competition for land with agriculture. They are regarded as a new generation, and even the only biodiesel raw material that can replace fossil fuels. The 16-carbon and 18-carbon ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C02F3/32C12R1/89
CPCC02F3/322C02F2101/105C02F2101/16C02F2103/007C12N1/12C12P7/649C12N1/125C12R2001/89Y02E50/10
Inventor 裴海燕宋明明胡文容张硕韩琳韩飞
Owner SHANDONG UNIV
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