Cordyceps sinensis liquor, preparation and application thereof
A technology for cordyceps wine and cordyceps, which is applied to cordyceps wine and its preparation and application fields, can solve the problems of single finished product form, culture application, long production cycle, etc. Effect
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Embodiment 1
[0066] 1. Experimental strains
[0067] The selected bacterial species in this experiment is the anamorph of Cordyceps militaris: Isaria militaris, also known as Paecilomyces militaris (widely distributed species, can be purchased in many edible fungus strain factories, or After collecting Cordyceps militaris, it is isolated according to the microbial isolation method).
[0068] 2. Cultivation of Cordyceps militaris
[0069] 1) Level 1 slanted seeds
[0070] Choose tussah silkworm chrysalis with bright color and strong growth. It is required to be free from germ infection, mildew, and insects. Put tussah silkworm chrysalis into water and soak for 8 hours, then put them into test tubes, sterilize them at 105-120°C for 30 minutes, take them out, and inoculate them after cooling. Connect the Paecilomyces militaris strain to the pupal body, inoculate as evenly as possible, and put it into a 25°C incubator for about 7 days. It can be used for secondary seed liquid culture after...
Embodiment 2
[0087] 1. Experimental strains
[0088] The strain used in this experiment is Cordyceps takaomontana. (Widespread species, which can be purchased at the Microbial Collection Center of the Chinese Academy of Sciences, or obtained after collecting Cordyceps Kaohsiung and isolated according to the microbial separation method)
[0089] 2. Cultivation of Cordyceps
[0090] 1) Level 1 slanted seeds
[0091] Choose tussah silkworm chrysalis with bright color and strong growth. It is required to be free from germ infection, mildew, and insects. Put tussah silkworm chrysalis into water and soak for 8 hours, then put them into test tubes, sterilize them at 105-120°C for 30 minutes, take them out, and inoculate them after cooling. Inoculate the bacteria of Cordyceps takaomontana on the pupae, inoculate as evenly as possible, and culture them in a 25°C incubator for about 7 days. It can be used for secondary seed liquid culture after the insect body is covered with hyphae.
[0092] 2...
Embodiment 3
[0108] 1. Experimental strains
[0109] The strain used in this experiment is Isaria cicadae. (Widespread species, which can be purchased at the Microbial Collection Center of the Chinese Academy of Sciences, or obtained by collecting cicadas by oneself and separated by microbial separation methods)
[0110] 2. Cicada Cultivation
[0111] 1) Level 1 slanted seeds
[0112] Choose tussah silkworm chrysalis with bright color and strong growth. It is required to be free from germ infection, mildew, and insects. Put tussah silkworm chrysalis into water and soak for 8 hours, then put them into test tubes, sterilize them at 105-120°C for 30 minutes, take them out, and inoculate them after cooling. Connect the cicadae strains to the pupal body, inoculate as evenly as possible, and put them into a 25°C incubator to cultivate for about 7 days. It can be used for secondary seed liquid culture after the insect body is covered with hyphae.
[0113] 2) Secondary seed cultivation
[01...
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