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Application of ruxolitinib in the preparation of drugs for the treatment of M2 type acute myeloid leukemia
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A technology for acute myeloid leukemia, applied in the field of application of Ruxolitinib in the preparation of drugs for the treatment of M2 type acute myeloid leukemia
Active Publication Date: 2017-01-18
THE FIFTH PEOPLES HOSPITAL OF SHANGHAI FUDAN UNIV
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Problems solved by technology
However, there is no report about Ruxolitinib as a drug for the treatment of leukemia, especially for the treatment of M2 acute myeloid leukemia.
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Embodiment 1
[0048] Example 1 In vitro effects of Ruxolitinib and / or rsTRAIL on Kasumi-1 cells
[0052] Kasumi-1 cells: donated by Professor Zhu Jiang from the Institute of Blood Research of Ruijin Hospital, Shanghai, using a medium containing 10% FBS + RPMI1640 at 37°C, 5% CO 2 , Cultivate in an incubator under saturated humidity, and take log-phase cells for experiment.
[0061] Cell culture conditions: Kasumi-1 cells were inoculated in RPMI1640 medium containing 10% FBS under aseptic conditions, at 37℃, 5% CO 2 Culture in an incubator.
[0062] Cell passage: After the cells are centrifuged at low speed, the supernatant is discarded, the cells are resuspended in 3-5ml medium, and transferred to the culture flask to continue the culture.
[0114] Example 2 Ruxolitinib enhances the mechanism of rsTRAIL on Kasumi-1 cell apoptosis in vitro
[0115] 1. Materials and methods
[0116] 1.1 Material
[0117] 1.1.1 Primer
[0118] According to the human TRAIL, DR4, DR5, DcR1, DcR2, JAK1, JAK2, and mRNA sequences provided by the NCBI database, with β-actin as an internal reference, primers were designed with the software Primer5.0, and synthesized by Shanghai Shenggong Biological Co., Ltd.:
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Abstract
The invention relates to use of Ruxolitinib in preparation of a drug for treating M2 type acute myeloid leukemia with t (8; 21) chromosome translocation, and the in-vitro effective concentration is 1 * 10<-6>-1 *10<-5>M. DrugRuxolitinib is not only itself has the effect of inhibiting growth and inducing apoptosis on leukemiacell Kasumi-1, and enhances the drug sensitivity of the leukemiacell Kasumi-1 on rsTRAI by up-regulation of mRNA and protein expression level of DR4, up-regulation of cellapoptosisprotein Bax expression, activation of apoptosisproteincaspase-3 and inhibition of NF-kappa B protein activity. The drug can be used for treating the M2 type acute myeloid leukemia with t (8; 21) chromosome translocation, the new drug for treating the M2 type acute myeloid leukemia with t (8; 21) chromosome translocation is provided, and a new approach for overcoming TRAIL resistance in leukemia cells can be provided.
Description
Technical field [0001] The invention relates to the application of Ruxolitinib in the preparation of a medicine for the treatment of M2 acute myeloid leukemia, in particular to the application of Ruxolitinib in the preparation of a medicine for the treatment of M2 acute myeloid leukemia with t(8; 21) chromosome translocation. Background technique [0002] Leukemia is a malignant clonal disease of hematopoietic stem and progenitor cells. Leukemia cells stop at different stages of cell development due to increased self-renewal, uncontrolled proliferation, differentiation disorders and apoptosis of leukemia cells. In bone marrow and other hematopoietic tissues, leukemia cells proliferate and accumulate in large quantities, which inhibits normal hematopoiesis and infiltrates other organs and tissues. The clinical manifestations are hepatosplenic lymphadenopathy or local masses accompanied by varying degrees of anemia, bleeding, infection and bone pain . Leukemia is a common disease,...
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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/519A61K38/17A61P35/02
CPCA61K31/519A61K38/191A61K2300/00
Inventor 刘立根庄晏
Owner THE FIFTH PEOPLES HOSPITAL OF SHANGHAI FUDAN UNIV
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