Nanofiber foam-based protein adsorptive separation device and preparation method thereof

Abstract

The invention provides a nanofiber foam-based protein adsorptive separation device and a preparation method thereof. The nanofiber foam-based protein adsorptive separation device is characterized by comprising an open pore protection cover, wherein the open pore protection cover is internally provided with a nanofiber foam-based protein adsorption material; a liquid collection capillary network is arranged in the nanofiber foam-based protein adsorption material; the liquid collection capillary network is connected with a main infusion tube; and the main infusion tube is connected with a liquid collection tank through a pump. The nanofiber foam-based protein adsorptive separation device has the advantages of simple preparation process, low production cost, convenience in operation and strong expandability; and the protein adsorption material has the advantages of high mass transfer rate, high adsorption rate, high adsorption capacity, high protein dissolution rate and good pressure resistant property and the proteins in the solution can be intensively adsorbed onto the material through a direct extraction manner to realize the batch efficient continuous separation and adsorption of the protein-containing solution; therefore, the nanofiber foam-based protein adsorptive separation device has wide application prospect in the fields of biological pharmacy and the like.

Description

technical field

[0001] The invention belongs to the technical field of protein adsorption and separation, and in particular relates to a nanofiber foam-based protein adsorption and separation device capable of realizing rapid adsorption and separation of proteins and a preparation method thereof. Background technique

[0002] Efficient separation and purification of proteins is one of the key technologies in modern drug analysis, analytical chemistry and life science research. The existing protein separation and purification methods mainly include: dialysis, ultrafiltration, density gradient centrifugation, and gel filtration based on protein molecular size differences; isoelectric point precipitation, salt dissolution and salting out methods based on protein solubility differences , low temperature organic solvent precipitation method; electrophoresis method based on the difference in the charge of the protein, ion exchange chromatography separation method; affinity chromat...

Images