Method for measuring photosynthetic rate of CAM plants

A photosynthetic rate and plant technology, which is applied in the field of measuring the photosynthetic rate of CAM plants, and can solve problems such as the inability to accurately measure the photosynthetic rate of CAM plants

Inactive Publication Date: 2015-02-18
CHINA AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for accurately measuring the photosynthetic rate of CAM plants

Method used

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  • Method for measuring photosynthetic rate of CAM plants
  • Method for measuring photosynthetic rate of CAM plants
  • Method for measuring photosynthetic rate of CAM plants

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1 The method for measuring the photosynthetic rate of CAM plants

[0023] Plant species: big leaves take root ( daigremontiana), Kalanchoe ( blossfeldiana), Sansevieria trifasciata, and Schlumbergera altensteinii, the above four test plants are all shown as typical obligate CAM plants in the prior art.

[0024] Measurement environment: artificial light environment (artificial climate chamber).

[0025] Measurement period: 2 days.

[0026] The specific measurement method includes the following steps:

[0027] 1) After connecting the main body of the photosynthetic measuring device and the pipeline and connection line between the leaf chamber, carry out CO2 in the empty leaf chamber without placing any measurement samples. 2Zeroing correction of absorption;

[0028] 2) The temperature control mode of the leaf chamber is set to constant temperature mode;

[0029] 3) Adjust the air flow rate of the gas path to achieve the air temperature control target, a...

Embodiment 2

[0035] Embodiment 2 The method for measuring the photosynthetic rate of facultative CAM plants

[0036] Plant species: Dendrobium officinale Kimura et Migo, a rare and endangered medicinal plant, is also a CAM plant, but the existing literature fails to identify whether it is an obligate CAM plant or a facultative CAM plant. The plant has extremely high medicinal value and economic value. After more than 10 years of research, the inventors have shown that Dendrobium officinale is a typical facultative CAM plant, and under normal living conditions, the CAM photosynthetic pathway and the C3 photosynthetic pathway exist simultaneously to perform photosynthesis.

[0037] Measurement environment: artificial light environment (artificial climate chamber) and natural light environment (greenhouse).

[0038] Measurement period: 4 days.

[0039] The specific measurement method includes the following steps:

[0040] 1) After connecting the main body of the photosynthetic measuring de...

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Abstract

The invention provides a method for measuring the photosynthetic rate of CAM plants. The method is based on continuous monitoring on CO2 in a large leaf chamber, the dynamic change in net CO2 exchange capacity of CAM plants is measured, and then photosynthetic characteristic indicators of CAM plants, such as the net photosynthetic rate and the CO2 absorption rate in the dark period, can be measured accordingly. The method is characterized in that the net CO2 exchange capacity is continuously measured based on the stomatal movement characteristics of CAM plants, so that the actual assimilation efficiency or the photosynthetic characteristics of CAM plants can be measured in a diurnal cycle environment or an environment where light and shade alternately change. The method for measuring photosynthetic rate is applied to not only CAM plants but also tissue-cultured plants and plants with weak photosynthesis, effectively solves the problem of accurate measurement of the photosynthetic characteristics of CAM plants, and provides a solution to measure the photosynthetic characteristics of tissue-cultured plants and plants with weak photosynthesis. The method facilitates productization and industrial production, and has good application and business prospects.

Description

technical field [0001] The invention relates to the field of photosynthetic measurement, in particular to a method for measuring the photosynthetic rate of CAM plants. Background technique [0002] Some typical CAM plants, such as orchids, desert plants, and succulents, have formed nighttime stomatal openings to absorb CO in order to adapt to special living environments such as large temperature differences between day and night, drought, and strong light. 2 , closed during the day to prevent excessive transpiration, aerial roots can directly absorb water in the air and other special physiological mechanisms, and its CAM photosynthetic pathway is significantly different from the common C3 photosynthetic pathway or C4 photosynthetic pathway. Photosynthetic measurement is an important method to study the physiological ecology and growth and development characteristics of plants, and is a common means of scientific research in the fields of botany, plant environmental physiolog...

Claims

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Application Information

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IPC IPC(8): G01N21/84
Inventor 贺冬仙郑国明高荣孚杜维芬
Owner CHINA AGRI UNIV
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