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Chemical preparation method of cdte quantum dot fluorescent probe for detection of trace paraquat

A fluorescent probe and quantum dot technology, applied in the field of material science, can solve the problems of limiting the number of fluorescent probes, narrow excitation spectrum, and killing

Active Publication Date: 2017-03-15
HEFEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the method of labeling target analytes with organic fluorescent dyes has also been widely used, but traditional fluorescent dyes have insurmountable defects: the excitation spectrum is narrow; the emission spectrum of fluorescent dyes is very broad, and the fluorescence peak is within 100 nm. Sometimes there is a very long tail, which causes overlap between spectral peaks, which limits the number of fluorescent probes that can be applied at the same time; organic dyes are prone to photobleaching and photolysis, and photolysis products often have a killing effect on biomolecules; biomolecules and Each organic fluorescent dye attachment requires a specific method
At present, there are no related patents and literatures on the detection of pesticide residues by quantum dot fluorescent probes based on the principle of fluorescence resonance energy transfer

Method used

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  • Chemical preparation method of cdte quantum dot fluorescent probe for detection of trace paraquat
  • Chemical preparation method of cdte quantum dot fluorescent probe for detection of trace paraquat
  • Chemical preparation method of cdte quantum dot fluorescent probe for detection of trace paraquat

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preparation example Construction

[0044] A chemical preparation method for a CdTe quantum dot fluorescent probe for detecting trace amounts of paraquat, including modifying the surface of the CdTe quantum dot fluorescent probe with a red emission band to thioglycolic acid (TGA) to make the surface With carboxyl functional groups, it is characterized in that: the surface of the CdTe quantum dots has carboxyl groups, the negatively charged carboxyl groups on the surface and the positively charged target molecule paraquat through the electrostatic interaction of positive and negative charges, when the CdTe quantum dots and the target The molecular paraquat is close to each other in space. Through the principle of fluorescence resonance energy transfer, the emission spectrum of the CdTe quantum dot fluorescent probe is a red emission band that can be absorbed by the green target molecule paraquat. Using the change of the fluorescence intensity of the CdTe quantum dots, the realization For the detection of trace par...

Embodiment

[0048] Example: According to the interaction of positive and negative charges, using the prepared CdTe quantum dots as a support, a two-step reaction can be used to obtain a CdTe quantum dot fluorescent probe rich in carboxyl groups on the surface.

[0049] 1.1 The first step is the preparation of purple transparent NaHTe solution: First, weigh 0.12 g ~ 0.13 g tellurium powder and 0.07 g ~ 0.09 g sodium borohydride respectively, put them in a 50 mL three-necked flask, and then use a 1 mL micro injection Add 2 mL~4 mL deionized water to a 50 mL three-necked flask, and react at 25°C in a helium atmosphere until the black tellurium powder disappears, and a purple transparent NaHTe solution is obtained;

[0050] 1.2 The second step is to modify the surface of the red CdTe quantum dot fluorescent probe with thioglycolic acid in the emission spectrum: weigh 0.4 g ~ 0.5 g CdCl 2 2.5H 2 Dissolve O in a 50 mL three-neck flask, add 20 mL ~ 25 mL deionized water, sonicate for 3 ~ 5 minu...

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Abstract

A chemical preparation method for a CdTe quantum dot fluorescent probe for detecting a trace amount of paraquat comprises the step of modifying thioglycollic acid on the surface of the CdTe quantum dot fluorescent probe emitting a red emission spectrum band so as to enable the CdTe quantum dot fluorescent probe is provided with carboxyl functional groups on the surface. The preparation process comprises the following two specific steps: firstly, preparing a NaHTe solution; then, synthesizing CdTe quantum dots of which the surfaces are modified with thioglycollic acid, and adjusting the pH value to be 10-12 with KOH, so as to obtain the CdTe quantum dot fluorescent probe. According to the invention, carboxyl with negative charge on the surfaces of the CdTe quantum dots and target paraquat molecules with positive charge have the electrostatic interaction through the positive charge and negative charge, when the CdTe quantum dots and the paraquat approach to each other in space, the red spectrum band emitted by the CdTe quantum dot fluorescent probe can be absorbed by green target analyte paraquat modules through the fluorescence resonance energy transfer principle, and the detection of a trace amount of paraquat can be implemented through the utilization of the variation of the CdTe quantum dot fluorescence intensity.

Description

technical field [0001] The invention relates to the field of material science, in particular to a chemical preparation method of a CdTe quantum dot fluorescent probe capable of detecting trace amounts of paraquat. Background technique [0002] Pesticides play a huge role in the prevention and control of agricultural diseases, insects and weeds, and in ensuring high yields of crops. However, the uncontrolled use of pesticides poses a serious threat to my country's ecological environment and human health. In particular, pesticide residues in agricultural and animal husbandry products and food have chronic toxicity, "three causes" (carcinogenic, teratogenic, mutagenic) effects and environmental hormone effects on the human body. Incidents of poisoning directly caused by residues of highly toxic pesticides in agricultural products also occur from time to time. Pesticide residues pose a long-term serious threat to my country's food safety. In recent years, the public's sense of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 高大明孙虹陈红张凌云管航敏王先琴耿娟娟刘陈未朱德春
Owner HEFEI UNIV
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