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Kit for simultaneously and rapidly measuring content of glucose, fructose and total sugar and application of kit

A technology for rapid determination of total sugar content, applied in color/spectral characteristic measurement, analysis using chemical indicators, etc., can solve the problem of difficulty in adapting to rapid and large-scale detection requirements, inability to detect multiple sugars at the same time, complex and cumbersome operations, etc. problems, achieve the effect of low cost, simple method and large amount of sample processing

Inactive Publication Date: 2015-02-18
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods widely used at present have problems such as high detection cost, complicated and cumbersome operation, long experimental cycle or inability to detect multiple sugars at the same time, and it is difficult to meet the needs of rapid and large-scale detection.

Method used

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  • Kit for simultaneously and rapidly measuring content of glucose, fructose and total sugar and application of kit
  • Kit for simultaneously and rapidly measuring content of glucose, fructose and total sugar and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation of sugar standard (reagent I), o-toluidine reagent (reagent II), resorcinol reagent (reagent III) and phenol reagent (reagent IV):

[0020] Reagent I: Dissolve 0.0100 g of glucose, fructose and sucrose standard substances in distilled water immediately before use and make up to 100 mL to prepare a 0.1 mg / mL standard solution; Reagent II: Dissolve 0.1 g of thiourea in 75 mL of glacial acetic acid Add o-toluidine 1mL, saturated boric acid 4mL, mix well, add glacial acetic acid to 100mL, store in a brown bottle; Reagent Ⅲ: dissolve 0.1 g resorcinol in 100mL hydrochloric acid solution, the hydrochloric acid solution used Water and concentrated hydrochloric acid are prepared at a volume ratio of 2:1; Reagent IV: Dilute 0.1g of phenol with distilled water to 50mL.

Embodiment 2

[0021] Example 2 Preparation of sugar standard (reagent I), o-toluidine reagent (reagent II), resorcinol reagent (reagent III) and phenol reagent (reagent IV):

[0022] Reagent I: Dissolve 0.5 g of glucose, fructose and sucrose standard substances in distilled water before use and make the volume to 100 mL to prepare a 5 mg / mL standard solution; Reagent II: Dissolve 0.5 g of thiourea in 75 mL of glacial acetic acid , then add o-toluidine 15mL, saturated boric acid 4mL, mix well, add glacial acetic acid to 100mL, store in a brown bottle; reagent Ⅲ: dissolve 1.000 g resorcinol in 100mL hydrochloric acid solution, the hydrochloric acid solution used is water Prepared with concentrated hydrochloric acid at a volume ratio of 2:1; Reagent IV: Dilute 5.000 g of phenol with distilled water to 50 mL.

Embodiment 3

[0023] Example 3 Preparation of sugar standard (reagent I), o-toluidine reagent (reagent II), resorcinol reagent (reagent III) and phenol reagent (reagent IV):

[0024] Reagent I: Dissolve 10 g of glucose, fructose and sucrose standard substances in distilled water before use and make the volume to 100 mL to prepare a 100 mg / mL standard solution; Reagent II: Dissolve 1 g of thiourea in 75 mL of glacial acetic acid , then add o-toluidine 30mL, saturated boric acid 4mL, mix well, add glacial acetic acid to 100mL, store in a brown bottle; reagent Ⅲ: dissolve 2g resorcinol in 100mL hydrochloric acid solution, the hydrochloric acid solution used is water and Concentrated hydrochloric acid is prepared at a volume ratio of 2:1; Reagent IV: Dilute 10 g of phenol with distilled water to 50 mL.

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Abstract

The invention discloses a kit for simultaneously and rapidly measuring content of glucose, fructose and total sugar and an application method of the kit. The kit is developed based on a principle that carbohydrates are dehydrated to generate hydroxymethyl furfural under the acid action and the hydroxymethyl furfural and a developing agent are condensed to form a colored compound. The kit comprises four reagents, wherein the reagent I refers to a mixed standard substance of glucose, fructose and cane sugar; the reagent II refers to an ortho-toluidine reagent and is used for measuring the content of glucose; the reagent III refers to a resorcinol reagent and is used for measuring the content of fructose; and the reagent IV refers to a phenol reagent and is used for measuring the content of total sugar. The kit is used for simultaneously measuring the content of glucose, fructose and total sugar, has the characteristics of high specificity and high sensitivity, can realize high-flux and rapid detection of the glucose, fructose and total sugar, is large in sample treatment amount and relatively low in cost, is suitable for measuring the content of glucose, fructose and total sugar in honey, fruits and vegetables and other samples and is particularly suitable for large-scale field sample analysis.

Description

technical field [0001] The invention belongs to the field of detection and analysis of food active ingredients, and in particular relates to a kit for simultaneously and rapidly measuring the contents of glucose, fructose and total sugar and an application method thereof. Background technique [0002] The sugar content in honey is one of the main bases for evaluating the quality of honey. "National Food Safety Standard Honey" (GB14963-2011) stipulates the content requirements of sugar in honey, which uses liquid chromatography differential refraction detection method to determine the content of sugars such as fructose and glucose in honey (GB / T 18932.22). In addition, the detection methods of sugar in honey also include titration, optical rotation, ultraviolet-visible spectrophotometry, near-infrared spectroscopy, etc. However, these methods widely used at present have problems such as high detection cost, complex and cumbersome operation, long experimental cycle, or inabil...

Claims

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Application Information

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IPC IPC(8): G01N31/22G01N21/31
Inventor 史培颖姚宏缪晓青
Owner FUJIAN AGRI & FORESTRY UNIV
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